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The map label for this gene is epsL [H]

Identifier: 147673681

GI number: 147673681

Start: 2452040

End: 2453251

Strand: Reverse

Name: epsL [H]

Synonym: VC0395_A2298

Alternate gene names: 147673681

Gene position: 2453251-2452040 (Counterclockwise)

Preceding gene: 147675385

Following gene: 147674283

Centisome position: 81.12

GC content: 51.16

Gene sequence:

>1212_bases
GTGAGCGAGTTTCTGACCGTTCGACTGAGTAGTCAAAAAGAGGCCGATATCCCTTGGCTGGTTTGGTCTGCCGAGCAGCA
AGAAGTGATTGCCAGCGGCCAAGTGGCTGGTTGGGAAGCCTTGCATGAAATTGAGTCTTATGCTGATCAGCGCAGCGTAG
TGGTATTACTGGCGGCGAGTGATTTGATTTTAACGTCAGTGGAGATCCCGCCCGGCGCTTCTCGTCAGCTTGAAAATATG
CTGCCGTATTTGTTAGAAGATGAAATCGCCCAAGATGTGGAAGATGTGCACTTTTGTGTGCTGAGTAAAGGACGAGAAAC
GGCGGATGTGGTCGGTGTCGATCGTCTTTGGCTGCGCGCTTGCTTAGATCATCTCAAAGCGTGCGGTTTTGATGTGAAGC
GCGTATTGCCTGATGTACTGGCGATCCCTCGCCCAGAGCACGGTTTAGCTGCCCTGCAATTGGGTGATGAGTGGTTAGTG
CGTAAAAGCACTACGCAAGGGATGGCAGTGGACGCGCAGTGGTTAAGCTTACTGGCCGCTTCCGATTGGGTGCAGAATGA
GGGTGAGTATTTGCCTTTGCAAGCGCTCACGCCGCTTCCAGAGCTGAGCTTAGCCGAAACCCAAGAGTGGCGTTATGAGC
CGAGCGGTTTAGTCATGCAACTGCTGACTCAGGAGGCCTTAACCAGCAAGTTCAACTTGCTGACGGGGAGTTTTAAACTC
AAGTCTTCTTGGCTGCGGTATTGGCAAATATGGCGCAAAGTGGCGATCGCTGCTGGGCTATTTGTCGCGGTATCCATCAG
TTATTCGCTGTTTCAGGCGCATCAATACGAAGCACAAGCGGACGCTTACCGCGCGGAAAGTGAGCGGATTTTCCGCAGCA
TCTTCCCTGATAAACAGAAAATCCCGACCGTGACTTATTTGAAAAGACAGATGAGTGATGAGATGGCGCGTTTATCTGGC
GGTGCCAGTGTGGGCAGCGTTTTGAAGTGGCTAAGCCCGTTGCCTGAGGCTTTGAAAGGGGTCAATCTACAACTGCAAAG
CATCAAATTTGACAGTAACCGCAGTGAGATTCGCCTAGAAGCGACCAGTCGTGATTTCCAAAGTTTTGAACAAGCTCGCA
CTCAGCTTGAGCAGTATTTTGCTGTTGAACAGGGGCAGCTCAATAAAAATGGCGAGCAAGTGTTTGGCGTGTTTGTGGTG
AAGCCCAAGTAA

Upstream 100 bases:

>100_bases
CAGGTGCTGGTCGATACGTCTCGCGTGCGGATCCGCAGCCTGTTTTACAGTAACGATAAGAAAACTGCGACGGTGATACG
CCGCCGCTTTGGAGGGATCA

Downstream 100 bases:

>100_bases
GGAGAAATGATGAAAGAATTATTGGCTCCTGTGCAGGCTTGGTGGCGAAGTGTCACCCCTCGTGAGCAAAAGATGGTAAT
GGGCATGGGCGCGCTGACGG

Product: general secretion pathway protein L

Products: NA

Alternate protein names: Cholera toxin secretion protein epsL [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSEFLTVRLSSQKEADIPWLVWSAEQQEVIASGQVAGWEALHEIESYADQRSVVVLLAASDLILTSVEIPPGASRQLENM
LPYLLEDEIAQDVEDVHFCVLSKGRETADVVGVDRLWLRACLDHLKACGFDVKRVLPDVLAIPRPEHGLAALQLGDEWLV
RKSTTQGMAVDAQWLSLLAASDWVQNEGEYLPLQALTPLPELSLAETQEWRYEPSGLVMQLLTQEALTSKFNLLTGSFKL
KSSWLRYWQIWRKVAIAAGLFVAVSISYSLFQAHQYEAQADAYRAESERIFRSIFPDKQKIPTVTYLKRQMSDEMARLSG
GASVGSVLKWLSPLPEALKGVNLQLQSIKFDSNRSEIRLEATSRDFQSFEQARTQLEQYFAVEQGQLNKNGEQVFGVFVV
KPK

Sequences:

>Translated_403_residues
MSEFLTVRLSSQKEADIPWLVWSAEQQEVIASGQVAGWEALHEIESYADQRSVVVLLAASDLILTSVEIPPGASRQLENM
LPYLLEDEIAQDVEDVHFCVLSKGRETADVVGVDRLWLRACLDHLKACGFDVKRVLPDVLAIPRPEHGLAALQLGDEWLV
RKSTTQGMAVDAQWLSLLAASDWVQNEGEYLPLQALTPLPELSLAETQEWRYEPSGLVMQLLTQEALTSKFNLLTGSFKL
KSSWLRYWQIWRKVAIAAGLFVAVSISYSLFQAHQYEAQADAYRAESERIFRSIFPDKQKIPTVTYLKRQMSDEMARLSG
GASVGSVLKWLSPLPEALKGVNLQLQSIKFDSNRSEIRLEATSRDFQSFEQARTQLEQYFAVEQGQLNKNGEQVFGVFVV
KPK
>Mature_402_residues
SEFLTVRLSSQKEADIPWLVWSAEQQEVIASGQVAGWEALHEIESYADQRSVVVLLAASDLILTSVEIPPGASRQLENML
PYLLEDEIAQDVEDVHFCVLSKGRETADVVGVDRLWLRACLDHLKACGFDVKRVLPDVLAIPRPEHGLAALQLGDEWLVR
KSTTQGMAVDAQWLSLLAASDWVQNEGEYLPLQALTPLPELSLAETQEWRYEPSGLVMQLLTQEALTSKFNLLTGSFKLK
SSWLRYWQIWRKVAIAAGLFVAVSISYSLFQAHQYEAQADAYRAESERIFRSIFPDKQKIPTVTYLKRQMSDEMARLSGG
ASVGSVLKWLSPLPEALKGVNLQLQSIKFDSNRSEIRLEATSRDFQSFEQARTQLEQYFAVEQGQLNKNGEQVFGVFVVK
PK

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3297

COG function: function code U; Type II secretory pathway, component PulL

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP L family [H]

Homologues:

Organism=Escherichia coli, GI87082244, Length=140, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007812 [H]

Pfam domain/function: PF05134 GspL [H]

EC number: NA

Molecular weight: Translated: 45377; Mature: 45246

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFLTVRLSSQKEADIPWLVWSAEQQEVIASGQVAGWEALHEIESYADQRSVVVLLAAS
CCCEEEEEECCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEC
DLILTSVEIPPGASRQLENMLPYLLEDEIAQDVEDVHFCVLSKGRETADVVGVDRLWLRA
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
CLDHLKACGFDVKRVLPDVLAIPRPEHGLAALQLGDEWLVRKSTTQGMAVDAQWLSLLAA
HHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHEECCCCCCCEEHHHHHHHHHH
SDWVQNEGEYLPLQALTPLPELSLAETQEWRYEPSGLVMQLLTQEALTSKFNLLTGSFKL
HHHHCCCCCEECHHHCCCCCCCCHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
KSSWLRYWQIWRKVAIAAGLFVAVSISYSLFQAHQYEAQADAYRAESERIFRSIFPDKQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IPTVTYLKRQMSDEMARLSGGASVGSVLKWLSPLPEALKGVNLQLQSIKFDSNRSEIRLE
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
ATSRDFQSFEQARTQLEQYFAVEQGQLNKNGEQVFGVFVVKPK
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
SEFLTVRLSSQKEADIPWLVWSAEQQEVIASGQVAGWEALHEIESYADQRSVVVLLAAS
CCEEEEEECCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEC
DLILTSVEIPPGASRQLENMLPYLLEDEIAQDVEDVHFCVLSKGRETADVVGVDRLWLRA
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
CLDHLKACGFDVKRVLPDVLAIPRPEHGLAALQLGDEWLVRKSTTQGMAVDAQWLSLLAA
HHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHEECCCCCCCEEHHHHHHHHHH
SDWVQNEGEYLPLQALTPLPELSLAETQEWRYEPSGLVMQLLTQEALTSKFNLLTGSFKL
HHHHCCCCCEECHHHCCCCCCCCHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
KSSWLRYWQIWRKVAIAAGLFVAVSISYSLFQAHQYEAQADAYRAESERIFRSIFPDKQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IPTVTYLKRQMSDEMARLSGGASVGSVLKWLSPLPEALKGVNLQLQSIKFDSNRSEIRLE
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
ATSRDFQSFEQARTQLEQYFAVEQGQLNKNGEQVFGVFVVKPK
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]