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The map label for this gene is hupR1 [H]

Identifier: 147674084

GI number: 147674084

Start: 644977

End: 646320

Strand: Direct

Name: hupR1 [H]

Synonym: VC0395_A0606

Alternate gene names: 147674084

Gene position: 644977-646320 (Clockwise)

Preceding gene: 147674873

Following gene: 147673391

Centisome position: 21.33

GC content: 45.91

Gene sequence:

>1344_bases
ATGCAAATGGATAACATGCACGATGAGAAGTTAAACGTCCTGTTACTTGATGATGAAAACGATATTTTGAAAGCGCTTAA
CCGTGTGTTGCGGATGGATTACAACGTAGTGACCTTCGATAACGGCGCTGATGCGTTGGAATATCTGCAGGAAAACCCAA
TCCACATCATCATCTCGGATATGCGTATGCCAGAGATGGATGGTGCAGATTTTCTTGCCAAAGCGCGAGAAATGCAGCCG
GATACGGTGCGTTTGCTGCTCACCGGATATGCGGATATTCAATCGACAGTACGTGCGGTTAACGCAGGAGGGATTCATAC
CTACATCAGCAAACCTTGGGACAACGAAAACCTCAAGCTCATTGTAGGAAAAGCCGCCGAGTTTTATCGCTTAAGTCGGG
ATAAAGAGCGCCTGACTATTGAATTGGAAGAACGCAATAAAGAACTGGAAGTTGCGAATCAGGCATTAGCTGAGAATAAC
CATAAACTTGCGCAATTTAATCAAGAATTGGAGGCGAAAGTACAAGAGCGTACCGTCGCCTTACAAGAGAGTAACAAGCG
CTTAGAATCCTTGCTTGCTTCACGCAATAAAACGTTTAAAGATATTTTGGCTATGGTGACCGCGATTATCCAGCATCGTA
CGGGGTTCCCTGCGGATCATGCAGAGCGGATCGCAAATCAGGCCAAATCGGTCGCTCTTAAACTGAAATTATCAGAGGCC
GTTGCCAGCCATGTTTATTTATGTGGGCTTATGCATCAAATCGGCCTGATTGCTGAAACCAGTAATGACTGGAAAGTGGT
CAAGGTGCATCAGGATTCGGAGATCCCCATTACCCCGAACGTGAATCCGATTTTGGGTGCAGAAATTGTGGGCCGAATTA
AGCGCTTTGAACCGCTGATGGAAATTATCCGTCATCAAGATGAGCTGTATGATGGTACAGGGAAGCCGGATCATTTACGT
GGTGAGCAAATTCCGATTGGTGCGCGGATCATAAAAGTCGTCAAAGATTATGACTTCTTAGTTGCGGGGGCGAATAACCC
AAGACGCATGCACACCAAAAGTGCGCAAGGCTATCTGCGTCAGCAGTCCGAAGTCTATTACGATCCTAAAGTCGTGGAAG
CCTTTATTACGATTGTGAGTGCAGTGACTCGAATTGAAGAGGGTATGGAACTGTGTGTCAGCCTGAGCGAAGTGCGTCCG
GGCATGGTTATCAAACGTGATATCTATCTACCCAACGGCAATTTAATGCTGACGGCTGGCAACGCAATGAGTGAGCCTTT
ATTACGTAAACTCAAAGAGTTAGAACAAGAGATGAATATGCCGATCCCTGTTTATATAGGATAA

Upstream 100 bases:

>100_bases
GACCTGGTGCAAGGTTATTTAGTTAGCCCGGCTATTTCACCACAACAAGTCCTGAACTATCTCCATAACAAACTATCTCC
ATAAAAAATAGGGGAGCGCT

Downstream 100 bases:

>100_bases
GGGCATAGGATAAAGAGATAAGAATTAGTCAGGTCGTTACGGTTTGTTGGGGGCTAACGGTAGGGGGGCTAACGGTAGAG
TAATTAACACTCGGGTGCCT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MQMDNMHDEKLNVLLLDDENDILKALNRVLRMDYNVVTFDNGADALEYLQENPIHIIISDMRMPEMDGADFLAKAREMQP
DTVRLLLTGYADIQSTVRAVNAGGIHTYISKPWDNENLKLIVGKAAEFYRLSRDKERLTIELEERNKELEVANQALAENN
HKLAQFNQELEAKVQERTVALQESNKRLESLLASRNKTFKDILAMVTAIIQHRTGFPADHAERIANQAKSVALKLKLSEA
VASHVYLCGLMHQIGLIAETSNDWKVVKVHQDSEIPITPNVNPILGAEIVGRIKRFEPLMEIIRHQDELYDGTGKPDHLR
GEQIPIGARIIKVVKDYDFLVAGANNPRRMHTKSAQGYLRQQSEVYYDPKVVEAFITIVSAVTRIEEGMELCVSLSEVRP
GMVIKRDIYLPNGNLMLTAGNAMSEPLLRKLKELEQEMNMPIPVYIG

Sequences:

>Translated_447_residues
MQMDNMHDEKLNVLLLDDENDILKALNRVLRMDYNVVTFDNGADALEYLQENPIHIIISDMRMPEMDGADFLAKAREMQP
DTVRLLLTGYADIQSTVRAVNAGGIHTYISKPWDNENLKLIVGKAAEFYRLSRDKERLTIELEERNKELEVANQALAENN
HKLAQFNQELEAKVQERTVALQESNKRLESLLASRNKTFKDILAMVTAIIQHRTGFPADHAERIANQAKSVALKLKLSEA
VASHVYLCGLMHQIGLIAETSNDWKVVKVHQDSEIPITPNVNPILGAEIVGRIKRFEPLMEIIRHQDELYDGTGKPDHLR
GEQIPIGARIIKVVKDYDFLVAGANNPRRMHTKSAQGYLRQQSEVYYDPKVVEAFITIVSAVTRIEEGMELCVSLSEVRP
GMVIKRDIYLPNGNLMLTAGNAMSEPLLRKLKELEQEMNMPIPVYIG
>Mature_447_residues
MQMDNMHDEKLNVLLLDDENDILKALNRVLRMDYNVVTFDNGADALEYLQENPIHIIISDMRMPEMDGADFLAKAREMQP
DTVRLLLTGYADIQSTVRAVNAGGIHTYISKPWDNENLKLIVGKAAEFYRLSRDKERLTIELEERNKELEVANQALAENN
HKLAQFNQELEAKVQERTVALQESNKRLESLLASRNKTFKDILAMVTAIIQHRTGFPADHAERIANQAKSVALKLKLSEA
VASHVYLCGLMHQIGLIAETSNDWKVVKVHQDSEIPITPNVNPILGAEIVGRIKRFEPLMEIIRHQDELYDGTGKPDHLR
GEQIPIGARIIKVVKDYDFLVAGANNPRRMHTKSAQGYLRQQSEVYYDPKVVEAFITIVSAVTRIEEGMELCVSLSEVRP
GMVIKRDIYLPNGNLMLTAGNAMSEPLLRKLKELEQEMNMPIPVYIG

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=31.404958677686, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788550, Length=123, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1790299, Length=119, Percent_Identity=31.0924369747899, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788905, Length=94, Percent_Identity=34.0425531914894, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50642; Mature: 50642

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMDNMHDEKLNVLLLDDENDILKALNRVLRMDYNVVTFDNGADALEYLQENPIHIIISD
CCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCEEEEEEC
MRMPEMDGADFLAKAREMQPDTVRLLLTGYADIQSTVRAVNAGGIHTYISKPWDNENLKL
CCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEE
IVGKAAEFYRLSRDKERLTIELEERNKELEVANQALAENNHKLAQFNQELEAKVQERTVA
EEECCHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LQESNKRLESLLASRNKTFKDILAMVTAIIQHRTGFPADHAERIANQAKSVALKLKLSEA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEHHHH
VASHVYLCGLMHQIGLIAETSNDWKVVKVHQDSEIPITPNVNPILGAEIVGRIKRFEPLM
HHHHHHHHHHHHHHCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EIIRHQDELYDGTGKPDHLRGEQIPIGARIIKVVKDYDFLVAGANNPRRMHTKSAQGYLR
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCHHCCHHHHHHHH
QQSEVYYDPKVVEAFITIVSAVTRIEEGMELCVSLSEVRPGMVIKRDIYLPNGNLMLTAG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEC
NAMSEPLLRKLKELEQEMNMPIPVYIG
CHHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MQMDNMHDEKLNVLLLDDENDILKALNRVLRMDYNVVTFDNGADALEYLQENPIHIIISD
CCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCEEEEEEC
MRMPEMDGADFLAKAREMQPDTVRLLLTGYADIQSTVRAVNAGGIHTYISKPWDNENLKL
CCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEE
IVGKAAEFYRLSRDKERLTIELEERNKELEVANQALAENNHKLAQFNQELEAKVQERTVA
EEECCHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LQESNKRLESLLASRNKTFKDILAMVTAIIQHRTGFPADHAERIANQAKSVALKLKLSEA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEHHHH
VASHVYLCGLMHQIGLIAETSNDWKVVKVHQDSEIPITPNVNPILGAEIVGRIKRFEPLM
HHHHHHHHHHHHHHCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EIIRHQDELYDGTGKPDHLRGEQIPIGARIIKVVKDYDFLVAGANNPRRMHTKSAQGYLR
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCHHCCHHHHHHHH
QQSEVYYDPKVVEAFITIVSAVTRIEEGMELCVSLSEVRPGMVIKRDIYLPNGNLMLTAG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEC
NAMSEPLLRKLKELEQEMNMPIPVYIG
CHHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]