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The map label for this gene is nspS [H]

Identifier: 147674245

GI number: 147674245

Start: 244383

End: 245462

Strand: Reverse

Name: nspS [H]

Synonym: VC0395_A0234

Alternate gene names: 147674245

Gene position: 245462-244383 (Counterclockwise)

Preceding gene: 147674498

Following gene: 147674861

Centisome position: 8.12

GC content: 46.85

Gene sequence:

>1080_bases
GTGACCAATTTTTGTAACGAATGGGTATCGTATTCACAAATGATCAAACGCTTTCTGTCGCTGATGGTATTGAATACTGT
CTGTTATCAAGCCAGCGCTTTGGAACTGAATGTCTACCTTTGGGAAGATACGATTGCACCAAGCGTAGTTGAGGCATGGC
ATAAAAAAACCGGCGTCTCCGTTAATCTCTACCATTTCGATAATGATGATGAACGCAGTTTATTAATGCTCAAAAGCGTA
CAACTGCCCTTTGACATCATGGTGTTGGATAACGTCTCCGCGTTCATTTTCTCAAGGCAAAATGTTTTTGAGGATCTGAC
CGCCCTTCCTAATCGTGCGAATAATGACCCTATGTGGCTACAAGCTTGTGGCACCCATGCGGTACCTTATTTCTGGGGCT
CAGTCGGCATTGCCTATCGCAAAAGCTTGTTTGATAAACCTCCAACGCAGTGGAGTGAGGTCGTCGATATCGCTCCGGCG
CATCGTGGACGAGTGGGTATGCTTAAAGACAGTGTCGAAACACTACTGCCTGCACTGTACATGCTCAACGCCTCTCCCAT
TACCGATTCTATCGACACACTAAGGCAAGCGTATCGTCTCTTAGACGCCGCCAATCCCCATATCCTGACCTATGAGTATG
TATTGAGCTATGTACGCAGCCACCCACAAACCGACAATCTACATATGGCGGTCTCTTACAGTGGCGATCACTATTCACTG
AACCGCTTCTTCAATACTCAAGACTGGGACTTTAGCGTTCCAGAAGGCCGCCCTTATTTGTGGGTTGATTGTATGGCCGT
AAATAGCGTTTCTCCCAACACAGTGCAGGCCAAAGCTTTTTTGGATTTCTTGATGAAACCTGACATTGCCGCAATCAATG
CCGAATATATTCGAGCTGCAAGCCCAAACTACAAAGCACGCGCTTTGCTCCCTGTAGAGCACCGAGAAGATCTCTCGATT
TATCTCCCAGAGCAGCGATTGGCGGAGGGAATAATCTACAGCGAACTCTCGGCGAAAAACCTCAGTCTGCGTGCCAAAAT
CATCAGCAGTGTGACTTACCAATATGAAGCTAAACCATAG

Upstream 100 bases:

>100_bases
CTGATTATAATGCGACCAAATTATACATAGAGTTAGGTGATCCAGGCTGTCAGCGTAGCCAAAATATTGGCTTTTACGCT
ATTAGGTTATTTGTCGGATC

Downstream 100 bases:

>100_bases
AATTCTGTTACTGATTGCACCGATGATCCTGCTCAGTGCAGCCGCATCAAGCTACATCATTTACACCAGTCAGAAAAATG
CGCTGCTAAAAAGAACCGAC

Product: putative spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: Norspermidine-binding protein [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVSVNLYHFDNDDERSLLMLKSV
QLPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWLQACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPA
HRGRVGMLKDSVETLLPALYMLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSL
NRFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAASPNYKARALLPVEHREDLSI
YLPEQRLAEGIIYSELSAKNLSLRAKIISSVTYQYEAKP

Sequences:

>Translated_359_residues
MTNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVSVNLYHFDNDDERSLLMLKSV
QLPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWLQACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPA
HRGRVGMLKDSVETLLPALYMLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSL
NRFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAASPNYKARALLPVEHREDLSI
YLPEQRLAEGIIYSELSAKNLSLRAKIISSVTYQYEAKP
>Mature_358_residues
TNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVSVNLYHFDNDDERSLLMLKSVQ
LPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWLQACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPAH
RGRVGMLKDSVETLLPALYMLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSLN
RFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAASPNYKARALLPVEHREDLSIY
LPEQRLAEGIIYSELSAKNLSLRAKIISSVTYQYEAKP

Specific function: Acts as a sensor of norspermidine and enhances biofilm formation. When complexed to norspermidine, could interact with the periplasmic portion of mbaA to regulate its enzymatic activity [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=330, Percent_Identity=27.8787878787879, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787367, Length=314, Percent_Identity=23.5668789808917, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40963; Mature: 40832

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCE
VNLYHFDNDDERSLLMLKSVQLPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWL
EEEEECCCCCCCCEEEEEECCCCEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCCEEE
QACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPAHRGRVGMLKDSVETLLPALY
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSL
HHCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEEEECCCEEEE
NRFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAA
EECCCCCCCCEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEECHHHHCCC
SPNYKARALLPVEHREDLSIYLPEQRLAEGIIYSELSAKNLSLRAKIISSVTYQYEAKP
CCCCCEEEEEECCCCCCCEEEECHHHHHCCHHHHHCCCCCHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
TNFCNEWVSYSQMIKRFLSLMVLNTVCYQASALELNVYLWEDTIAPSVVEAWHKKTGVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCE
VNLYHFDNDDERSLLMLKSVQLPFDIMVLDNVSAFIFSRQNVFEDLTALPNRANNDPMWL
EEEEECCCCCCCCEEEEEECCCCEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCCEEE
QACGTHAVPYFWGSVGIAYRKSLFDKPPTQWSEVVDIAPAHRGRVGMLKDSVETLLPALY
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MLNASPITDSIDTLRQAYRLLDAANPHILTYEYVLSYVRSHPQTDNLHMAVSYSGDHYSL
HHCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEEEECCCEEEE
NRFFNTQDWDFSVPEGRPYLWVDCMAVNSVSPNTVQAKAFLDFLMKPDIAAINAEYIRAA
EECCCCCCCCEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEECHHHHCCC
SPNYKARALLPVEHREDLSIYLPEQRLAEGIIYSELSAKNLSLRAKIISSVTYQYEAKP
CCCCCEEEEEECCCCCCCEEEECHHHHHCCHHHHHCCCCCHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]