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The map label for this gene is tilS

Identifier: 147674336

GI number: 147674336

Start: 1948599

End: 1949921

Strand: Reverse

Name: tilS

Synonym: VC0395_A1834

Alternate gene names: 147674336

Gene position: 1949921-1948599 (Counterclockwise)

Preceding gene: 147674788

Following gene: 147675046

Centisome position: 64.48

GC content: 52.38

Gene sequence:

>1323_bases
ATGGACGATCTCTATCTCCACTTTGTGCAGCAATTAGCTCGCTATCCTTTTCGCAAAATCCTTCTCGCACTGAGCGGCGG
CGTGGATTCGCAAGTTCTGCTTGCCCTTCTGGCGCGTGGACGCGATGAGTTTGGTTGGGATGTGACCGCGGTTCATGTTC
ATCATGGGTTGAGCCCGAATGCCGATCAGTGGGCGCAGCATTGCCAGCGCTGTTGCCGTGAAGTCGGCATGGCCTGTCAA
ATCGAATATGTACAGCTGGATGTGGCAAGTGGTGAAAGTATTGAGAAACTGGCGCGAGAGGCGCGTTATCGAGTCCTTGC
TCCGCATGTGAATGCGCAGACACTGTTGTTACTTGGGCAGCATGCTGACGATCAACTGGAAACTTTTTTACTCGCATTAA
AGCGCGGCAGTGGCCCAAAAGGGCTGGCCGCAATGGCTGCTTATGCCCCGTTTGCCGAGGGACATTTGCTCCGCCCTCTA
TTAACCGTCTCACGTCAACACATTGAAGCCTATGCCAAGCAGCACAAACTCACTTGGGTTATCGATGAAAGTAATGCAGA
TATCCGCTACGAGCGCAATTTTCTACGCCATCAGGTGACACCTGTACTTACCGAGCGCTGGCCGAGCATTCGCCAAGCTG
TGCAACGTAGTGCTGAGCTGTGTGCAGAACAAGAAGCGCTGCTGCAAGAGTTTTTAGCTGAAGCATTAAAAAAGGCGATC
ACTGCTGAGGGCGGATTGTCGATCGCCGTTCTGGCTGAGGGCAGTGAGGGAATGCGTCGACAACTGATCCGAGCTTGGTT
TGCGCACCATAGGCTACCGATGCCCAGTCGTCAGCACACGGAGCGTATTTGGTGTGAGGTGGCGCTGGCGAGTGAGGACG
CCAATCCAAAATTAAAGTTGAACCATATTGAGGTGCGCCGTTTTCAGCACTGTCTGTACCTCGTGCCACCAGAAAAAGAT
CTTTCAGGCTGGCGCAGTGCATTAGTGCCTGAACAACGTTTGCCGTTGCCGCAAGGTCTAGGTCATCTTCAGTTGACCAG
CAAGGCAGGTGGAAATATCAAGCTGCCCGATGACCCCAGCCAATTATGGGTTAGCTTTGAGCCGCAAGGGCTAGAGGCTT
GTCCTGTTGGGCGGGTGGGTTCGCGCAAACTCAAAAAACTGTTTCAAGAATATGGTGTGCCGAGTTGGCGGCGGCGGCAA
ACACCCATCCTCATGTACCAAAATAGAGTGGTCTGTGTCGCGGATTTATTCGTAGATCGTGATTGGAGTGGGCAAGATTG
TGAGTTGGTTTGGTTCAAATCTCATGATTCTGTGCCAAAATAA

Upstream 100 bases:

>100_bases
GGAATATCCACATATAGAAAGGGTTGGCTTAGGTCAGCCCTTTGGCTTTTCTGGGCTCAAGGGGTAAACTTGAGCCACTT
TTCTCAACTCTCTGTGGTTT

Downstream 100 bases:

>100_bases
ATTCATAACCACACATAATCTATAGGGATAATTACATGGGGAAAGCAATGAACAAAATGGTTATAGCCCTCGCTTTGGGT
GGCTGCGCTATGTCTGGTGC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MDDLYLHFVQQLARYPFRKILLALSGGVDSQVLLALLARGRDEFGWDVTAVHVHHGLSPNADQWAQHCQRCCREVGMACQ
IEYVQLDVASGESIEKLAREARYRVLAPHVNAQTLLLLGQHADDQLETFLLALKRGSGPKGLAAMAAYAPFAEGHLLRPL
LTVSRQHIEAYAKQHKLTWVIDESNADIRYERNFLRHQVTPVLTERWPSIRQAVQRSAELCAEQEALLQEFLAEALKKAI
TAEGGLSIAVLAEGSEGMRRQLIRAWFAHHRLPMPSRQHTERIWCEVALASEDANPKLKLNHIEVRRFQHCLYLVPPEKD
LSGWRSALVPEQRLPLPQGLGHLQLTSKAGGNIKLPDDPSQLWVSFEPQGLEACPVGRVGSRKLKKLFQEYGVPSWRRRQ
TPILMYQNRVVCVADLFVDRDWSGQDCELVWFKSHDSVPK

Sequences:

>Translated_440_residues
MDDLYLHFVQQLARYPFRKILLALSGGVDSQVLLALLARGRDEFGWDVTAVHVHHGLSPNADQWAQHCQRCCREVGMACQ
IEYVQLDVASGESIEKLAREARYRVLAPHVNAQTLLLLGQHADDQLETFLLALKRGSGPKGLAAMAAYAPFAEGHLLRPL
LTVSRQHIEAYAKQHKLTWVIDESNADIRYERNFLRHQVTPVLTERWPSIRQAVQRSAELCAEQEALLQEFLAEALKKAI
TAEGGLSIAVLAEGSEGMRRQLIRAWFAHHRLPMPSRQHTERIWCEVALASEDANPKLKLNHIEVRRFQHCLYLVPPEKD
LSGWRSALVPEQRLPLPQGLGHLQLTSKAGGNIKLPDDPSQLWVSFEPQGLEACPVGRVGSRKLKKLFQEYGVPSWRRRQ
TPILMYQNRVVCVADLFVDRDWSGQDCELVWFKSHDSVPK
>Mature_440_residues
MDDLYLHFVQQLARYPFRKILLALSGGVDSQVLLALLARGRDEFGWDVTAVHVHHGLSPNADQWAQHCQRCCREVGMACQ
IEYVQLDVASGESIEKLAREARYRVLAPHVNAQTLLLLGQHADDQLETFLLALKRGSGPKGLAAMAAYAPFAEGHLLRPL
LTVSRQHIEAYAKQHKLTWVIDESNADIRYERNFLRHQVTPVLTERWPSIRQAVQRSAELCAEQEALLQEFLAEALKKAI
TAEGGLSIAVLAEGSEGMRRQLIRAWFAHHRLPMPSRQHTERIWCEVALASEDANPKLKLNHIEVRRFQHCLYLVPPEKD
LSGWRSALVPEQRLPLPQGLGHLQLTSKAGGNIKLPDDPSQLWVSFEPQGLEACPVGRVGSRKLKKLFQEYGVPSWRRRQ
TPILMYQNRVVCVADLFVDRDWSGQDCELVWFKSHDSVPK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=409, Percent_Identity=46.6992665036675, Blast_Score=316, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_VIBC3 (A5F623)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F623
- SMR:   A5F623
- STRING:   A5F623
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1834
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   KSAVGFF
- ProtClustDB:   CLSK874732
- BioCyc:   VCHO345073:VC0395_A1834-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 49914; Mature: 49914

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLYLHFVQQLARYPFRKILLALSGGVDSQVLLALLARGRDEFGWDVTAVHVHHGLSPN
CCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
ADQWAQHCQRCCREVGMACQIEYVQLDVASGESIEKLAREARYRVLAPHVNAQTLLLLGQ
HHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHCCEEECCCCCCEEEEEEEC
HADDQLETFLLALKRGSGPKGLAAMAAYAPFAEGHLLRPLLTVSRQHIEAYAKQHKLTWV
CCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEE
IDESNADIRYERNFLRHQVTPVLTERWPSIRQAVQRSAELCAEQEALLQEFLAEALKKAI
EECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAEGGLSIAVLAEGSEGMRRQLIRAWFAHHRLPMPSRQHTERIWCEVALASEDANPKLKL
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECCCCCCCEEE
NHIEVRRFQHCLYLVPPEKDLSGWRSALVPEQRLPLPQGLGHLQLTSKAGGNIKLPDDPS
EHHHHHHHCCEEEEECCCCHHHHHHHHCCCHHCCCCCCCCCCEEEECCCCCEEECCCCHH
QLWVSFEPQGLEACPVGRVGSRKLKKLFQEYGVPSWRRRQTPILMYQNRVVCVADLFVDR
HEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCEEEECCCEEEEEEEEECC
DWSGQDCELVWFKSHDSVPK
CCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MDDLYLHFVQQLARYPFRKILLALSGGVDSQVLLALLARGRDEFGWDVTAVHVHHGLSPN
CCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
ADQWAQHCQRCCREVGMACQIEYVQLDVASGESIEKLAREARYRVLAPHVNAQTLLLLGQ
HHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHCCEEECCCCCCEEEEEEEC
HADDQLETFLLALKRGSGPKGLAAMAAYAPFAEGHLLRPLLTVSRQHIEAYAKQHKLTWV
CCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEE
IDESNADIRYERNFLRHQVTPVLTERWPSIRQAVQRSAELCAEQEALLQEFLAEALKKAI
EECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAEGGLSIAVLAEGSEGMRRQLIRAWFAHHRLPMPSRQHTERIWCEVALASEDANPKLKL
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECCCCCCCEEE
NHIEVRRFQHCLYLVPPEKDLSGWRSALVPEQRLPLPQGLGHLQLTSKAGGNIKLPDDPS
EHHHHHHHCCEEEEECCCCHHHHHHHHCCCHHCCCCCCCCCCEEEECCCCCEEECCCCHH
QLWVSFEPQGLEACPVGRVGSRKLKKLFQEYGVPSWRRRQTPILMYQNRVVCVADLFVDR
HEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCEEEECCCEEEEEEEEECC
DWSGQDCELVWFKSHDSVPK
CCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA