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The map label for this gene is cheB-2

Identifier: 147674819

GI number: 147674819

Start: 1769557

End: 1770690

Strand: Reverse

Name: cheB-2

Synonym: VC0395_A1650

Alternate gene names: 147674819

Gene position: 1770690-1769557 (Counterclockwise)

Preceding gene: 147674329

Following gene: 147675595

Centisome position: 58.55

GC content: 48.85

Gene sequence:

>1134_bases
ATGGCGATTAAAGTATTAGTTGTTGATGATTCGAGTTTTTTCCGTCGTCGCGTGAGTGAAATCATCAACTCCGAATCGCG
CTTAGAAGTCATTGATGTTGCTGTAAATGGGAAAGAGGCCGTAGAAAAGGCCGCTCGACTCAAGCCAGATGTGATCACTA
TGGATATAGAAATGCCCGTCATGGACGGTATTTCCGCTGTCCGAGAAATCATGGCAAACAATCCCGTGCCCATTTTGATG
TTCTCTTCATTGACCCATGATGGCGCAAAAGCCACATTGGATGCACTGGATGCTGGCGCGTTAGACTTTTTACCTAAGAA
GTTTGAAGATATCGCGCGCAACCGGGATGAAGCCGTCACACTGTTACAGCAGCGTGTGCTTTCTATTGCATCGAAAAAGA
TGTTTCTACGTCGCCCTGCAGCTCCACGCCCTGCGCCTACAACATCAATAGCAGCGAGTTCGTCACTCAGCCAAGAACGA
GCGGCAGCTACCTCACCTTTGGGAAACCGACCCTCGACGGCGGTTTCAGCAGCCACTCGTTTTAAAGCTTCGGGTAAAAA
ATACCAGTTGACCGCAATAGGAACCTCAACAGGTGGACCTGTAGCCTTGCAAAAAATATTAACTAAGCTCCCCGTAAATT
ATCCACACCCCATAGTGTTGATTCAGCATATGCCAGCAACATTTACGGCTGCTTTTGCTAGCCGTCTCAATTCGCTATGC
AAAATTGAAGTTAAAGAAGCGGAAGATGGAGATATGCTGCGTCCGGGTGTTGCTTATTTAGCACCGGGTGGTAAGCAGAT
GATGCTGGATGGTCGACCCGGTGCGGCTCGCCTACGTATTATCGATGGTGGCGACCGCATGAACTATAAACCGTGTGTGG
ATGTTACTTTTGGCTCCGCAGCGAAAATCTTTGGTGACAAAGTACTGTCCATGATTTTGACCGGAATGGGTGCGGATGGA
CGTGAAGGTGCACGGATGCTGAAACAAGCAGGAGCCACCATTTGGGCACAAGATGAAGAAAGTTGTGTCGTGTATGGCAT
GCCACAAGCTGTCGCTAAAGCAGGAATTTCAACGGAAGATCTACCGCTGGAGAGAATCGCAGAGCGTATGTTGGTAGAAG
TCGGGCTCGCCTAA

Upstream 100 bases:

>100_bases
GCGATGGTCATATTGCTCTGATTTTGGATGTGCCGGATCTGCTCAAGCAGTATGCAGCTGCTTCTCGCGTCTGATTAGCT
GCACAACGATAAGGATAAAC

Downstream 100 bases:

>100_bases
GGAACCTCAATGATCGTTTGGAGTGTAGCAAACCAAAAAGGTGGGGTAGGAAAAACCACCACAACGATTACATTGGCAGG
CTTATTAAGCCAGCAGGGTA

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAIKVLVVDDSSFFRRRVSEIINSESRLEVIDVAVNGKEAVEKAARLKPDVITMDIEMPVMDGISAVREIMANNPVPILM
FSSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVTLLQQRVLSIASKKMFLRRPAAPRPAPTTSIAASSSLSQER
AAATSPLGNRPSTAVSAATRFKASGKKYQLTAIGTSTGGPVALQKILTKLPVNYPHPIVLIQHMPATFTAAFASRLNSLC
KIEVKEAEDGDMLRPGVAYLAPGGKQMMLDGRPGAARLRIIDGGDRMNYKPCVDVTFGSAAKIFGDKVLSMILTGMGADG
REGARMLKQAGATIWAQDEESCVVYGMPQAVAKAGISTEDLPLERIAERMLVEVGLA

Sequences:

>Translated_377_residues
MAIKVLVVDDSSFFRRRVSEIINSESRLEVIDVAVNGKEAVEKAARLKPDVITMDIEMPVMDGISAVREIMANNPVPILM
FSSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVTLLQQRVLSIASKKMFLRRPAAPRPAPTTSIAASSSLSQER
AAATSPLGNRPSTAVSAATRFKASGKKYQLTAIGTSTGGPVALQKILTKLPVNYPHPIVLIQHMPATFTAAFASRLNSLC
KIEVKEAEDGDMLRPGVAYLAPGGKQMMLDGRPGAARLRIIDGGDRMNYKPCVDVTFGSAAKIFGDKVLSMILTGMGADG
REGARMLKQAGATIWAQDEESCVVYGMPQAVAKAGISTEDLPLERIAERMLVEVGLA
>Mature_376_residues
AIKVLVVDDSSFFRRRVSEIINSESRLEVIDVAVNGKEAVEKAARLKPDVITMDIEMPVMDGISAVREIMANNPVPILMF
SSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVTLLQQRVLSIASKKMFLRRPAAPRPAPTTSIAASSSLSQERA
AATSPLGNRPSTAVSAATRFKASGKKYQLTAIGTSTGGPVALQKILTKLPVNYPHPIVLIQHMPATFTAAFASRLNSLCK
IEVKEAEDGDMLRPGVAYLAPGGKQMMLDGRPGAARLRIIDGGDRMNYKPCVDVTFGSAAKIFGDKVLSMILTGMGADGR
EGARMLKQAGATIWAQDEESCVVYGMPQAVAKAGISTEDLPLERIAERMLVEVGLA

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=372, Percent_Identity=37.9032258064516, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1788550, Length=136, Percent_Identity=32.3529411764706, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_VIBCH (Q9KQD8)

Other databases:

- EMBL:   AE003852
- PIR:   H82122
- RefSeq:   NP_231694.1
- ProteinModelPortal:   Q9KQD8
- SMR:   Q9KQD8
- GeneID:   2613442
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2062
- TIGR:   VC_2062
- HOGENOM:   HBG705324
- OMA:   KMAAELN
- ProtClustDB:   PRK00742
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 40379; Mature: 40248

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 196-196 ACT_SITE 223-223 ACT_SITE 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKVLVVDDSSFFRRRVSEIINSESRLEVIDVAVNGKEAVEKAARLKPDVITMDIEMPV
CEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCEEEEEECCCH
MDGISAVREIMANNPVPILMFSSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVT
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHH
LLQQRVLSIASKKMFLRRPAAPRPAPTTSIAASSSLSQERAAATSPLGNRPSTAVSAATR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FKASGKKYQLTAIGTSTGGPVALQKILTKLPVNYPHPIVLIQHMPATFTAAFASRLNSLC
HHCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHE
KIEVKEAEDGDMLRPGVAYLAPGGKQMMLDGRPGAARLRIIDGGDRMNYKPCVDVTFGSA
EEEEECCCCCCCCCCCHHEECCCCCEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCH
AKIFGDKVLSMILTGMGADGREGARMLKQAGATIWAQDEESCVVYGMPQAVAKAGISTED
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEECHHHHHHCCCCCCC
LPLERIAERMLVEVGLA
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIKVLVVDDSSFFRRRVSEIINSESRLEVIDVAVNGKEAVEKAARLKPDVITMDIEMPV
EEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCEEEEEECCCH
MDGISAVREIMANNPVPILMFSSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVT
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHH
LLQQRVLSIASKKMFLRRPAAPRPAPTTSIAASSSLSQERAAATSPLGNRPSTAVSAATR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FKASGKKYQLTAIGTSTGGPVALQKILTKLPVNYPHPIVLIQHMPATFTAAFASRLNSLC
HHCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHE
KIEVKEAEDGDMLRPGVAYLAPGGKQMMLDGRPGAARLRIIDGGDRMNYKPCVDVTFGSA
EEEEECCCCCCCCCCCHHEECCCCCEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCH
AKIFGDKVLSMILTGMGADGREGARMLKQAGATIWAQDEESCVVYGMPQAVAKAGISTED
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEECHHHHHHCCCCCCC
LPLERIAERMLVEVGLA
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301