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The map label for this gene is purA

Identifier: 147673842

GI number: 147673842

Start: 2325230

End: 2326528

Strand: Reverse

Name: purA

Synonym: VC0395_A2180

Alternate gene names: 147673842

Gene position: 2326528-2325230 (Counterclockwise)

Preceding gene: 147674447

Following gene: 147674337

Centisome position: 76.93

GC content: 49.11

Gene sequence:

>1299_bases
ATGGGTAATAACGTTGTCGTTCTTGGCACCCAATGGGGTGACGAAGGTAAAGGTAAAATCGTAGACCTTTTAACTGAAGA
TGCAAAATACGTGGTTCGCTACCAAGGCGGACACAACGCAGGTCACACACTAGTAATCGACGGTCAAAAAACCGTTCTCC
ACTTAATTCCATCCGGTATTCTTCGCAATAACGTGAAATGCATCATCGGTAACGGTGTAGTACTTTCACCAGAAGCATTG
ATCAAAGAAATGTCTGGTCTCGAAGAGCGTGGTGTTCCTGTTCGCGAGCGTCTTTTCATTTCTGAAGCTTGTCCTCTGAT
CCTGCCATACCACGTTGCTCTTGACCAAGCGCGCGAAGCCGCTCGTGGCAAGAAAGCAATCGGTACTACTGGTCGTGGTA
TTGGTCCTGCGTACGAAGATAAAGTAGCTCGTCGTGGTCTGCGTGTTGGCGACCTATTCGATATGGCTTCTTTTGCTGAG
AAACTGCAAGAAGTGATGGCATTCCATAACTTCCAACTTGAGCACTTCTACAAAGTAGAACCAGTAAGCTACGAAGCGGT
ACTGGAGCAGGCGAAGGGTTATGCAGAACTGCTGACTTCTATGGTTATCGACGTAACCAACGAACTGGATGCAGCGCGTA
AGCGTGGCGACAAAATCATGTTCGAAGGCGCGCAAGGTACACTACTGGATATCGACCACGGTACTTATCCATATGTAACG
TCTTCAAACACCACTGCTGGTGGTGTTGCTGCGGGATCTGGTTTTGGTCCTCGTCACCTAGGTTACATCCTTGGTATCGC
GAAAGCGTACTGCACTCGTGTTGGTGCCGGTCCGTTCCCAACTGAACTGTTTGACGAAGTGGGCGATCATTTAGGCACTA
AAGGCCATGAGTTTGGTGCAACCACAGGTCGTAAGCGTCGTTGTGGTTGGTTTGATGCAGTGGCAATGCGCCGCGCGATC
CAAATCAACTCAGTCACCGGCTTCTGCCTGACCAAACTGGACGTTCTAGATGGTCTGAAAGAGATCAAAATCTGTACGGG
TTACCAAATGCCTGACGGTTCTATTGCTGAAGTTTCACCTATGGCCGCTGACGCATTTGAAAACGTGACGCCAATTTTCG
AAACTATGCCTGGTTGGTCTGAAACGACTTTCGGTGCAAAAACGCTGGCTGAACTGCCACAGACAGCACTGGATTACATC
AAGCGCATAGAAGAGCTGACTGGCGTTCCGGTAGACATCATTTCTACTGGCCCAGACCGCAACGAAACCATCATCAAAGT
TCACCCATTCAGCGCGTGA

Upstream 100 bases:

>100_bases
CAAAGCCGTTTTATATGAATTTTTCATGCTTAAAAAATGACTGCAAGGATTCAAATTCGGTGCTAGAATCCTTTTTTAAC
TAGCAATCAGACTTGGAAAG

Downstream 100 bases:

>100_bases
TCTTGAGATAAAAAACAGTAAGCCGACTCACCGAGTCGGCTTTTTTTTGCCATACTCTTGGTGGTATTTTGTGCGCCTGT
GGCAAAAAGTTGCCGCAAAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGQKTVLHLIPSGILRNNVKCIIGNGVVLSPEAL
IKEMSGLEERGVPVRERLFISEACPLILPYHVALDQAREAARGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFDMASFAE
KLQEVMAFHNFQLEHFYKVEPVSYEAVLEQAKGYAELLTSMVIDVTNELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVAAGSGFGPRHLGYILGIAKAYCTRVGAGPFPTELFDEVGDHLGTKGHEFGATTGRKRRCGWFDAVAMRRAI
QINSVTGFCLTKLDVLDGLKEIKICTGYQMPDGSIAEVSPMAADAFENVTPIFETMPGWSETTFGAKTLAELPQTALDYI
KRIEELTGVPVDIISTGPDRNETIIKVHPFSA

Sequences:

>Translated_432_residues
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGQKTVLHLIPSGILRNNVKCIIGNGVVLSPEAL
IKEMSGLEERGVPVRERLFISEACPLILPYHVALDQAREAARGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFDMASFAE
KLQEVMAFHNFQLEHFYKVEPVSYEAVLEQAKGYAELLTSMVIDVTNELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVAAGSGFGPRHLGYILGIAKAYCTRVGAGPFPTELFDEVGDHLGTKGHEFGATTGRKRRCGWFDAVAMRRAI
QINSVTGFCLTKLDVLDGLKEIKICTGYQMPDGSIAEVSPMAADAFENVTPIFETMPGWSETTFGAKTLAELPQTALDYI
KRIEELTGVPVDIISTGPDRNETIIKVHPFSA
>Mature_431_residues
GNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGQKTVLHLIPSGILRNNVKCIIGNGVVLSPEALI
KEMSGLEERGVPVRERLFISEACPLILPYHVALDQAREAARGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFDMASFAEK
LQEVMAFHNFQLEHFYKVEPVSYEAVLEQAKGYAELLTSMVIDVTNELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVTS
SNTTAGGVAAGSGFGPRHLGYILGIAKAYCTRVGAGPFPTELFDEVGDHLGTKGHEFGATTGRKRRCGWFDAVAMRRAIQ
INSVTGFCLTKLDVLDGLKEIKICTGYQMPDGSIAEVSPMAADAFENVTPIFETMPGWSETTFGAKTLAELPQTALDYIK
RIEELTGVPVDIISTGPDRNETIIKVHPFSA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=44, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=44.6759259259259, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=41.9191919191919, Blast_Score=297, Evalue=2e-80,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=75.6944444444444, Blast_Score=689, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=433, Percent_Identity=42.9561200923788, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=42.9561200923788, Blast_Score=332, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=43.7209302325581, Blast_Score=331, Evalue=2e-91,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=43.2941176470588, Blast_Score=349, Evalue=2e-96,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_VIBC3 (A5F534)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F534
- SMR:   A5F534
- STRING:   A5F534
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2180
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK01117
- BioCyc:   VCHO345073:VC0395_A2180-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46833; Mature: 46702

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGQKTVLHLIPSGI
CCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCHHEEHHHCHHH
LRNNVKCIIGNGVVLSPEALIKEMSGLEERGVPVRERLFISEACPLILPYHVALDQAREA
HHCCCEEEEECCEEECHHHHHHHHHCHHHCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHH
ARGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFDMASFAEKLQEVMAFHNFQLEHFYKVE
HCCCHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEC
PVSYEAVLEQAKGYAELLTSMVIDVTNELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVAAGSGFGPRHLGYILGIAKAYCTRVGAGPFPTELFDEVGDHLGTKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
TTGRKRRCGWFDAVAMRRAIQINSVTGFCLTKLDVLDGLKEIKICTGYQMPDGSIAEVSP
CCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHCCHHHHEEEECCCCCCCCCCCCCC
MAADAFENVTPIFETMPGWSETTFGAKTLAELPQTALDYIKRIEELTGVPVDIISTGPDR
HHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCC
NETIIKVHPFSA
CCEEEEEECCCC
>Mature Secondary Structure 
GNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGQKTVLHLIPSGI
CCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCHHEEHHHCHHH
LRNNVKCIIGNGVVLSPEALIKEMSGLEERGVPVRERLFISEACPLILPYHVALDQAREA
HHCCCEEEEECCEEECHHHHHHHHHCHHHCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHH
ARGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFDMASFAEKLQEVMAFHNFQLEHFYKVE
HCCCHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEC
PVSYEAVLEQAKGYAELLTSMVIDVTNELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVAAGSGFGPRHLGYILGIAKAYCTRVGAGPFPTELFDEVGDHLGTKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
TTGRKRRCGWFDAVAMRRAIQINSVTGFCLTKLDVLDGLKEIKICTGYQMPDGSIAEVSP
CCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHCCHHHHEEEECCCCCCCCCCCCCC
MAADAFENVTPIFETMPGWSETTFGAKTLAELPQTALDYIKRIEELTGVPVDIISTGPDR
HHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCC
NETIIKVHPFSA
CCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA