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The map label for this gene is slyD [H]

Identifier: 147674781

GI number: 147674781

Start: 2327799

End: 2328398

Strand: Reverse

Name: slyD [H]

Synonym: VC0395_A2182

Alternate gene names: 147674781

Gene position: 2328398-2327799 (Counterclockwise)

Preceding gene: 147675564

Following gene: 147674447

Centisome position: 77.0

GC content: 52.33

Gene sequence:

>600_bases
ATGAAAATTGAAAAAAACACGGTAGCAAGTCTTGCTTACCAATTGACTATCGAAGATGGCGTGGTGGTGGATCAGTCTAC
TGTTGATGCGCCGCTGGATTACCTGCACGGTCACAATAACCTGATTACAGGTCTTGAGCGTGAATTGGAAGGCAAAGTGG
CTGGTGATAAATTCACCGTTACTATCGCACCAGAAGATGCATACGGCGAACACAACGAAGATCTGGTACAGCGTGTTCCT
GCAGACGTGTTCCAAGGAGTGGATGAGCTGGAAGTCGGTATGCGTTTCCTCGCCGATACAGACCAAGGTCCAATCCCAGT
TGAAATCACTGAAGTGGATGGCGATGAAGTCGTGGTTGATGGTAACCACATGCTGGCTGGCCAGTCACTGACTTTCACCG
TAGACGTGGTTGCCGTGCGCGCAGCGACGGAAGATGAGATTGCTCACGGTCATATCCACCAAGTGGGTGGCTGTGGTCAC
GATCACGACCATGATCATGAAGGCGGTTGCTGCGGTGGCGAAGGCCACGGTCTCGATCACCATGGTCACGGTAAGAAAGA
AGGCGGCTGCTGTGGCGGCGGCGGTTGTGGTTCTCACTAA

Upstream 100 bases:

>100_bases
CCGGAGTAATTGAGTTTCTGCGTGTGATCTTCATAATATCGGCAACATTCTTCTCTTGGCCTGCATTTGGCAGTCAAGAG
ACAACCTCCCTGGAGCAGTT

Downstream 100 bases:

>100_bases
TAGTGAGCCCATCTCGCTAAGTAGTACGTTATTTTCGTAGTACGACAGATTTGAAAGTGCTAACCGCGATCCGGTTAGCG
CTTTTTTATTGCGAACTTTG

Product: peptidyl-prolyl cis-trans isomerase, FKBP-type

Products: NA

Alternate protein names: PPIase; Metallochaperone slyD [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKIEKNTVASLAYQLTIEDGVVVDQSTVDAPLDYLHGHNNLITGLERELEGKVAGDKFTVTIAPEDAYGEHNEDLVQRVP
ADVFQGVDELEVGMRFLADTDQGPIPVEITEVDGDEVVVDGNHMLAGQSLTFTVDVVAVRAATEDEIAHGHIHQVGGCGH
DHDHDHEGGCCGGEGHGLDHHGHGKKEGGCCGGGGCGSH

Sequences:

>Translated_199_residues
MKIEKNTVASLAYQLTIEDGVVVDQSTVDAPLDYLHGHNNLITGLERELEGKVAGDKFTVTIAPEDAYGEHNEDLVQRVP
ADVFQGVDELEVGMRFLADTDQGPIPVEITEVDGDEVVVDGNHMLAGQSLTFTVDVVAVRAATEDEIAHGHIHQVGGCGH
DHDHDHEGGCCGGEGHGLDHHGHGKKEGGCCGGGGCGSH
>Mature_199_residues
MKIEKNTVASLAYQLTIEDGVVVDQSTVDAPLDYLHGHNNLITGLERELEGKVAGDKFTVTIAPEDAYGEHNEDLVQRVP
ADVFQGVDELEVGMRFLADTDQGPIPVEITEVDGDEVVVDGNHMLAGQSLTFTVDVVAVRAATEDEIAHGHIHQVGGCGH
DHDHDHEGGCCGGEGHGLDHHGHGKKEGGCCGGGGCGSH

Specific function: Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity [H]

COG id: COG1047

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerases 2

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789748, Length=148, Percent_Identity=68.2432432432432, Blast_Score=206, Evalue=9e-55,
Organism=Escherichia coli, GI1786211, Length=146, Percent_Identity=32.1917808219178, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179 [H]

Pfam domain/function: PF00254 FKBP_C [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20986; Mature: 20986

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEKNTVASLAYQLTIEDGVVVDQSTVDAPLDYLHGHNNLITGLERELEGKVAGDKFTV
CCCCHHHHEEEEEEEEECCCEEEECCCCCCCHHHHCCCCCHHHHHHHHHCCEECCCEEEE
TIAPEDAYGEHNEDLVQRVPADVFQGVDELEVGMRFLADTDQGPIPVEITEVDGDEVVVD
EECCCCCCCCCCHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEC
GNHMLAGQSLTFTVDVVAVRAATEDEIAHGHIHQVGGCGHDHDHDHEGGCCGGEGHGLDH
CCEEEECCCEEEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCC
HGHGKKEGGCCGGGGCGSH
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIEKNTVASLAYQLTIEDGVVVDQSTVDAPLDYLHGHNNLITGLERELEGKVAGDKFTV
CCCCHHHHEEEEEEEEECCCEEEECCCCCCCHHHHCCCCCHHHHHHHHHCCEECCCEEEE
TIAPEDAYGEHNEDLVQRVPADVFQGVDELEVGMRFLADTDQGPIPVEITEVDGDEVVVD
EECCCCCCCCCCHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEC
GNHMLAGQSLTFTVDVVAVRAATEDEIAHGHIHQVGGCGHDHDHDHEGGCCGGEGHGLDH
CCEEEECCCEEEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCC
HGHGKKEGGCCGGGGCGSH
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]