Click here to switch to the map view.

The map label for this gene is ilvI [H]

Identifier: 147674433

GI number: 147674433

Start: 2215132

End: 2216853

Strand: Reverse

Name: ilvI [H]

Synonym: VC0395_A2059

Alternate gene names: 147674433

Gene position: 2216853-2215132 (Counterclockwise)

Preceding gene: 147675212

Following gene: 147674009

Centisome position: 73.31

GC content: 47.85

Gene sequence:

>1722_bases
ATGGAAATGCTATCTGGCGCAGAGATGATCGTCCAATCTCTGATCAATGAAGGTGTGGAGCAAATCTTCGGATATCCCGG
CGGTTCAGTACTCGACATCTACGATGCCCTTCACGAAAAAACAGACCAAATTAAACACGTTCTGGTTCGTCACGAACAAG
CCGCAACACATATGGCTGATGGCTATGCGCGCGCCACTGGAAAACCGGGCGTAGTGCTCGTCTGCTCAGGTCCGGGCGCC
ACCAATACGGTGACTGGGATTGCAACCGCCTATATGGACTCGATTCCGATGATTGTGATCTCCGGTAACGTAGCCACTAA
TCTTATTGGTAACGATGCATTTCAAGAGTGCGATATCGTCGGCGTGTCTCGTCCCATCGTTAAACACAGCTTTTTGGTGA
AAAAAGCCGAAGATATTCCTGAAACCATCAAAAAAGCGTTTTATATCGCGTCAACGGGGCGTCCTGGTCCTGTAGTGATT
GACGTGCCAAAAGATGTGATGAGCCCGCTTAATAAGCTGCCTTATGAGTATCCAGAAACCATTAAAATGCGCTCTTACAA
CCCAACGACGGCTGGCCACAAAGGACAGATCAAAAAAGGGTTACGCGCACTGCTCGATGCGAAAAAGCCAGTACTGTACG
TCGGTGGCGGGGCGATTATTGCTAATGCAGATCAGCAAATTCGCCAACTTGCAGAGGCTTTAAATCTGCCCGTGGTTAGT
ACTTTGATGGGATTGGGCGTTTTTCCTGGCACTCATAAAAACTCACTGGGTATGCTTGGCATGCATGGTGTGTATGAAGC
CAATATGGCGATGCACAATGCAGATCTGATTTTTGGGGTAGGTGTGCGCTTTGATGACCGTACGACTAACAACCTAGAGA
AGTACTGCCCCAACGCGAAAATCATGCATATCGATATTGACCCGTCATCGATTTCTAAAAACGTGAAAGTCGATCTGCCG
ATTGTTGGTTCTGCCGACCAAGTATTGGATGGCATGCTCAAGCTTCTGGAAGAGAGCGCTGAGCGCAATGATGCGGCAGC
ATTGGATAGTTGGTGGAATGAGATCCAAGTGTGGCGTGATCGTCAGTGTTTGGCTTATGAAACCTCAGCAGAACGGATTA
AGCCTCAGCAAGTGATTGAAACGCTATATAAAATCACGGAAGGTAAAGCAATTTTGGCCTCGGATGTGGGGCAGCACCAA
ATGTTTGCGGCACTCTACTATCCGTTTGATAAGCCGCGTCAGTGGATTAACTCTGGCGGGCTAGGCACCATGGGTTTTGG
TTTACCTGCGGGAATGGGCGTGAAATTCGCCATGCCAGAAGAAGAGGTACTGGTCGTGACCGGAGATGGCAGTATTCAGA
TGAATATTCAAGAGCTATCGACCGCACTGCAGTACGACATTCCAGTAAAAATCATTAACCTCAACAACCGTTTCCTTGGA
ATGGTGAAACAGTGGCAGGATATTATTTATCAAGGTCGTCACTCTAACTCATACATGAGTTCTGTGCCGGATTTTGCGGA
GATCGCAGAAGCTTATGGTCATGTTGGGATCCGTATTTCTCACCCTGATGAGCTGCAAGCTGGGCTGGAAAAAGCGTTAG
CGATGAAAGATCGTCTGGTGTTTGTCGACATTAATGTCGATGAAACTGAGCACGTTTACCCAATGCAGATCAAAGGCGAA
GGGATGGACAAAATGTGGTTGAGCAAAACGGAGAGAACCTAA

Upstream 100 bases:

>100_bases
AAAGCGGAGCGTTTTCGAATTAGGCTACAAATAAGCCCTAAACTATGTAAAACCAACCCTTTTGCCGACCGAGCACAAGG
TATGTTGGTAAGGAGACAAG

Downstream 100 bases:

>100_bases
GATGAGACACATTATTTCACTGCTATTGGAAAACCAACCGGGTGCGCTGTCTCGTGTTGTCGGGCTCTTTTCCCAGCGCG
GGTACAACATTGAAACGCTA

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MEMLSGAEMIVQSLINEGVEQIFGYPGGSVLDIYDALHEKTDQIKHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTVTGIATAYMDSIPMIVISGNVATNLIGNDAFQECDIVGVSRPIVKHSFLVKKAEDIPETIKKAFYIASTGRPGPVVI
DVPKDVMSPLNKLPYEYPETIKMRSYNPTTAGHKGQIKKGLRALLDAKKPVLYVGGGAIIANADQQIRQLAEALNLPVVS
TLMGLGVFPGTHKNSLGMLGMHGVYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAKIMHIDIDPSSISKNVKVDLP
IVGSADQVLDGMLKLLEESAERNDAAALDSWWNEIQVWRDRQCLAYETSAERIKPQQVIETLYKITEGKAILASDVGQHQ
MFAALYYPFDKPRQWINSGGLGTMGFGLPAGMGVKFAMPEEEVLVVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAEIAEAYGHVGIRISHPDELQAGLEKALAMKDRLVFVDINVDETEHVYPMQIKGE
GMDKMWLSKTERT

Sequences:

>Translated_573_residues
MEMLSGAEMIVQSLINEGVEQIFGYPGGSVLDIYDALHEKTDQIKHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTVTGIATAYMDSIPMIVISGNVATNLIGNDAFQECDIVGVSRPIVKHSFLVKKAEDIPETIKKAFYIASTGRPGPVVI
DVPKDVMSPLNKLPYEYPETIKMRSYNPTTAGHKGQIKKGLRALLDAKKPVLYVGGGAIIANADQQIRQLAEALNLPVVS
TLMGLGVFPGTHKNSLGMLGMHGVYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAKIMHIDIDPSSISKNVKVDLP
IVGSADQVLDGMLKLLEESAERNDAAALDSWWNEIQVWRDRQCLAYETSAERIKPQQVIETLYKITEGKAILASDVGQHQ
MFAALYYPFDKPRQWINSGGLGTMGFGLPAGMGVKFAMPEEEVLVVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAEIAEAYGHVGIRISHPDELQAGLEKALAMKDRLVFVDINVDETEHVYPMQIKGE
GMDKMWLSKTERT
>Mature_573_residues
MEMLSGAEMIVQSLINEGVEQIFGYPGGSVLDIYDALHEKTDQIKHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTVTGIATAYMDSIPMIVISGNVATNLIGNDAFQECDIVGVSRPIVKHSFLVKKAEDIPETIKKAFYIASTGRPGPVVI
DVPKDVMSPLNKLPYEYPETIKMRSYNPTTAGHKGQIKKGLRALLDAKKPVLYVGGGAIIANADQQIRQLAEALNLPVVS
TLMGLGVFPGTHKNSLGMLGMHGVYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAKIMHIDIDPSSISKNVKVDLP
IVGSADQVLDGMLKLLEESAERNDAAALDSWWNEIQVWRDRQCLAYETSAERIKPQQVIETLYKITEGKAILASDVGQHQ
MFAALYYPFDKPRQWINSGGLGTMGFGLPAGMGVKFAMPEEEVLVVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAEIAEAYGHVGIRISHPDELQAGLEKALAMKDRLVFVDINVDETEHVYPMQIKGE
GMDKMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=606, Percent_Identity=26.4026402640264, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI21361361, Length=511, Percent_Identity=26.027397260274, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=71.1304347826087, Blast_Score=894, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=41.9469026548673, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786717, Length=535, Percent_Identity=33.2710280373832, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=28.494623655914, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1788716, Length=591, Percent_Identity=23.5194585448393, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17531301, Length=561, Percent_Identity=27.6292335115865, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17531299, Length=561, Percent_Identity=27.6292335115865, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17542570, Length=576, Percent_Identity=26.0416666666667, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=42.5385934819897, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=496, Percent_Identity=25.4032258064516, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323163, Length=505, Percent_Identity=21.1881188118812, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=609, Percent_Identity=25.615763546798, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62923; Mature: 62923

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGAEMIVQSLINEGVEQIFGYPGGSVLDIYDALHEKTDQIKHVLVRHEQAATHMAD
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYARATGKPGVVLVCSGPGATNTVTGIATAYMDSIPMIVISGNVATNLIGNDAFQECDIV
CHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHCCCCHHHHCCEE
GVSRPIVKHSFLVKKAEDIPETIKKAFYIASTGRPGPVVIDVPKDVMSPLNKLPYEYPET
ECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHHHHHHCCCCCCCC
IKMRSYNPTTAGHKGQIKKGLRALLDAKKPVLYVGGGAIIANADQQIRQLAEALNLPVVS
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHHHHHHHCCCHHH
TLMGLGVFPGTHKNSLGMLGMHGVYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAK
HHHHCCCCCCCCCCCCCCCCCCCCEEHHHHEECCCEEEEECEEECCCCCCCHHHHCCCCE
IMHIDIDPSSISKNVKVDLPIVGSADQVLDGMLKLLEESAERNDAAALDSWWNEIQVWRD
EEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
RQCLAYETSAERIKPQQVIETLYKITEGKAILASDVGQHQMFAALYYPFDKPRQWINSGG
CCEEEEECCHHHCCHHHHHHHHHHHHCCCEEEECCCCHHHEEHHEECCCHHHHHHHCCCC
LGTMGFGLPAGMGVKFAMPEEEVLVVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
CCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEHHHHHHHHEECCCEEEEECCCHHHH
MVKQWQDIIYQGRHSNSYMSSVPDFAEIAEAYGHVGIRISHPDELQAGLEKALAMKDRLV
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCEE
FVDINVDETEHVYPMQIKGEGMDKMWLSKTERT
EEEECCCCCCCEEEEEECCCCCCHHHHHCCCCC
>Mature Secondary Structure
MEMLSGAEMIVQSLINEGVEQIFGYPGGSVLDIYDALHEKTDQIKHVLVRHEQAATHMAD
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYARATGKPGVVLVCSGPGATNTVTGIATAYMDSIPMIVISGNVATNLIGNDAFQECDIV
CHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHCCCCHHHHCCEE
GVSRPIVKHSFLVKKAEDIPETIKKAFYIASTGRPGPVVIDVPKDVMSPLNKLPYEYPET
ECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHHHHHHCCCCCCCC
IKMRSYNPTTAGHKGQIKKGLRALLDAKKPVLYVGGGAIIANADQQIRQLAEALNLPVVS
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHHHHHHHCCCHHH
TLMGLGVFPGTHKNSLGMLGMHGVYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAK
HHHHCCCCCCCCCCCCCCCCCCCCEEHHHHEECCCEEEEECEEECCCCCCCHHHHCCCCE
IMHIDIDPSSISKNVKVDLPIVGSADQVLDGMLKLLEESAERNDAAALDSWWNEIQVWRD
EEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
RQCLAYETSAERIKPQQVIETLYKITEGKAILASDVGQHQMFAALYYPFDKPRQWINSGG
CCEEEEECCHHHCCHHHHHHHHHHHHCCCEEEECCCCHHHEEHHEECCCHHHHHHHCCCC
LGTMGFGLPAGMGVKFAMPEEEVLVVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
CCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEHHHHHHHHEECCCEEEEECCCHHHH
MVKQWQDIIYQGRHSNSYMSSVPDFAEIAEAYGHVGIRISHPDELQAGLEKALAMKDRLV
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCEE
FVDINVDETEHVYPMQIKGEGMDKMWLSKTERT
EEEECCCCCCCEEEEEECCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]