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The map label for this gene is gpsA

Identifier: 147673990

GI number: 147673990

Start: 2376927

End: 2377961

Strand: Reverse

Name: gpsA

Synonym: VC0395_A2226

Alternate gene names: 147673990

Gene position: 2377961-2376927 (Counterclockwise)

Preceding gene: 147674386

Following gene: 147673248

Centisome position: 78.63

GC content: 51.79

Gene sequence:

>1035_bases
ATGAGCGAAACACAGAACCACAATAGCTACGGAAAACCTGTTGAAATGACCGTGATAGGTGCGGGCTCCTACGGTACCTC
ACTGGCGATCTCTTTGGCGCGCAATGGCGCGAATATCGTCCTGTGGGGACATGATGCAGAGCATATGGCGCGCCTTGACG
CTGACCGCGCTAACCATGAATTTTTGCCCGGAATTGCTTTTCCTGACACTCTGATTGTGGAAACTGACTTGCAAAAAGCG
GTGCAAGCGAGCCGTGATCTGCTGGTGGTAGTGCCTAGCCATGTGTTTGGTATCGTGTTGAAAAGTTTGCAGCCACACCT
GCGTGCGGATAGCCGCATTTGTTGGGCAACCAAGGGGCTAGAGCCTGAGACTGGACGGCTACTCCAAGATGTCGCCCATG
ATGTACTTGGTGATAGCTACCCCCTTGCGGTGCTTTCTGGTCCTACTTTTGCCAAAGAGCTGGCGATGGGGATGCCAACC
GCGATTTCAGTGGCTTCGCCGGATGCGCAATTTGTACGTGACCTGCAAGAGAAAATTCACTGCAGCAAAACTTTCCGAGT
GTACGCCAACAGTGACTTTATCGGTATGCAGCTTGGCGGCGCGGTAAAAAACGTGATTGCGATTGGTGCGGGGATGTCTG
ATGGTATTGGCTTTGGCGCGAATGCGCGAACCGCTCTTATCACTCGTGGCTTGGCTGAAATGAGCCGCTTAGGCGCCGCT
TTAGGCGCGCAGCCGGAAACTTTTATGGGTATGGCAGGATTAGGTGATTTAGTGCTGACTTGTACTGACAACCAATCGCG
TAACCGCCGTTTTGGTTTGGCACTAGGTCAAGGTAAAGATGTGGATACCGCGCAAACCGATATCGGCCAAGTGGTTGAAG
GGTATCGCAACACCAAAGAAGTGTGGATGTTAGCGAAACGCATGGGCGTTGAGATGCCGATAGTTGAACAAATTTACCAA
GTATTGTATCAAGGAAAAGATGCGCGACTCGCCGCTCAGGATTTGCTGGCACGAGATAAGAAGATGGAGCGTTAA

Upstream 100 bases:

>100_bases
ACTCTTTATCTAAGGCAGCCTTGGCGAAGAAGGTGCAGACTGCAATAGCCTATGTTTGGGTATAGAATAGAGCACAACTT
CGTTATCGATAGGCGGAACA

Downstream 100 bases:

>100_bases
TCCGCAGCGCAAGAGATGGAATTGAATCGATAGGGTATAGCTTGCCCTATCTTGATTAGGACTGAGTAAGTAATGAAACA
GTGCGCCCACACCAAGGTTT

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSETQNHNSYGKPVEMTVIGAGSYGTSLAISLARNGANIVLWGHDAEHMARLDADRANHEFLPGIAFPDTLIVETDLQKA
VQASRDLLVVVPSHVFGIVLKSLQPHLRADSRICWATKGLEPETGRLLQDVAHDVLGDSYPLAVLSGPTFAKELAMGMPT
AISVASPDAQFVRDLQEKIHCSKTFRVYANSDFIGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAA
LGAQPETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGKDVDTAQTDIGQVVEGYRNTKEVWMLAKRMGVEMPIVEQIYQ
VLYQGKDARLAAQDLLARDKKMER

Sequences:

>Translated_344_residues
MSETQNHNSYGKPVEMTVIGAGSYGTSLAISLARNGANIVLWGHDAEHMARLDADRANHEFLPGIAFPDTLIVETDLQKA
VQASRDLLVVVPSHVFGIVLKSLQPHLRADSRICWATKGLEPETGRLLQDVAHDVLGDSYPLAVLSGPTFAKELAMGMPT
AISVASPDAQFVRDLQEKIHCSKTFRVYANSDFIGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAA
LGAQPETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGKDVDTAQTDIGQVVEGYRNTKEVWMLAKRMGVEMPIVEQIYQ
VLYQGKDARLAAQDLLARDKKMER
>Mature_343_residues
SETQNHNSYGKPVEMTVIGAGSYGTSLAISLARNGANIVLWGHDAEHMARLDADRANHEFLPGIAFPDTLIVETDLQKAV
QASRDLLVVVPSHVFGIVLKSLQPHLRADSRICWATKGLEPETGRLLQDVAHDVLGDSYPLAVLSGPTFAKELAMGMPTA
ISVASPDAQFVRDLQEKIHCSKTFRVYANSDFIGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAAL
GAQPETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGKDVDTAQTDIGQVVEGYRNTKEVWMLAKRMGVEMPIVEQIYQV
LYQGKDARLAAQDLLARDKKMER

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI33695088, Length=337, Percent_Identity=26.4094955489614, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI24307999, Length=336, Percent_Identity=25, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1790037, Length=329, Percent_Identity=74.7720364741641, Blast_Score=502, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI32564399, Length=332, Percent_Identity=24.6987951807229, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193210136, Length=341, Percent_Identity=24.3401759530792, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32564403, Length=341, Percent_Identity=24.3401759530792, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17507425, Length=337, Percent_Identity=23.1454005934718, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193210134, Length=330, Percent_Identity=22.7272727272727, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324513, Length=347, Percent_Identity=26.8011527377522, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6320181, Length=344, Percent_Identity=28.7790697674419, Blast_Score=112, Evalue=8e-26,
Organism=Drosophila melanogaster, GI17136204, Length=346, Percent_Identity=26.0115606936416, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI17136202, Length=346, Percent_Identity=26.0115606936416, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI17136200, Length=346, Percent_Identity=26.0115606936416, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI22026922, Length=328, Percent_Identity=22.8658536585366, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45551945, Length=264, Percent_Identity=25.3787878787879, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281362270, Length=264, Percent_Identity=25.3787878787879, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648969, Length=183, Percent_Identity=29.5081967213115, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_VIBC3 (A5F502)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F502
- SMR:   A5F502
- STRING:   A5F502
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2226
- eggNOG:   COG0240
- HOGENOM:   HBG586392
- OMA:   NVAKGIE
- ProtClustDB:   PRK00094
- BioCyc:   VCHO345073:VC0395_A2226-MONOMER
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 37110; Mature: 36979

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 203-203 BINDING 118-118 BINDING 118-118 BINDING 151-151 BINDING 267-267 BINDING 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETQNHNSYGKPVEMTVIGAGSYGTSLAISLARNGANIVLWGHDAEHMARLDADRANHE
CCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHCCCCCCC
FLPGIAFPDTLIVETDLQKAVQASRDLLVVVPSHVFGIVLKSLQPHLRADSRICWATKGL
CCCCCCCCCCEEEEHHHHHHHHCCCCEEEEECHHHHHHHHHHHCHHHCCCCEEEEEECCC
EPETGRLLQDVAHDVLGDSYPLAVLSGPTFAKELAMGMPTAISVASPDAQFVRDLQEKIH
CCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
CSKTFRVYANSDFIGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAA
HCCEEEEEECCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGAQPETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGKDVDTAQTDIGQVVEGYRNTKE
HCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHH
VWMLAKRMGVEMPIVEQIYQVLYQGKDARLAAQDLLARDKKMER
HHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SETQNHNSYGKPVEMTVIGAGSYGTSLAISLARNGANIVLWGHDAEHMARLDADRANHE
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHCCCCCCC
FLPGIAFPDTLIVETDLQKAVQASRDLLVVVPSHVFGIVLKSLQPHLRADSRICWATKGL
CCCCCCCCCCEEEEHHHHHHHHCCCCEEEEECHHHHHHHHHHHCHHHCCCCEEEEEECCC
EPETGRLLQDVAHDVLGDSYPLAVLSGPTFAKELAMGMPTAISVASPDAQFVRDLQEKIH
CCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
CSKTFRVYANSDFIGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAA
HCCEEEEEECCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGAQPETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGKDVDTAQTDIGQVVEGYRNTKE
HCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHH
VWMLAKRMGVEMPIVEQIYQVLYQGKDARLAAQDLLARDKKMER
HHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA