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The map label for this gene is nagE [H]

Identifier: 147675085

GI number: 147675085

Start: 552060

End: 553631

Strand: Direct

Name: nagE [H]

Synonym: VC0395_A0516

Alternate gene names: 147675085

Gene position: 552060-553631 (Clockwise)

Preceding gene: 147675715

Following gene: 147674963

Centisome position: 18.26

GC content: 49.05

Gene sequence:

>1572_bases
GTGAATATTCTTGGATATGCGCAAAAGTTAGGTAAAGCATTGATGCTACCTATCGCTACGCTACCTGTAGCGGCGCTCTT
ACTTCGTTTAGGTCAACCAGACCTGTTAGGCATTCCATTCATGGCTGCAGCGGGCGATGCAATTTTTAGCCAGCTGCCAC
TACTGTTTGGTTTGGGTATTGCCATTGGTTTATCGAAAGATGGATCTGGCGCCGCAGGTCTAGCGGGTGCAGTAGCTTAC
TTTGTTCTGAGTGCAACGGCAAAAACGATTGATGCTTCAATCAACATGTCTTTCTTTGGCGGTATCTTCGCAGGTATCAT
CGCAGGGCACTCTTACAACGCGTTCCACACTACACGTCTGCCAGAGTGGCTAGCATTCTTTGCTGGTAAGCGTTTAGTGC
CAATTATGGCGGGTCTGTTCGCTCTGGTTGCTGGTGCGCTTTCTGGCGTTATCTGGCCATCAATTCAAGGTGGATTAGAT
GCGCTGGCGCATGCCGTCTCTACATCAGGTGCTTTAGGCCAATTTGTTTACGGTACTCTGAACCGTGCTCTGATCCCTGT
AGGTTTACACCACGTTCTGAACTCTTACTTCTGGTTCGGCATGGGTACCTGTCAAGAGATCCTAGTTTCAGGTGCTCAAG
CCGCTGGTCAAGCACTGCCTGCACTCGAAAAACTGTGTGTGGATCCAGCTCTAGCGAAAACCCTAGTTGTGGGTCAAGAG
CATACTTTTGCGTTCAAAAATGCGGTAACGCCTGAAATCACAGCTGTGGTAAAAGAAGTGACTGAAGTGGTTAAATCTGG
CGACCTACACCGTTTCTTCGGCGGCGATAAGAGCGCTGGGGTATTCATGAACGGCTTCTTCCCTGTCATGATGTTCGGTC
TACCAGGTGCTGCACTTGCTATGTACCTAGCCGCTCCAGCTGAGCGTCGTCACCAAGTGGGTGGTGCACTGTTCTCTGTT
GCGTTCTGTTCATTCCTAACAGGTATCACTGAGCCTCTAGAGTTCATGTTTGTGTTCCTAGCACCTGCGCTGTATGCAAT
GCATGCGGTATTTACAGGTCTGTCACTGGTTGTGGCTAACATGTTTGGCACACTGCATGGTTTCGGTTTCTCAGCAGGTC
TTATCGACTTCCTGTTGAACTGGGGTCTAGCAACGAAACCTGCAACTCTGTTTGTGATTGGTCTGGCGTTCTTTGCTCTG
TACTTCTTTACGTTCACATTCGCCATTCGTGCGTTCAACCTGAAATCTCCAGGTCGTGAAGACGAAGAAGTGGAAACAGA
AACAGACTCCGCTGATGCATCTAAAGGTGATTTGGCTCGCCAATACCTGAAAGCACTGGGTGGTCATGAAAACCTGACAT
CTATCGATGCGTGCATCACACGTCTACGTCTGACTCTGAAAGACCGCTCTGTTGCAGATGAAGCGGTACTGAAGAAACTG
GGCGCGAAAGGTGTTGTGAAACTGGGTGAAAATAACCTGCAAGTTATCCTTGGCCCTCTAGCAGAAATCGTCGCGGGTGA
GATGAAAGCCATCAGCCCGAAAGAAGATCTTTCTGACGTTAAACTGCCTTAA

Upstream 100 bases:

>100_bases
TTGCGGAGCAAAATTAATGTCATAAACTGACCAGGACTAAATTGGGCGACTAAAAAAAGTCTCTCAATACTATACAAAAA
TCCTATAGGGGGAACTTAAG

Downstream 100 bases:

>100_bases
TCGAGTTTAAACCCTAGCCTGAATCAAAGCCTTCGCGAAAGCGAAGGCTTTTTACTTATTTCCCGTTTGAGAAGTGGCGA
TATAAGCATCGAACCGTAGA

Product: PTS system, N-acetylglucosamine-specific IIABC component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MNILGYAQKLGKALMLPIATLPVAALLLRLGQPDLLGIPFMAAAGDAIFSQLPLLFGLGIAIGLSKDGSGAAGLAGAVAY
FVLSATAKTIDASINMSFFGGIFAGIIAGHSYNAFHTTRLPEWLAFFAGKRLVPIMAGLFALVAGALSGVIWPSIQGGLD
ALAHAVSTSGALGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEILVSGAQAAGQALPALEKLCVDPALAKTLVVGQE
HTFAFKNAVTPEITAVVKEVTEVVKSGDLHRFFGGDKSAGVFMNGFFPVMMFGLPGAALAMYLAAPAERRHQVGGALFSV
AFCSFLTGITEPLEFMFVFLAPALYAMHAVFTGLSLVVANMFGTLHGFGFSAGLIDFLLNWGLATKPATLFVIGLAFFAL
YFFTFTFAIRAFNLKSPGREDEEVETETDSADASKGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
GAKGVVKLGENNLQVILGPLAEIVAGEMKAISPKEDLSDVKLP

