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The map label for this gene is citS [H]

Identifier: 147673767

GI number: 147673767

Start: 1271722

End: 1273338

Strand: Reverse

Name: citS [H]

Synonym: VC0395_A1210

Alternate gene names: 147673767

Gene position: 1273338-1271722 (Counterclockwise)

Preceding gene: 147674760

Following gene: 147673209

Centisome position: 42.11

GC content: 48.55

Gene sequence:

>1617_bases
ATGGTGTGGAGCAGTATCACCTTCCGCAAGAGAATGCTGATCATCATGACCTTATCGGGTCTGATTGAGCTGCTACTTTT
GGTTGCCGCAGGGTTTATGTACGTCAAACATTCTCAAGAGCAAGAGGTTGGCCAGAAAGCGCTGGGCGTTGCGGCATTCC
TTGCACAAACTCCAGCGGTCATCAACATGATCAAGACCGGCCGTGCTTCCGATGATCAGCAGCAAAGCTATCGTGAACTG
ACTCAACTAATAGGCGCAGCGTTTATTGTGATTGGCGACAATCAAGGTATTCGATTAGTTCATCCCATTGATGAAAGGAT
CGGTAAGCCTATGGTGGGGGGCGATAACGAGAGAGCGCTAGTGGAAGGCGAAGCTTATGTCTCTTTCGCTCAAGGTTCGT
TAGGCAAATCGGTTCGTGGTAAAGCGGCGGTGGTGGATCAACACGGTCAAATCATCGGTGTCGTTTCGGTTGGCTATTTA
ATTGAGCGGCTACAAGACCGCGTTGAGCCATTTTTATTCTATCTGATTGCTACCGCGTTACTGGTGGTAGGCGCCAATGC
ACTGGTTTCTAGCTATGCCTCACGACGTTTTCAGCGCGCTATTTTGGGGTTTGAGCCGGAAGAAATTGCCCGTTTGTACG
TGGAACTGGATGTGACCATGAGCACCCTCAAAGAAGGGATCATCAGTATTGATGACAAAGGGATCTTACGTTCGATCAAT
AAAAGTGCTTGTGAAATTTTGGGATTACGCCGCGAACAAGCTCTTAATCGACGCTTGACCACCATTCTGCCAGAAAGTGA
TCTCTATACGGTTTTGGCGACCGGAAGAACCGATCACGATATTGAGCTCTATCTGAATAAGCATTGGATAATTGCCAACC
GCAGCCCGATCGTGGTTGAGGGAAAAGTCGTCGGGGCGGTATCCAGTTTCCGTCTCCGTGATGAAATTAATGAACTCACT
GAGCAGTTGGCCCAAACCCGTGAGTACGCAGAGATGCTGCGTTCTCAGACCCATGAGCACAGGAATAAATTGAATACTAT
TAGTGGGTTACTGCAAATGGGTGAATTGGATGCCGTGCAGCAACTGATTGGTCAAGAAACTGAGCACTACCAAGTGTTGA
TTGAGTTTTTACGTGAAACAATCAAAGACCCTCTGATTGCAGGGATGCTGCTTGGCAAAACAGAGCGCGCTCGCGAGCTG
GGCTTGGAACTCATGGTGGAAGACGGGGCACGTTTAGAAACGCTGCCGATTCATATTAAAGCGGAAGATGTGGTGACCAT
TCTCGGTAACCTGATCGATAACGCGTTTGAAGCGACCTTAACCGCGATACGTACGCTGTCGCCAGTTCCACCGGAACGCA
GAGTGATTGAGGTCTCGATCAGTGATTTTGGTAACGAAATCATTTTGGAAGTGGATGATCAAGGTTGCGGTTTACCTAAA
GAGCTGGAACATTGGCAGCTAACGGAAAAAGGGGTGTCGAGCAAAGCCGTGCAAAACCGAGGGGTGGGGCTGTTTTTAGT
CAAACAGCTTGCCGATCGTTATCAAGGACAACTGGACATGCACAGCAAAGCGCAAGGTACTCGAATGACAGTGTATTTAC
CCAAGGATGAGTTGTAA

Upstream 100 bases:

>100_bases
GGCTAACGTGAATCTCGTGCCAGCCTCACAGTTATGTATTGCGAGCTTCGCCCCAATGTTAAATTGATGTTAGCTTTCAC
TCGCTAGCAAGGAGTGAACA

Downstream 100 bases:

>100_bases
TGAAAACCCGACTCCTGATCATTGAAGATGATGCTGCGATAGCGCAGCTTCATCATCGCTATTTAAGTCAGTTAGAAGGC
TTTGAGGTAGTGGGGATCGC

Product: putative sensor kinase citA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MVWSSITFRKRMLIIMTLSGLIELLLLVAAGFMYVKHSQEQEVGQKALGVAAFLAQTPAVINMIKTGRASDDQQQSYREL
TQLIGAAFIVIGDNQGIRLVHPIDERIGKPMVGGDNERALVEGEAYVSFAQGSLGKSVRGKAAVVDQHGQIIGVVSVGYL
IERLQDRVEPFLFYLIATALLVVGANALVSSYASRRFQRAILGFEPEEIARLYVELDVTMSTLKEGIISIDDKGILRSIN
KSACEILGLRREQALNRRLTTILPESDLYTVLATGRTDHDIELYLNKHWIIANRSPIVVEGKVVGAVSSFRLRDEINELT
EQLAQTREYAEMLRSQTHEHRNKLNTISGLLQMGELDAVQQLIGQETEHYQVLIEFLRETIKDPLIAGMLLGKTERAREL
GLELMVEDGARLETLPIHIKAEDVVTILGNLIDNAFEATLTAIRTLSPVPPERRVIEVSISDFGNEIILEVDDQGCGLPK
ELEHWQLTEKGVSSKAVQNRGVGLFLVKQLADRYQGQLDMHSKAQGTRMTVYLPKDEL

Sequences:

>Translated_538_residues
MVWSSITFRKRMLIIMTLSGLIELLLLVAAGFMYVKHSQEQEVGQKALGVAAFLAQTPAVINMIKTGRASDDQQQSYREL
TQLIGAAFIVIGDNQGIRLVHPIDERIGKPMVGGDNERALVEGEAYVSFAQGSLGKSVRGKAAVVDQHGQIIGVVSVGYL
IERLQDRVEPFLFYLIATALLVVGANALVSSYASRRFQRAILGFEPEEIARLYVELDVTMSTLKEGIISIDDKGILRSIN
KSACEILGLRREQALNRRLTTILPESDLYTVLATGRTDHDIELYLNKHWIIANRSPIVVEGKVVGAVSSFRLRDEINELT
EQLAQTREYAEMLRSQTHEHRNKLNTISGLLQMGELDAVQQLIGQETEHYQVLIEFLRETIKDPLIAGMLLGKTERAREL
GLELMVEDGARLETLPIHIKAEDVVTILGNLIDNAFEATLTAIRTLSPVPPERRVIEVSISDFGNEIILEVDDQGCGLPK
ELEHWQLTEKGVSSKAVQNRGVGLFLVKQLADRYQGQLDMHSKAQGTRMTVYLPKDEL
>Mature_538_residues
MVWSSITFRKRMLIIMTLSGLIELLLLVAAGFMYVKHSQEQEVGQKALGVAAFLAQTPAVINMIKTGRASDDQQQSYREL
TQLIGAAFIVIGDNQGIRLVHPIDERIGKPMVGGDNERALVEGEAYVSFAQGSLGKSVRGKAAVVDQHGQIIGVVSVGYL
IERLQDRVEPFLFYLIATALLVVGANALVSSYASRRFQRAILGFEPEEIARLYVELDVTMSTLKEGIISIDDKGILRSIN
KSACEILGLRREQALNRRLTTILPESDLYTVLATGRTDHDIELYLNKHWIIANRSPIVVEGKVVGAVSSFRLRDEINELT
EQLAQTREYAEMLRSQTHEHRNKLNTISGLLQMGELDAVQQLIGQETEHYQVLIEFLRETIKDPLIAGMLLGKTERAREL
GLELMVEDGARLETLPIHIKAEDVVTILGNLIDNAFEATLTAIRTLSPVPPERRVIEVSISDFGNEIILEVDDQGCGLPK
ELEHWQLTEKGVSSKAVQNRGVGLFLVKQLADRYQGQLDMHSKAQGTRMTVYLPKDEL

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=549, Percent_Identity=32.7868852459016, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1790567, Length=536, Percent_Identity=30.410447761194, Blast_Score=212, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59886; Mature: 59886

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00014 ER_TARGET ; PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWSSITFRKRMLIIMTLSGLIELLLLVAAGFMYVKHSQEQEVGQKALGVAAFLAQTPAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHH
INMIKTGRASDDQQQSYRELTQLIGAAFIVIGDNQGIRLVHPIDERIGKPMVGGDNERAL
HHHHHCCCCCCHHHHHHHHHHHHHCEEEEEEECCCCEEEECCHHHHHCCCCCCCCCCCEE
VEGEAYVSFAQGSLGKSVRGKAAVVDQHGQIIGVVSVGYLIERLQDRVEPFLFYLIATAL
EECCHHEEECCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVVGANALVSSYASRRFQRAILGFEPEEIARLYVELDVTMSTLKEGIISIDDKGILRSIN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHH
KSACEILGLRREQALNRRLTTILPESDLYTVLATGRTDHDIELYLNKHWIIANRSPIVVE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECEEEEECCCCEEEE
GKVVGAVSSFRLRDEINELTEQLAQTREYAEMLRSQTHEHRNKLNTISGLLQMGELDAVQ
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
QLIGQETEHYQVLIEFLRETIKDPLIAGMLLGKTERARELGLELMVEDGARLETLPIHIK
HHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHCCCEEEECCCCCEEEEEEEEE
AEDVVTILGNLIDNAFEATLTAIRTLSPVPPERRVIEVSISDFGNEIILEVDDQGCGLPK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCH
ELEHWQLTEKGVSSKAVQNRGVGLFLVKQLADRYQGQLDMHSKAQGTRMTVYLPKDEL
HHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MVWSSITFRKRMLIIMTLSGLIELLLLVAAGFMYVKHSQEQEVGQKALGVAAFLAQTPAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHH
INMIKTGRASDDQQQSYRELTQLIGAAFIVIGDNQGIRLVHPIDERIGKPMVGGDNERAL
HHHHHCCCCCCHHHHHHHHHHHHHCEEEEEEECCCCEEEECCHHHHHCCCCCCCCCCCEE
VEGEAYVSFAQGSLGKSVRGKAAVVDQHGQIIGVVSVGYLIERLQDRVEPFLFYLIATAL
EECCHHEEECCCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVVGANALVSSYASRRFQRAILGFEPEEIARLYVELDVTMSTLKEGIISIDDKGILRSIN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHH
KSACEILGLRREQALNRRLTTILPESDLYTVLATGRTDHDIELYLNKHWIIANRSPIVVE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECEEEEECCCCEEEE
GKVVGAVSSFRLRDEINELTEQLAQTREYAEMLRSQTHEHRNKLNTISGLLQMGELDAVQ
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
QLIGQETEHYQVLIEFLRETIKDPLIAGMLLGKTERARELGLELMVEDGARLETLPIHIK
HHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHCCCEEEECCCCCEEEEEEEEE
AEDVVTILGNLIDNAFEATLTAIRTLSPVPPERRVIEVSISDFGNEIILEVDDQGCGLPK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCH
ELEHWQLTEKGVSSKAVQNRGVGLFLVKQLADRYQGQLDMHSKAQGTRMTVYLPKDEL
HHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]