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The map label for this gene is citT [H]

Identifier: 147673209

GI number: 147673209

Start: 1271036

End: 1271722

Strand: Reverse

Name: citT [H]

Synonym: VC0395_A1209

Alternate gene names: 147673209

Gene position: 1271722-1271036 (Counterclockwise)

Preceding gene: 147673767

Following gene: 147675751

Centisome position: 42.05

GC content: 49.2

Gene sequence:

>687_bases
ATGAAAACCCGACTCCTGATCATTGAAGATGATGCTGCGATAGCGCAGCTTCATCATCGCTATTTAAGTCAGTTAGAAGG
CTTTGAGGTAGTGGGGATCGCGATGTCTCAAGCGGATGCGCGTTTACAGATGGAAGTGCTTAACCCTGATTTGGTGCTGT
TGGACGTTTATTTACCGGATGGAACGGGCCTTGAGCTGTTGCAATGGATTCGTGGACGTAATGTTCATTGCGATGTGATT
TTAATTACGGCGGCGCGCGAGGTGGAGACACTGCAACAAGCGATGCGTGGTGGTGTGGTGGATTATCTGCTCAAGCCTGT
GATGTTTCCAAGGCTGGAAACCGCACTAAGAAAATACCAAATGCGGCAAGCTGAGCTAAAACAGTTGGCGGATTTTGATC
AGCAGAAAGTCGACAAAATGTTCACCACACATTCCTCAGAAGCCTTACCGCAACGTTTACCGAAAGGCATTGACGCCGTG
ACATTGGATAAGATTCGGTTGCTATTTCAAACGGCTACCATGCTGACGGCGGACGAGGCAGGTGAAAAAATTGGGGCTAG
CCGCACAACAGCCCGACGCTATCTTGAGTATCTTATCACTGCTGGAGAGTTGGAGGCCGATCTCAGTTATGGTACGGTGG
GACGCCCAGAACGAACGTACCGTAAATTACATCGTCACACATCCTGA

Upstream 100 bases:

>100_bases
TAGTCAAACAGCTTGCCGATCGTTATCAAGGACAACTGGACATGCACAGCAAAGCGCAAGGTACTCGAATGACAGTGTAT
TTACCCAAGGATGAGTTGTA

Downstream 100 bases:

>100_bases
TTAGACGGATTGGATAACTGCATCTAAAGTTTAAACAGATTACACGAATGTGATGAGGTCGACAGATGCAATGGCGTTTT
TGTTTGACAACAGTACTGGG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKTRLLIIEDDAAIAQLHHRYLSQLEGFEVVGIAMSQADARLQMEVLNPDLVLLDVYLPDGTGLELLQWIRGRNVHCDVI
LITAAREVETLQQAMRGGVVDYLLKPVMFPRLETALRKYQMRQAELKQLADFDQQKVDKMFTTHSSEALPQRLPKGIDAV
TLDKIRLLFQTATMLTADEAGEKIGASRTTARRYLEYLITAGELEADLSYGTVGRPERTYRKLHRHTS

Sequences:

>Translated_228_residues
MKTRLLIIEDDAAIAQLHHRYLSQLEGFEVVGIAMSQADARLQMEVLNPDLVLLDVYLPDGTGLELLQWIRGRNVHCDVI
LITAAREVETLQQAMRGGVVDYLLKPVMFPRLETALRKYQMRQAELKQLADFDQQKVDKMFTTHSSEALPQRLPKGIDAV
TLDKIRLLFQTATMLTADEAGEKIGASRTTARRYLEYLITAGELEADLSYGTVGRPERTYRKLHRHTS
>Mature_228_residues
MKTRLLIIEDDAAIAQLHHRYLSQLEGFEVVGIAMSQADARLQMEVLNPDLVLLDVYLPDGTGLELLQWIRGRNVHCDVI
LITAAREVETLQQAMRGGVVDYLLKPVMFPRLETALRKYQMRQAELKQLADFDQQKVDKMFTTHSSEALPQRLPKGIDAV
TLDKIRLLFQTATMLTADEAGEKIGASRTTARRYLEYLITAGELEADLSYGTVGRPERTYRKLHRHTS

Specific function: Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Phosphorylated CitT binds to the citM promoter to activate the transcription of the citM-yflN operon [H]

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786838, Length=217, Percent_Identity=38.7096774193548, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1790566, Length=233, Percent_Identity=34.3347639484979, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788550, Length=179, Percent_Identity=25.6983240223464, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR013196
- InterPro:   IPR001789 [H]

Pfam domain/function: PF08279 HTH_11; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25894; Mature: 25894

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRLLIIEDDAAIAQLHHRYLSQLEGFEVVGIAMSQADARLQMEVLNPDLVLLDVYLPD
CCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEHHHCCHHEEEEECCCCEEEEEEECCC
GTGLELLQWIRGRNVHCDVILITAAREVETLQQAMRGGVVDYLLKPVMFPRLETALRKYQ
CCCHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MRQAELKQLADFDQQKVDKMFTTHSSEALPQRLPKGIDAVTLDKIRLLFQTATMLTADEA
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GEKIGASRTTARRYLEYLITAGELEADLSYGTVGRPERTYRKLHRHTS
HHHHCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKTRLLIIEDDAAIAQLHHRYLSQLEGFEVVGIAMSQADARLQMEVLNPDLVLLDVYLPD
CCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEHHHCCHHEEEEECCCCEEEEEEECCC
GTGLELLQWIRGRNVHCDVILITAAREVETLQQAMRGGVVDYLLKPVMFPRLETALRKYQ
CCCHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MRQAELKQLADFDQQKVDKMFTTHSSEALPQRLPKGIDAVTLDKIRLLFQTATMLTADEA
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GEKIGASRTTARRYLEYLITAGELEADLSYGTVGRPERTYRKLHRHTS
HHHHCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 10972810 [H]