Sequences:

>Translated_523_residues
MNILGYAQKLGKALMLPIATLPVAALLLRLGQPDLLGIPFMAAAGDAIFSQLPLLFGLGIAIGLSKDGSGAAGLAGAVAY
FVLSATAKTIDASINMSFFGGIFAGIIAGHSYNAFHTTRLPEWLAFFAGKRLVPIMAGLFALVAGALSGVIWPSIQGGLD
ALAHAVSTSGALGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEILVSGAQAAGQALPALEKLCVDPALAKTLVVGQE
HTFAFKNAVTPEITAVVKEVTEVVKSGDLHRFFGGDKSAGVFMNGFFPVMMFGLPGAALAMYLAAPAERRHQVGGALFSV
AFCSFLTGITEPLEFMFVFLAPALYAMHAVFTGLSLVVANMFGTLHGFGFSAGLIDFLLNWGLATKPATLFVIGLAFFAL
YFFTFTFAIRAFNLKSPGREDEEVETETDSADASKGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
GAKGVVKLGENNLQVILGPLAEIVAGEMKAISPKEDLSDVKLP
>Mature_523_residues
MNILGYAQKLGKALMLPIATLPVAALLLRLGQPDLLGIPFMAAAGDAIFSQLPLLFGLGIAIGLSKDGSGAAGLAGAVAY
FVLSATAKTIDASINMSFFGGIFAGIIAGHSYNAFHTTRLPEWLAFFAGKRLVPIMAGLFALVAGALSGVIWPSIQGGLD
ALAHAVSTSGALGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEILVSGAQAAGQALPALEKLCVDPALAKTLVVGQE
HTFAFKNAVTPEITAVVKEVTEVVKSGDLHRFFGGDKSAGVFMNGFFPVMMFGLPGAALAMYLAAPAERRHQVGGALFSV
AFCSFLTGITEPLEFMFVFLAPALYAMHAVFTGLSLVVANMFGTLHGFGFSAGLIDFLLNWGLATKPATLFVIGLAFFAL
YFFTFTFAIRAFNLKSPGREDEEVETETDSADASKGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
GAKGVVKLGENNLQVILGPLAEIVAGEMKAISPKEDLSDVKLP

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=521, Percent_Identity=47.6007677543186, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1787343, Length=527, Percent_Identity=35.2941176470588, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1787908, Length=555, Percent_Identity=33.8738738738739, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 55105; Mature: 55105

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILGYAQKLGKALMLPIATLPVAALLLRLGQPDLLGIPFMAAAGDAIFSQLPLLFGLGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AIGLSKDGSGAAGLAGAVAYFVLSATAKTIDASINMSFFGGIFAGIIAGHSYNAFHTTRL
HEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHCH
PEWLAFFAGKRLVPIMAGLFALVAGALSGVIWPSIQGGLDALAHAVSTSGALGQFVYGTL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
NRALIPVGLHHVLNSYFWFGMGTCQEILVSGAQAAGQALPALEKLCVDPALAKTLVVGQE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
HTFAFKNAVTPEITAVVKEVTEVVKSGDLHRFFGGDKSAGVFMNGFFPVMMFGLPGAALA
CHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEHHHHHHHHHCCCHHHHH
MYLAAPAERRHQVGGALFSVAFCSFLTGITEPLEFMFVFLAPALYAMHAVFTGLSLVVAN
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFGTLHGFGFSAGLIDFLLNWGLATKPATLFVIGLAFFALYFFTFTFAIRAFNLKSPGRE
HHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DEEVETETDSADASKGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
CCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAKGVVKLGENNLQVILGPLAEIVAGEMKAISPKEDLSDVKLP
CCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCHHCCCCCCCC
>Mature Secondary Structure
MNILGYAQKLGKALMLPIATLPVAALLLRLGQPDLLGIPFMAAAGDAIFSQLPLLFGLGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AIGLSKDGSGAAGLAGAVAYFVLSATAKTIDASINMSFFGGIFAGIIAGHSYNAFHTTRL
HEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHCH
PEWLAFFAGKRLVPIMAGLFALVAGALSGVIWPSIQGGLDALAHAVSTSGALGQFVYGTL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
NRALIPVGLHHVLNSYFWFGMGTCQEILVSGAQAAGQALPALEKLCVDPALAKTLVVGQE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
HTFAFKNAVTPEITAVVKEVTEVVKSGDLHRFFGGDKSAGVFMNGFFPVMMFGLPGAALA
CHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEHHHHHHHHHCCCHHHHH
MYLAAPAERRHQVGGALFSVAFCSFLTGITEPLEFMFVFLAPALYAMHAVFTGLSLVVAN
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFGTLHGFGFSAGLIDFLLNWGLATKPATLFVIGLAFFALYFFTFTFAIRAFNLKSPGRE
HHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DEEVETETDSADASKGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
CCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAKGVVKLGENNLQVILGPLAEIVAGEMKAISPKEDLSDVKLP
CCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]