Click here to switch to the map view.

The map label for this gene is mbaA [H]

Identifier: 147674861

GI number: 147674861

Start: 242024

End: 244399

Strand: Reverse

Name: mbaA [H]

Synonym: VC0395_A0233

Alternate gene names: 147674861

Gene position: 244399-242024 (Counterclockwise)

Preceding gene: 147674245

Following gene: 147673906

Centisome position: 8.08

GC content: 44.99

Gene sequence:

>2376_bases
ATGAAGCTAAACCATAGAATTCTGTTACTGATTGCACCGATGATCCTGCTCAGTGCAGCCGCATCAAGCTACATCATTTA
CACCAGTCAGAAAAATGCGCTGCTAAAAAGAACCGACAGTTACCTGCAGTTGAATATTGAAAAGTTAGCCAGCCACTATC
GACAAGCTCAGTCGCTCGTCAGCAGTTATGCCTTCACGCTGGCCAAAAGTGACATCATCCGCCACTACTTCTCTTTAGAA
AAAAATCCTTACCGCGAACTAGAACTGGTGGATAACTTACGTGAAACCCTGCAAATTTTGCAGCCGAATGAAAAACAGCT
CGTGTCGTTATCGATCCTCAATGGCCATGAAGAGCTGCTTTATTATGCGGAAAATAGCTCCGATCCATTTGCCGAGCTTG
ACCCTAAGGTGATGGCGTACATCAAACAGCGTTTTGCATCCACACAAAAAAACTCTGATATCAGCTACACCGTCAACTCA
GCGGGTGAAGATATTTTGGTTCGCTACGATATGTTGGATACTCAGACACTCTCAACGCCGCTCAGTTATAACCGCCAAGA
CGTGTTTTTTGTGGTGGTGTATGTGGTGCTTGAGCAGTTCAGCCAACTGCGTAAAAAAATTGAATTTGATAACCAGAGCC
CGATATTTTTTACGCATTCACCTCCGAGCTACCGCACGGGCTTATTGCAATCGGTTGAATTACAACCGGGCTTTTACGCC
ATACTCGACCCTGCACCGAAACTGATTAACGCCCAGCTTCACTCCATTCAGCGCGAACTTTTACTCTCTTTTGGCGTTTC
GGCACTGGTGACGGTACTCATGCTACTGCTCCTTCTCTATCGCCATGTGATCAATCCGATCCTGCATTTGGATAAACAGC
TTGAAGAGGTGGAAAACAACCAACGTAAGAACATTGAAAAACTCAATACTGACGATGAAATTGGGCGGCTTTCCTCGCGT
TTTTACGCTATGTACAGCGAACTTCATTCAACCTATCAACGCACGAAAGCACTTGCGGAAAACGATCACCTCACCAAGCT
GGCTAACCGCTATCAATTTCAAGTTCAAGCCGATCTGCTGCTATCACGTTGTTACGATACTCAGCATATCTGGGTCATGT
ATATCGACTTAGATAACTTCAAGTACGTTAATGATAAGTATGGTCACCAAATCGGTGATTCCTTACTGGTCAGCTTCGCG
ACCCACGTTCGCCAGTTATGTAAAAATTTTGAGGCCAGCCATAACACCTACAGTATTGCGGCCCGTTTATCTGGTGATGA
GTTTGCTATTTTGCTCGTCTCCCCCAAACGGTTTAATGATTGTGCGAAGATCTTTGCTCAACGCTTGTTGGCACCGATCC
AAAATAAAGACAACTCACCACTTAGCCATTTTCCCATTACTGCCAGTATTGGTATTGCGACTTTCCCTAAAGATGGCGAA
CACATTGAGAAATTGCTGCTCAATGCCGATACCGCCATGTATCAAGCGAAAAATGCGGGCAAAAACCAAGTCGCTTACTA
TTCGCAGGCGCTAGATCAAATAGTACAGCGGCGCAATAATATTGAACGAGCACTGCGCTTAGGGTTATTTGATCAAGAGT
TCAATTTGGCTTATCAACCTTACTTTACTTGCTCAGGGAAACGCTTAGTGGGCTTCGAAGTATTGCTGCGCTGGCAATCT
GAATTGCTCGGTGAAGTGTCGCCAGAAGAGTTCATTCCAATTGCAGAGCAAACTGGATTGTTTGGCACGATCGACCGCTG
GGTCATTTCAAAAGCTTTTCAAGAGATATCAACTCTACAAGCCATAGTGAAAGAGCCGATCCAGGTGTCGATCAACCTCT
CTTCGGCAGAACTCAATTCGCTGAAATTGGCTCAATTTATCCATCGACAGGCTGAGCAGTTTGGCGTTTCTCCCGCATGG
ATCGATTTTGAAATCACAGAAACGTTTGCCGCAGATTCGCAAAGCTTCCCATTGCTGCACGAACTCTCGCGTCTTGGCTA
TGGGTTAACGATTGATGATTTTGGCTCAGGCTATACCTCTATTACTCAGTTGGTGCAGTATCCAGTACAAAAGATTAAGT
TTGATCGCCATTTTTTGGATACACTCATCGCCACCAACAAACAAAATGTTATTCGTCCACTGATTGATCTCTGCCACTCT
CAATCCATGAAAGTGACCGCTGAAGGCATTGAGAGTGAAACTATGCATCAATGGTTGGCCGATTATGAGTGCGATTACAT
GCAAGGCTTCTACTTTGGTTATCCAATGAGCCTAAGCGAGATCTCCCCATGGCTTCATGCCTCCAATCATAAAAAGAAGA
GTTATGCGCAAGATCATTATTGCTTCACAGAACCCAGCCAAAGTGAATGCCGTTAG

Upstream 100 bases:

>100_bases
CTCCCAGAGCAGCGATTGGCGGAGGGAATAATCTACAGCGAACTCTCGGCGAAAAACCTCAGTCTGCGTGCCAAAATCAT
CAGCAGTGTGACTTACCAAT

Downstream 100 bases:

>100_bases
AAGTGCCTTTAGCACCGTATTTCCAGATCAAGAATGGGAATTTATTGGCGTATCAGTACCGAGCGAAGTCGCCGATCAGC
CCATGAGTGACGAGGAAACC

Product: signal protein domain/GGDEF domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 791; Mature: 791

Protein sequence:

>791_residues
MKLNHRILLLIAPMILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLVSSYAFTLAKSDIIRHYFSLE
KNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELLYYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNS
AGEDILVRYDMLDTQTLSTPLSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENNQRKNIEKLNTDDEIGRLSSR
FYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLLLSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFA
THVRQLCKNFEASHNTYSIAARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQPYFTCSGKRLVGFEVLLRWQS
ELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQAIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAW
IDFEITETFAADSQSFPLLHELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHYCFTEPSQSECR

Sequences:

>Translated_791_residues
MKLNHRILLLIAPMILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLVSSYAFTLAKSDIIRHYFSLE
KNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELLYYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNS
AGEDILVRYDMLDTQTLSTPLSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENNQRKNIEKLNTDDEIGRLSSR
FYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLLLSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFA
THVRQLCKNFEASHNTYSIAARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQPYFTCSGKRLVGFEVLLRWQS
ELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQAIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAW
IDFEITETFAADSQSFPLLHELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHYCFTEPSQSECR
>Mature_791_residues
MKLNHRILLLIAPMILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLVSSYAFTLAKSDIIRHYFSLE
KNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELLYYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNS
AGEDILVRYDMLDTQTLSTPLSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENNQRKNIEKLNTDDEIGRLSSR
FYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLLLSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFA
THVRQLCKNFEASHNTYSIAARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQPYFTCSGKRLVGFEVLLRWQS
ELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQAIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAW
IDFEITETFAADSQSFPLLHELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHYCFTEPSQSECR

Specific function: Plays an essential role in the maintenance and the formation of the three-dimensional structure of the biofilms at the later stages of their development. Absence of mbaA promotes the accumulation of larger amount of biomass on the surfaces at later stage

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Single-pass type I membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=442, Percent_Identity=29.185520361991, Blast_Score=197, Evalue=3e-51,
Organism=Escherichia coli, GI87081921, Length=462, Percent_Identity=27.0562770562771, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1790496, Length=246, Percent_Identity=32.9268292682927, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI226510982, Length=653, Percent_Identity=23.1240428790199, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI87081743, Length=242, Percent_Identity=30.5785123966942, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788502, Length=248, Percent_Identity=25.8064516129032, Blast_Score=109, Evalue=9e-25,
Organism=Escherichia coli, GI1787055, Length=244, Percent_Identity=29.5081967213115, Blast_Score=103, Evalue=6e-23,
Organism=Escherichia coli, GI87081980, Length=254, Percent_Identity=28.3464566929134, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788849, Length=299, Percent_Identity=29.7658862876254, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI87081845, Length=250, Percent_Identity=25.6, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786507, Length=247, Percent_Identity=23.0769230769231, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1788381, Length=314, Percent_Identity=28.9808917197452, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082096, Length=200, Percent_Identity=28.5, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=195, Percent_Identity=31.7948717948718, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=32.3353293413174, Blast_Score=76, Evalue=9e-15,
Organism=Escherichia coli, GI1788085, Length=190, Percent_Identity=32.6315789473684, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787802, Length=140, Percent_Identity=30.7142857142857, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788956, Length=256, Percent_Identity=27.34375, Blast_Score=73, Evalue=9e-14,
Organism=Escherichia coli, GI1786584, Length=184, Percent_Identity=26.6304347826087, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=7e-13,
Organism=Escherichia coli, GI1787816, Length=142, Percent_Identity=28.8732394366197, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=158, Percent_Identity=32.2784810126582, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00672 HAMP [H]

EC number: NA

Molecular weight: Translated: 90828; Mature: 90828

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50885 HAMP ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNHRILLLIAPMILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLV
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHEEEHHHHHHHHHHHHHHH
SSYAFTLAKSDIIRHYFSLEKNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCEE
YYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNSAGEDILVRYDMLDTQTLSTP
EEECCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCHHHCCC
LSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHEECCCCEEE
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENN
EECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRKNIEKLNTDDEIGRLSSRFYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHHHH
LSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFATHVRQLCKNFEASHNTYSIA
HHHHCCCCEEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEECCCCHH
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQP
HHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECC
YFTCSGKRLVGFEVLLRWQSELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQ
EEEECCCEEHHHHHHHHHHHHHHCCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
AIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAWIDFEITETFAADSQSFPLLH
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCHHHH
ELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
HHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHY
CCCEEECCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCHHHCCHHHHCCCCCHHHHCCCCC
CFTEPSQSECR
CCCCCCCCCCC
>Mature Secondary Structure
MKLNHRILLLIAPMILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLV
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHEEEHHHHHHHHHHHHHHH
SSYAFTLAKSDIIRHYFSLEKNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCEE
YYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNSAGEDILVRYDMLDTQTLSTP
EEECCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCHHHCCC
LSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHEECCCCEEE
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENN
EECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRKNIEKLNTDDEIGRLSSRFYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHHHH
LSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFATHVRQLCKNFEASHNTYSIA
HHHHCCCCEEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEECCCCHH
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQP
HHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECC
YFTCSGKRLVGFEVLLRWQSELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQ
EEEECCCEEHHHHHHHHHHHHHHCCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
AIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAWIDFEITETFAADSQSFPLLH
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCHHHH
ELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
HHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHY
CCCEEECCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCHHHCCHHHHCCCCCHHHHCCCCC
CFTEPSQSECR
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]