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The map label for this gene is mutH

Identifier: 147673814

GI number: 147673814

Start: 206625

End: 207290

Strand: Reverse

Name: mutH

Synonym: VC0395_A0200

Alternate gene names: 147673814

Gene position: 207290-206625 (Counterclockwise)

Preceding gene: 147674981

Following gene: 147673112

Centisome position: 6.85

GC content: 52.1

Gene sequence:

>666_bases
ATGAAACCAGCACCGACGACTCAACAAGAATTACTCACGCGAGCCCAACAAATTGCAGGGCTGAGTTTTGCTGAACTCGC
CGATGAAGCAGGCATGACCGTACCGCCAGATCTGCGTAAAGACAAAGGCTGGGTAGGCCAGTTACTGGAATGGCACTTAG
GGGCGACAGCAGGCAGTCGTCCACAACAAGATTTTGAACATCTTGGGATTGAGTTAAAGAGCATTCCCATCAGTTACACC
GGAAAACCGTTGGAGACCACTTTCGTTTGCGTCGCCCCACTCACCGGAGTTCATGGCTTAACATGGGAGCAAAGTCATGT
GCGCAATAAGCTCTCTAAGGTGCTCTGGATCCCGGTACAAGGTGAGCGCGAGATCCCGCTCGCTGAGCGCTGTGTGGGCT
ATCCTTTGCTATGGAGCCCTTCTCCCGAAGAGGAAGCGCAGCTCAAAGCGGACTGGGAAGAGTTAATGGAATTGATTGTG
CTAGGTAAAGTAGCACAAATTACCGCTAAACACGGTGAAGTGCTGCAACTGCGCCCTAAAGCCGCCAATGGTCGAGCGCT
CACTGAAGCCTATGGAGCCAATGGACGCCCAATCAAAACCCTGCCACGTGGTTTTTATCTGCGTACCCAATTTACTGCGC
AAATTCTGCAGCGCTATTACGCCTAG

Upstream 100 bases:

>100_bases
CCTCTTAATCTCATTTCAAGTATCAAAAATCGTTCATCCATGGCAAGCGCAAGCAAAATCAGTACAATCCCTACTGTGAT
TATCCAAACCGAAAGCCTTT

Downstream 100 bases:

>100_bases
TTATCTTCACAACGATCTTTTTTGTTTTCGCAGTGTCGTGACTCGCTTATAGTGAAAAAACTGTCTCCCTCGTGACTGGG
TTCTATTTGTGGCGACAGCG

Product: DNA mismatch repair protein

Products: NA

Alternate protein names: Methyl-directed mismatch repair protein

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKPAPTTQQELLTRAQQIAGLSFAELADEAGMTVPPDLRKDKGWVGQLLEWHLGATAGSRPQQDFEHLGIELKSIPISYT
GKPLETTFVCVAPLTGVHGLTWEQSHVRNKLSKVLWIPVQGEREIPLAERCVGYPLLWSPSPEEEAQLKADWEELMELIV
LGKVAQITAKHGEVLQLRPKAANGRALTEAYGANGRPIKTLPRGFYLRTQFTAQILQRYYA

Sequences:

>Translated_221_residues
MKPAPTTQQELLTRAQQIAGLSFAELADEAGMTVPPDLRKDKGWVGQLLEWHLGATAGSRPQQDFEHLGIELKSIPISYT
GKPLETTFVCVAPLTGVHGLTWEQSHVRNKLSKVLWIPVQGEREIPLAERCVGYPLLWSPSPEEEAQLKADWEELMELIV
LGKVAQITAKHGEVLQLRPKAANGRALTEAYGANGRPIKTLPRGFYLRTQFTAQILQRYYA
>Mature_221_residues
MKPAPTTQQELLTRAQQIAGLSFAELADEAGMTVPPDLRKDKGWVGQLLEWHLGATAGSRPQQDFEHLGIELKSIPISYT
GKPLETTFVCVAPLTGVHGLTWEQSHVRNKLSKVLWIPVQGEREIPLAERCVGYPLLWSPSPEEEAQLKADWEELMELIV
LGKVAQITAKHGEVLQLRPKAANGRALTEAYGANGRPIKTLPRGFYLRTQFTAQILQRYYA

Specific function: Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair

COG id: COG3066

COG function: function code L; DNA mismatch repair protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mutH family

Homologues:

Organism=Escherichia coli, GI1789196, Length=216, Percent_Identity=62.962962962963, Blast_Score=290, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MUTH_VIBC3 (A5F8Z6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F8Z6
- SMR:   A5F8Z6
- STRING:   A5F8Z6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0200
- eggNOG:   COG3066
- HOGENOM:   HBG296591
- OMA:   QDWEELM
- ProtClustDB:   PRK05070
- BioCyc:   VCHO345073:VC0395_A0200-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00759
- InterPro:   IPR004230
- InterPro:   IPR011337
- InterPro:   IPR011335
- Gene3D:   G3DSA:3.40.600.10
- SMART:   SM00927
- TIGRFAMs:   TIGR02248

Pfam domain/function: PF02976 MutH; SSF52980 Restrict_endonuc_II-like_core

EC number: NA

Molecular weight: Translated: 24618; Mature: 24618

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPAPTTQQELLTRAQQIAGLSFAELADEAGMTVPPDLRKDKGWVGQLLEWHLGATAGSR
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PQQDFEHLGIELKSIPISYTGKPLETTFVCVAPLTGVHGLTWEQSHVRNKLSKVLWIPVQ
CHHHHHHHCEEEEECCEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHEEEEECC
GEREIPLAERCVGYPLLWSPSPEEEAQLKADWEELMELIVLGKVAQITAKHGEVLQLRPK
CCCCCCHHHHHCCCCEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
AANGRALTEAYGANGRPIKTLPRGFYLRTQFTAQILQRYYA
CCCCCCHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPAPTTQQELLTRAQQIAGLSFAELADEAGMTVPPDLRKDKGWVGQLLEWHLGATAGSR
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PQQDFEHLGIELKSIPISYTGKPLETTFVCVAPLTGVHGLTWEQSHVRNKLSKVLWIPVQ
CHHHHHHHCEEEEECCEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHEEEEECC
GEREIPLAERCVGYPLLWSPSPEEEAQLKADWEELMELIVLGKVAQITAKHGEVLQLRPK
CCCCCCHHHHHCCCCEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
AANGRALTEAYGANGRPIKTLPRGFYLRTQFTAQILQRYYA
CCCCCCHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA