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The map label for this gene is fadA [H]

Identifier: 147674109

GI number: 147674109

Start: 2684792

End: 2685955

Strand: Reverse

Name: fadA [H]

Synonym: VC0395_A2533

Alternate gene names: 147674109

Gene position: 2685955-2684792 (Counterclockwise)

Preceding gene: 147673120

Following gene: 162329622

Centisome position: 88.82

GC content: 53.35

Gene sequence:

>1164_bases
ATGAATACAGTAGTGATTGTTGATTGCCTACGTACTCCGATGGGACGCTCCAAAGGCGGTGCGTTTCGCCACCAACGTGC
GGAAGATCTTTCAGCACATTTGATGAAAGGCATTCTGGCGCGCAACCCACAGGTCAATCCAAAAGAGATTGAAGACATTT
ATTGGGGCTGTGTGCAGCAAACCCTTGAACAAGGCTTTAACGTTGCGCGTAACGCAGCGCTGCTTGCGGGTCTGCCAATT
GAGATTGGCGCGGTCACCGTCAACCGTTTGTGTGGATCGTCCATGCAAGCACTGCATGATGCGGCGCGCGCAATCATGGT
TGGCGATGCAGAGATCTGTTTAGTGGGAGGCGTTGAGCACATGGGCCACGTGCCAATGACTCACGGCGTTGATTTCCATC
CGGGCCTGTCTAAGAACGTGGCCAAAGCAGCGGGAATGATGGGACTGACCGCAGAGATGCTCGGCAAGCTGCATGGTATT
AGCCGTCAGCAGCAAGATGAGTTCGCTGCGCGCTCTCATGCGCGTGCTCATGCAGCAACATTAGAAGGTCGCTTTAAAAA
CGAGATTCTGCCCACCGAGGGGCATGCTGCCGATGGTACGCTGTTTACCCTAGATTACGATGAAGTGATCCGCCCGGAAA
CCACAGTGGCGGGATTAGCTGAACTGCGTCCCGTATTCGATCCGGCCAACGGAACTGTGACTGCAGGAACCTCTTCGGCA
CTTTCGGATGGTGCTTCAGCGATGTTGGTGATGAGTGAACAGAAAGCCAAAGCACTCGGTTTAACCATTCGCGCTCGCAT
TAAAGCGATGGCCGTGGCCGGTTGCGATCCTTCGATCATGGGTTACGGCCCAGTACCTGCGACCCATAAAGCGCTCAAAC
GTGCGGGTTTAACCATGCAGGATATGGATGTGGTGGAATTGAACGAAGCGTTTGCCGCGCAATCTCTACCTTGCGCTAAA
GACTTAGGCTTACTGGATATGATGGATGACAAGGTCAACCTCAATGGCGGCGCGATAGCGCTCGGTCATCCACTGGGTTG
CTCGGGTACACGTATCTCAACCACGCTGATCAATTTGATGGAAGCCAAAGATGCGAAATATGGCCTTGCTACCATGTGTA
TCGGCTTAGGTCAGGGTATCGCGACCATTTTTGAGCGCCCATAA

Upstream 100 bases:

>100_bases
GTACCGCAACTGCTGAAGAACATGGCGCAACAAGGAACATCTTTCTACAGCGCTCAGCAAGCCAGTGCCCTGTAAATCGG
TTTAGAAAAGGAATAACAAA

Downstream 100 bases:

>100_bases
CCCTTCCGTTTTGTCTATCACTGAAGCGTCGTTTAAGAGCCAGCAACGCTGGCTCTTGTTATTAGGTTATTTAGCTTGTA
GCCAATCACTGTAAAGTTGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MNTVVIVDCLRTPMGRSKGGAFRHQRAEDLSAHLMKGILARNPQVNPKEIEDIYWGCVQQTLEQGFNVARNAALLAGLPI
EIGAVTVNRLCGSSMQALHDAARAIMVGDAEICLVGGVEHMGHVPMTHGVDFHPGLSKNVAKAAGMMGLTAEMLGKLHGI
SRQQQDEFAARSHARAHAATLEGRFKNEILPTEGHAADGTLFTLDYDEVIRPETTVAGLAELRPVFDPANGTVTAGTSSA
LSDGASAMLVMSEQKAKALGLTIRARIKAMAVAGCDPSIMGYGPVPATHKALKRAGLTMQDMDVVELNEAFAAQSLPCAK
DLGLLDMMDDKVNLNGGAIALGHPLGCSGTRISTTLINLMEAKDAKYGLATMCIGLGQGIATIFERP

Sequences:

>Translated_387_residues
MNTVVIVDCLRTPMGRSKGGAFRHQRAEDLSAHLMKGILARNPQVNPKEIEDIYWGCVQQTLEQGFNVARNAALLAGLPI
EIGAVTVNRLCGSSMQALHDAARAIMVGDAEICLVGGVEHMGHVPMTHGVDFHPGLSKNVAKAAGMMGLTAEMLGKLHGI
SRQQQDEFAARSHARAHAATLEGRFKNEILPTEGHAADGTLFTLDYDEVIRPETTVAGLAELRPVFDPANGTVTAGTSSA
LSDGASAMLVMSEQKAKALGLTIRARIKAMAVAGCDPSIMGYGPVPATHKALKRAGLTMQDMDVVELNEAFAAQSLPCAK
DLGLLDMMDDKVNLNGGAIALGHPLGCSGTRISTTLINLMEAKDAKYGLATMCIGLGQGIATIFERP
>Mature_387_residues
MNTVVIVDCLRTPMGRSKGGAFRHQRAEDLSAHLMKGILARNPQVNPKEIEDIYWGCVQQTLEQGFNVARNAALLAGLPI
EIGAVTVNRLCGSSMQALHDAARAIMVGDAEICLVGGVEHMGHVPMTHGVDFHPGLSKNVAKAAGMMGLTAEMLGKLHGI
SRQQQDEFAARSHARAHAATLEGRFKNEILPTEGHAADGTLFTLDYDEVIRPETTVAGLAELRPVFDPANGTVTAGTSSA
LSDGASAMLVMSEQKAKALGLTIRARIKAMAVAGCDPSIMGYGPVPATHKALKRAGLTMQDMDVVELNEAFAAQSLPCAK
DLGLLDMMDDKVNLNGGAIALGHPLGCSGTRISTTLINLMEAKDAKYGLATMCIGLGQGIATIFERP

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=41.0126582278481, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=39.9491094147583, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI148539872, Length=401, Percent_Identity=36.6583541147132, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=34.5088161209068, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI4504327, Length=432, Percent_Identity=34.4907407407407, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI194353979, Length=239, Percent_Identity=31.7991631799163, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI48994986, Length=386, Percent_Identity=76.1658031088083, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=45.9259259259259, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.3940149625935, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=42.75, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1788683, Length=428, Percent_Identity=32.2429906542056, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=40.2010050251256, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17535921, Length=397, Percent_Identity=35.0125944584383, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=433, Percent_Identity=31.1778290993072, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25147385, Length=392, Percent_Identity=31.8877551020408, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=387, Percent_Identity=25.8397932816537, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=38.8746803069054, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=35.3233830845771, Blast_Score=199, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=39.5, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI17648125, Length=334, Percent_Identity=44.0119760479042, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=33.0232558139535, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24640423, Length=401, Percent_Identity=33.9152119700748, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40829; Mature: 40829

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVVIVDCLRTPMGRSKGGAFRHQRAEDLSAHLMKGILARNPQVNPKEIEDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNVARNAALLAGLPIEIGAVTVNRLCGSSMQALHDAARAIMVGDAEICLVGGVEH
HHHHHHHHHHCCHHEECCCEEEHHHHHHHHHCHHHHHHHHHHHEEEECCCCEEEECCHHH
MGHVPMTHGVDFHPGLSKNVAKAAGMMGLTAEMLGKLHGISRQQQDEFAARSHARAHAAT
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LEGRFKNEILPTEGHAADGTLFTLDYDEVIRPETTVAGLAELRPVFDPANGTVTAGTSSA
HCCHHHCCCCCCCCCCCCCEEEEECHHHHHCCCHHHHHHHHHHHCCCCCCCEEECCCCHH
LSDGASAMLVMSEQKAKALGLTIRARIKAMAVAGCDPSIMGYGPVPATHKALKRAGLTMQ
HHCCCCEEEEEECCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCC
DMDVVELNEAFAAQSLPCAKDLGLLDMMDDKVNLNGGAIALGHPLGCSGTRISTTLINLM
CCCHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EAKDAKYGLATMCIGLGQGIATIFERP
HHCCCCHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MNTVVIVDCLRTPMGRSKGGAFRHQRAEDLSAHLMKGILARNPQVNPKEIEDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNVARNAALLAGLPIEIGAVTVNRLCGSSMQALHDAARAIMVGDAEICLVGGVEH
HHHHHHHHHHCCHHEECCCEEEHHHHHHHHHCHHHHHHHHHHHEEEECCCCEEEECCHHH
MGHVPMTHGVDFHPGLSKNVAKAAGMMGLTAEMLGKLHGISRQQQDEFAARSHARAHAAT
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LEGRFKNEILPTEGHAADGTLFTLDYDEVIRPETTVAGLAELRPVFDPANGTVTAGTSSA
HCCHHHCCCCCCCCCCCCCEEEEECHHHHHCCCHHHHHHHHHHHCCCCCCCEEECCCCHH
LSDGASAMLVMSEQKAKALGLTIRARIKAMAVAGCDPSIMGYGPVPATHKALKRAGLTMQ
HHCCCCEEEEEECCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCC
DMDVVELNEAFAAQSLPCAKDLGLLDMMDDKVNLNGGAIALGHPLGCSGTRISTTLINLM
CCCHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EAKDAKYGLATMCIGLGQGIATIFERP
HHCCCCHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]