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The map label for this gene is hslV

Identifier: 147674179

GI number: 147674179

Start: 2396494

End: 2397051

Strand: Reverse

Name: hslV

Synonym: VC0395_A2248

Alternate gene names: 147674179

Gene position: 2397051-2396494 (Counterclockwise)

Preceding gene: 147673934

Following gene: 147673421

Centisome position: 79.27

GC content: 51.43

Gene sequence:

>558_bases
GTGACTACTATCGTATCAGTGCGTCGTAATAATAAAGTTGTCATCGCTGGTGACGGGCAGGTATCGCTAGGCAATACCGT
GATGAAAGGCAATGCACGTAAAGTGCGCCGCCTTTATAACAACAAGGTACTGGCAGGCTTTGCTGGTGGAACCGCTGATG
CATTTACCCTGTTTGAGCGCTTTGAAAGTAAGTTGCAGATGCACCAAGGTCACCTGACCAAAGCGGCGGTTGAGCTAGCC
AAAGATTGGCGAAGCGATCGCGCGCTGCGCCGTTTGGAAGCCATTTTGGCGGTCGCCGATGAAACCGCATCGCTGATCAT
TACCGGTAATGGTGATGTGCTGCAGCCTGAACACGATCTGATTGCGATCGGTTCTGGTGGCAACTATGCACAAGCGGCTG
CTATTGCTCTGTTAGAAAACACCGAGCTTGATGCACGCACGATCGCCGAAAAAGCCTTAAATATCGCGGGCGATATCTGC
GTGTTTACTAACCACCATCACACTATCGAAGAACTCGAAATTCCGCAGGCGATGTTGCCACAAGGCGCATCTGCGTAG

Upstream 100 bases:

>100_bases
TTGTTGGCATTCGGTCAACTTCTCTCGCTTGGCTTGAAATTGTTTTCCGGTAGCCGCATATAAGCGGGTAACTCTCCGAT
AAAAATTAAAGAGGCCTCTT

Downstream 100 bases:

>100_bases
ATCAATAAGTCTCCGGCAAATAACCACAAGATAGTGCCCGAAAAGCTGAGTGAAGGATTTGAATCATGTCTGAAATGACT
CCCCGCGAAATAGTGAGCGA

Product: ATP-dependent protease peptidase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTTIVSVRRNNKVVIAGDGQVSLGNTVMKGNARKVRRLYNNKVLAGFAGGTADAFTLFERFESKLQMHQGHLTKAAVELA
KDWRSDRALRRLEAILAVADETASLIITGNGDVLQPEHDLIAIGSGGNYAQAAAIALLENTELDARTIAEKALNIAGDIC
VFTNHHHTIEELEIPQAMLPQGASA

Sequences:

>Translated_185_residues
MTTIVSVRRNNKVVIAGDGQVSLGNTVMKGNARKVRRLYNNKVLAGFAGGTADAFTLFERFESKLQMHQGHLTKAAVELA
KDWRSDRALRRLEAILAVADETASLIITGNGDVLQPEHDLIAIGSGGNYAQAAAIALLENTELDARTIAEKALNIAGDIC
VFTNHHHTIEELEIPQAMLPQGASA
>Mature_184_residues
TTIVSVRRNNKVVIAGDGQVSLGNTVMKGNARKVRRLYNNKVLAGFAGGTADAFTLFERFESKLQMHQGHLTKAAVELAK
DWRSDRALRRLEAILAVADETASLIITGNGDVLQPEHDLIAIGSGGNYAQAAAIALLENTELDARTIAEKALNIAGDICV
FTNHHHTIEELEIPQAMLPQGASA

Specific function: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery

COG id: COG5405

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), peptidase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase T1B family. HslV subfamily

Homologues:

Organism=Escherichia coli, GI1790367, Length=172, Percent_Identity=84.8837209302326, Blast_Score=300, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLV_VIBC3 (A5F4X4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4X4
- SMR:   A5F4X4
- STRING:   A5F4X4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2248
- eggNOG:   COG5405
- HOGENOM:   HBG288822
- OMA:   AADICVY
- ProtClustDB:   PRK05456
- BioCyc:   VCHO345073:VC0395_A2248-MONOMER
- HAMAP:   MF_00248
- InterPro:   IPR022281
- InterPro:   IPR001353
- TIGRFAMs:   TIGR03692

Pfam domain/function: PF00227 Proteasome

EC number: 3.4.25.-

Molecular weight: Translated: 19898; Mature: 19767

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIVSVRRNNKVVIAGDGQVSLGNTVMKGNARKVRRLYNNKVLAGFAGGTADAFTLFER
CCEEEEEECCCEEEEEECCCEEECCEEECCCHHHHHHHHCCCEEEEECCCCHHHHHHHHH
FESKLQMHQGHLTKAAVELAKDWRSDRALRRLEAILAVADETASLIITGNGDVLQPEHDL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCE
IAIGSGGNYAQAAAIALLENTELDARTIAEKALNIAGDICVFTNHHHTIEELEIPQAMLP
EEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCHHHCC
QGASA
CCCCC
>Mature Secondary Structure 
TTIVSVRRNNKVVIAGDGQVSLGNTVMKGNARKVRRLYNNKVLAGFAGGTADAFTLFER
CEEEEEECCCEEEEEECCCEEECCEEECCCHHHHHHHHCCCEEEEECCCCHHHHHHHHH
FESKLQMHQGHLTKAAVELAKDWRSDRALRRLEAILAVADETASLIITGNGDVLQPEHDL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCE
IAIGSGGNYAQAAAIALLENTELDARTIAEKALNIAGDICVFTNHHHTIEELEIPQAMLP
EEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCHHHCC
QGASA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA