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The map label for this gene is hslU

Identifier: 147673421

GI number: 147673421

Start: 2395097

End: 2396428

Strand: Reverse

Name: hslU

Synonym: VC0395_A2247

Alternate gene names: 147673421

Gene position: 2396428-2395097 (Counterclockwise)

Preceding gene: 147674179

Following gene: 147675764

Centisome position: 79.25

GC content: 50.38

Gene sequence:

>1332_bases
ATGTCTGAAATGACTCCCCGCGAAATAGTGAGCGAGCTCAATCGCCACATCATAGGTCAAGATAAAGCCAAACGTGCCGT
AGCGATTGCACTGCGTAACCGTTGGCGTCGTATGCAGCTTGAAGAGAGCTTGCGTGTTGAAGTGACCCCGAAAAATATTC
TGATGATTGGCCCAACCGGTGTCGGCAAAACTGAAATTGCTCGCCGTTTAGCCAAGCTTGCGAACGCGCCTTTCATCAAG
GTTGAAGCGACCAAATTCACCGAAGTGGGCTATGTCGGTAAGGAAGTGGAATCGATCATCCGCGATCTGACTGATGTTGC
GGTGAAGCTGACGCATCAGCAAGCGATGGAGAAAGTAAAATTCCGCGCTGAAGAGTTAGCGGAAGAGCGCGTATTGGATG
CGCTGCTGCCTCCACCACGCGATGCGTGGGGACAAGCAGAGCAAAAAGAGGAGAACTCCAGCACCCGCCAAGTATTTCGT
AAAAAGTTGCGTGAAGGGCAACTGAACGACAAAGAGATTGAAATTAACGTCGCTGTACCGCAGATGGGCGTGGAAATCAT
GGCACCTCCAGGCATGGAAGAGATGACCAACCAACTGCAGGGACTGTTCCAAAACCTCGCGGGCGACACCAAGAAAAAGC
GCAAAATGAAGATTAAAGATGCGCTGAAAGCCTTGGTGGAAGAAGAAGCCGCTAAGCTAGTCAATCAAGAAGAGCTGAAA
GAGCAGGCGATTTACAATGTCGAAAATAACGGCATCGTGTTTATCGATGAAATCGACAAAATCTGTAAGCGTGGAGAAGT
CTCGGGTCCCGATGTTTCTCGTGAAGGGGTGCAGCGCGATCTGCTGCCTTTGATTGAAGGCAGTACGGTATCCACCAAGC
ATGGCATGGTGCGAACGGATCACATCCTGTTTATTGCCTCAGGCGCGTTCCAAGTAGCAAAACCGTCAGATTTGATCCCA
GAGCTGCAAGGCCGTTTGCCCATCCGTGTTGAGTTGGAAGCCCTGTCGAGCAACGACTTCAAACGCATTCTGACTGAGCC
GAAAGCATCACTCACCGAGCAATATGTTGCGCTGATGAAAACCGAACAGGTGGATGTGCAGTTTACCGAAGATGGTATCA
AGCAGATTGCGGATGCGGCATGGCAAGTTAACGAAACCACCGAAAATATTGGTGCGCGACGTCTGCATACTGTGCTGGAG
CGTTTAATGGATGAAATCTCCTTTGATGCAACAGAAAAAGCCGGCCAAGCCTTCGTGATCGATGCTGCTTACGTGAAAGC
GCGTTTGGGTGAGCTGGTCGAAGACGAAGATCTCAGCCGTTTCATTCTCTAG

Upstream 100 bases:

>100_bases
CGCAGGCGATGTTGCCACAAGGCGCATCTGCGTAGATCAATAAGTCTCCGGCAAATAACCACAAGATAGTGCCCGAAAAG
CTGAGTGAAGGATTTGAATC

Downstream 100 bases:

>100_bases
ACTGCTTAATTTTTGAAGTGAAAGCCCACCCGAATTTGGTGGGCTTTTTTATCTGGGTGATTTGCGCGTATACTGACGCC
ATGTTGATCGTACTCTCATT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSEMTPREIVSELNRHIIGQDKAKRAVAIALRNRWRRMQLEESLRVEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEKVKFRAEELAEERVLDALLPPPRDAWGQAEQKEENSSTRQVFR
KKLREGQLNDKEIEINVAVPQMGVEIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELK
EQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTDHILFIASGAFQVAKPSDLIP
ELQGRLPIRVELEALSSNDFKRILTEPKASLTEQYVALMKTEQVDVQFTEDGIKQIADAAWQVNETTENIGARRLHTVLE
RLMDEISFDATEKAGQAFVIDAAYVKARLGELVEDEDLSRFIL

Sequences:

>Translated_443_residues
MSEMTPREIVSELNRHIIGQDKAKRAVAIALRNRWRRMQLEESLRVEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEKVKFRAEELAEERVLDALLPPPRDAWGQAEQKEENSSTRQVFR
KKLREGQLNDKEIEINVAVPQMGVEIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELK
EQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTDHILFIASGAFQVAKPSDLIP
ELQGRLPIRVELEALSSNDFKRILTEPKASLTEQYVALMKTEQVDVQFTEDGIKQIADAAWQVNETTENIGARRLHTVLE
RLMDEISFDATEKAGQAFVIDAAYVKARLGELVEDEDLSRFIL
>Mature_442_residues
SEMTPREIVSELNRHIIGQDKAKRAVAIALRNRWRRMQLEESLRVEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEKVKFRAEELAEERVLDALLPPPRDAWGQAEQKEENSSTRQVFRK
KLREGQLNDKEIEINVAVPQMGVEIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELKE
QAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTDHILFIASGAFQVAKPSDLIPE
LQGRLPIRVELEALSSNDFKRILTEPKASLTEQYVALMKTEQVDVQFTEDGIKQIADAAWQVNETTENIGARRLHTVLER
LMDEISFDATEKAGQAFVIDAAYVKARLGELVEDEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=443, Percent_Identity=78.3295711060948, Blast_Score=716, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=100, Percent_Identity=47, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=124, Percent_Identity=33.8709677419355, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648291, Length=262, Percent_Identity=30.9160305343511, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648289, Length=262, Percent_Identity=30.9160305343511, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_VIBC3 (A5F4X3)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4X3
- SMR:   A5F4X3
- STRING:   A5F4X3
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2247
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   VCHO345073:VC0395_A2247-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 49873; Mature: 49742

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 256-256 BINDING 321-321 BINDING 393-393

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPREIVSELNRHIIGQDKAKRAVAIALRNRWRRMQLEESLRVEVTPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEKVK
CCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAEELAEERVLDALLPPPRDAWGQAEQKEENSSTRQVFRKKLREGQLNDKEIEINVAVP
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
QMGVEIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELK
CCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
EQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTD
HHHHHEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEC
HILFIASGAFQVAKPSDLIPELQGRLPIRVELEALSSNDFKRILTEPKASLTEQYVALMK
EEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHH
TEQVDVQFTEDGIKQIADAAWQVNETTENIGARRLHTVLERLMDEISFDATEKAGQAFVI
HCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEE
DAAYVKARLGELVEDEDLSRFIL
HHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SEMTPREIVSELNRHIIGQDKAKRAVAIALRNRWRRMQLEESLRVEVTPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEKVK
CCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAEELAEERVLDALLPPPRDAWGQAEQKEENSSTRQVFRKKLREGQLNDKEIEINVAVP
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
QMGVEIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELK
CCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
EQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTD
HHHHHEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEC
HILFIASGAFQVAKPSDLIPELQGRLPIRVELEALSSNDFKRILTEPKASLTEQYVALMK
EEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHH
TEQVDVQFTEDGIKQIADAAWQVNETTENIGARRLHTVLERLMDEISFDATEKAGQAFVI
HCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEE
DAAYVKARLGELVEDEDLSRFIL
HHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA