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The map label for this gene is iaaA [H]

Identifier: 147674447

GI number: 147674447

Start: 2326656

End: 2327636

Strand: Reverse

Name: iaaA [H]

Synonym: VC0395_A2181

Alternate gene names: 147674447

Gene position: 2327636-2326656 (Counterclockwise)

Preceding gene: 147674781

Following gene: 147673842

Centisome position: 76.97

GC content: 52.5

Gene sequence:

>981_bases
ATGACACAGCCTTTTGCGATTGCCATCCATGGCGGTGCCGGCACGATTTTGCGTGAGCAGATGAGTGATGCATTGCGCAT
GGACATCCTCGCGACTTTAGAGCAAGCCGTGCGAGCCGGGCATCAGCTACTGGTTGAAGGAGCCGATGCCTTAGATGCGG
TGGTGGCAGCGGTGACAGTATTGGAAGACTCGCCACTGTTTAATGCCGGCAAAGGCTCAGTACTGACGCACAATGAAATG
GTGGAGATGGATGCCTCGGTGATGCATGGCGCAGCCCGAGAGGCTGGCGCGATTGCGGGTGTGCGTCATGTTCGCAACCC
AATTCAACTCGCGCGCGATGTGCTGCGTCATAGTGATCATGTGTTTTTGATTGGTGATGGGGCGGAGCAATTTGCTTTCC
TGCAAGGGCATGTTTATACCGAGCAAGACTATTTTTTCACTGAGCGCCGTTATGAACAGCTGCAAAACATGAAGCAGCAA
GATCGCTTTGCACTTTCAGAAGCTTCCTATCAATCAGAGTCTGCTGAACAAGAGCCGCCAAGTGAATATCCCGATGATAA
GAAATTTGGCACGGTAGGCGCGGTGGCGCTGGATCAACAGGGCAATTTGGCGGCGGCGACCAGTACGGGTGGGATCACCA
ATAAACGCTTTGGTCGCGTGGGCGACTCGCCAATCATCGGCGCTGGAACGCTGGCTGAAAATGGCAATGTCGCCATTTCA
TGCACTGGAATGGGCGAGTACTTCATCCGTTATGCGGTCGCAGGCGATATTGCAGCACGCATGCGTTATCTCAAAGAAGA
TGTCCATATCGCCTGTGAAACCGTGGTACAAGGTGAACTGAAAAGTGTAGGTGGTGAAGGGGGATTAATTGCGATTGATG
CGCAGGGCGATCTTCACTTTGCCATGAACAGTTCTGGCATGTATCGCGCGGGGATTGATCGCCATGGTCAATTCAGTGTG
AAAATTTACGCTGACGAATAA

Upstream 100 bases:

>100_bases
ACCGCGATCCGGTTAGCGCTTTTTTATTGCGAACTTTGGCACTATACGCCCATTCTAAACGCCGAAATGAAACGGTTAAC
CTTATTGGAGAACACAAGTC

Downstream 100 bases:

>100_bases
CCTTTTCTTAATAACTGAATGGATTTTCAAAGCCGTTTTATATGAATTTTTCATGCTTAAAAAATGACTGCAAGGATTCA
AATTCGGTGCTAGAATCCTT

Product: putative asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTQPFAIAIHGGAGTILREQMSDALRMDILATLEQAVRAGHQLLVEGADALDAVVAAVTVLEDSPLFNAGKGSVLTHNEM
VEMDASVMHGAAREAGAIAGVRHVRNPIQLARDVLRHSDHVFLIGDGAEQFAFLQGHVYTEQDYFFTERRYEQLQNMKQQ
DRFALSEASYQSESAEQEPPSEYPDDKKFGTVGAVALDQQGNLAAATSTGGITNKRFGRVGDSPIIGAGTLAENGNVAIS
CTGMGEYFIRYAVAGDIAARMRYLKEDVHIACETVVQGELKSVGGEGGLIAIDAQGDLHFAMNSSGMYRAGIDRHGQFSV
KIYADE

Sequences:

>Translated_326_residues
MTQPFAIAIHGGAGTILREQMSDALRMDILATLEQAVRAGHQLLVEGADALDAVVAAVTVLEDSPLFNAGKGSVLTHNEM
VEMDASVMHGAAREAGAIAGVRHVRNPIQLARDVLRHSDHVFLIGDGAEQFAFLQGHVYTEQDYFFTERRYEQLQNMKQQ
DRFALSEASYQSESAEQEPPSEYPDDKKFGTVGAVALDQQGNLAAATSTGGITNKRFGRVGDSPIIGAGTLAENGNVAIS
CTGMGEYFIRYAVAGDIAARMRYLKEDVHIACETVVQGELKSVGGEGGLIAIDAQGDLHFAMNSSGMYRAGIDRHGQFSV
KIYADE
>Mature_325_residues
TQPFAIAIHGGAGTILREQMSDALRMDILATLEQAVRAGHQLLVEGADALDAVVAAVTVLEDSPLFNAGKGSVLTHNEMV
EMDASVMHGAAREAGAIAGVRHVRNPIQLARDVLRHSDHVFLIGDGAEQFAFLQGHVYTEQDYFFTERRYEQLQNMKQQD
RFALSEASYQSESAEQEPPSEYPDDKKFGTVGAVALDQQGNLAAATSTGGITNKRFGRVGDSPIIGAGTLAENGNVAISC
TGMGEYFIRYAVAGDIAARMRYLKEDVHIACETVVQGELKSVGGEGGLIAIDAQGDLHFAMNSSGMYRAGIDRHGQFSVK
IYADE

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=316, Percent_Identity=39.2405063291139, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI145275200, Length=316, Percent_Identity=39.2405063291139, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI98991769, Length=298, Percent_Identity=29.8657718120805, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI285002251, Length=308, Percent_Identity=28.8961038961039, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI285002253, Length=308, Percent_Identity=26.9480519480519, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1787050, Length=304, Percent_Identity=49.671052631579, Blast_Score=273, Evalue=2e-74,
Organism=Drosophila melanogaster, GI18921183, Length=318, Percent_Identity=35.8490566037736, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=31.7164179104478, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24655553, Length=185, Percent_Identity=31.3513513513514, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24657358, Length=315, Percent_Identity=25.3968253968254, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 35054; Mature: 34923

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPFAIAIHGGAGTILREQMSDALRMDILATLEQAVRAGHQLLVEGADALDAVVAAVTV
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHH
LEDSPLFNAGKGSVLTHNEMVEMDASVMHGAAREAGAIAGVRHVRNPIQLARDVLRHSDH
HCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VFLIGDGAEQFAFLQGHVYTEQDYFFTERRYEQLQNMKQQDRFALSEASYQSESAEQEPP
EEEEECCHHHEEEEECEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SEYPDDKKFGTVGAVALDQQGNLAAATSTGGITNKRFGRVGDSPIIGAGTLAENGNVAIS
CCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCEECCCCCEEEE
CTGMGEYFIRYAVAGDIAARMRYLKEDVHIACETVVQGELKSVGGEGGLIAIDAQGDLHF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
AMNSSGMYRAGIDRHGQFSVKIYADE
EECCCCCEEECCCCCCEEEEEEEECC
>Mature Secondary Structure 
TQPFAIAIHGGAGTILREQMSDALRMDILATLEQAVRAGHQLLVEGADALDAVVAAVTV
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHH
LEDSPLFNAGKGSVLTHNEMVEMDASVMHGAAREAGAIAGVRHVRNPIQLARDVLRHSDH
HCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VFLIGDGAEQFAFLQGHVYTEQDYFFTERRYEQLQNMKQQDRFALSEASYQSESAEQEPP
EEEEECCHHHEEEEECEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SEYPDDKKFGTVGAVALDQQGNLAAATSTGGITNKRFGRVGDSPIIGAGTLAENGNVAIS
CCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCEECCCCCEEEE
CTGMGEYFIRYAVAGDIAARMRYLKEDVHIACETVVQGELKSVGGEGGLIAIDAQGDLHF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
AMNSSGMYRAGIDRHGQFSVKIYADE
EECCCCCEEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]