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The map label for this gene is ldhA [C]

Identifier: 147673560

GI number: 147673560

Start: 2244805

End: 2245782

Strand: Reverse

Name: ldhA [C]

Synonym: VC0395_A2086

Alternate gene names: 147673560

Gene position: 2245782-2244805 (Counterclockwise)

Preceding gene: 147673149

Following gene: 147673657

Centisome position: 74.26

GC content: 51.02

Gene sequence:

>978_bases
ATGTCACTACCCACTACTTCTTTGCCTACGGTCGTTTTTCTCGACCGCGCGACCATTCCGCGCCATATCCCCTTACCTGC
TCTGCCTTTTGAACACCATTGGGTTGAGTATGATGCTTGTGAGCCTCACCAAGTGGTTGAGCGCCTCTTAGCGGCCGATA
TCGTTATCACTAATAAGGTGGTACTCACCCGTGAAATGCTGGTTCAGTTGCCTAAGCTCAAGTTGATCGCCATTTCTGCC
ACGGGCACCAACAACGTCGATTTGCAGGCGTGTAGTGACCTGAATATCGCGGTGTGTAATGTGCAAGGCTATGCCACGCG
CTCCGTACCAGAGCATGTGATCGCCATGATGTTTGCTCTAAGACGTAATCTTATCGGTTATCACAATGATATTGCGGCCG
GAGAGTGGCAGCGCCATAAGCAGTTCTGTTTTTTCACGCATCCTATCGGCGATATTGCGGGCTCCACCATGGGCATTATC
GGCAGTGGTGCATTAGGACAAGCGACGGCGAATTTAGCGCGCGCATTAGGTATGCATGTGCTGTTTGCTGAACGTAAAGG
TCAGTTGGAATGCCGAGTAGGGTACACGTCATTTGAACAAGTGCTGGCGCAATCCGATGTGTTATCCCTGCACTGTCCGC
TAATGGATGAAACGCGCAATATTATCTCGGAAGCGGAATTGGTGCAGATGAAGCCCAATGCTCTACTGATCAATACTGGG
CGCGGTGGTTTAGTGGATGAACAGGCGCTGGTAGATGCGCTGAAAAGACGTCAAATTGCTGGGGCTGGGGTGGATGTGTT
TAGTGTCGAGCCTGCGGATATGGATAACCCACTGATTGCCAACCGTGATCTGCCCAATCTTCTGCTGACACCGCATGTGG
CGTGGGGCAGTGATTCCTCGATACAACAACTCGCGACTATCTTGATCGACAACATCAGTGCATTTATGCGTGGTGAAGCG
AAAAATCGAGTCGTCTAG

Upstream 100 bases:

>100_bases
TTTGCAGCATTTCGATCCTGAGCGAAAATTACCGGACTATAAAAAGCTATCCCAAGACTAATATTATTGTGGGTAGCGGA
TAATCAGACCCGGAGTCAGC

Downstream 100 bases:

>100_bases
CGGCAACATCATCGCTAAAACAAAAAGGCAGCGAAATCGCTGCCTTTTTTATCGACTTTTTGTATGGAATGCTTGGCTTA
CAGCGCAGCGATGGTCGCTT

Product: 2-hydroxyacid dehydrogenase family protein

Products: H+; Nicotinamide adenine dinucleotide - reduced; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSLPTTSLPTVVFLDRATIPRHIPLPALPFEHHWVEYDACEPHQVVERLLAADIVITNKVVLTREMLVQLPKLKLIAISA
TGTNNVDLQACSDLNIAVCNVQGYATRSVPEHVIAMMFALRRNLIGYHNDIAAGEWQRHKQFCFFTHPIGDIAGSTMGII
GSGALGQATANLARALGMHVLFAERKGQLECRVGYTSFEQVLAQSDVLSLHCPLMDETRNIISEAELVQMKPNALLINTG
RGGLVDEQALVDALKRRQIAGAGVDVFSVEPADMDNPLIANRDLPNLLLTPHVAWGSDSSIQQLATILIDNISAFMRGEA
KNRVV

Sequences:

>Translated_325_residues
MSLPTTSLPTVVFLDRATIPRHIPLPALPFEHHWVEYDACEPHQVVERLLAADIVITNKVVLTREMLVQLPKLKLIAISA
TGTNNVDLQACSDLNIAVCNVQGYATRSVPEHVIAMMFALRRNLIGYHNDIAAGEWQRHKQFCFFTHPIGDIAGSTMGII
GSGALGQATANLARALGMHVLFAERKGQLECRVGYTSFEQVLAQSDVLSLHCPLMDETRNIISEAELVQMKPNALLINTG
RGGLVDEQALVDALKRRQIAGAGVDVFSVEPADMDNPLIANRDLPNLLLTPHVAWGSDSSIQQLATILIDNISAFMRGEA
KNRVV
>Mature_324_residues
SLPTTSLPTVVFLDRATIPRHIPLPALPFEHHWVEYDACEPHQVVERLLAADIVITNKVVLTREMLVQLPKLKLIAISAT
GTNNVDLQACSDLNIAVCNVQGYATRSVPEHVIAMMFALRRNLIGYHNDIAAGEWQRHKQFCFFTHPIGDIAGSTMGIIG
SGALGQATANLARALGMHVLFAERKGQLECRVGYTSFEQVLAQSDVLSLHCPLMDETRNIISEAELVQMKPNALLINTGR
GGLVDEQALVDALKRRQIAGAGVDVFSVEPADMDNPLIANRDLPNLLLTPHVAWGSDSSIQQLATILIDNISAFMRGEAK
NRVV

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=273, Percent_Identity=36.2637362637363, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4557499, Length=273, Percent_Identity=36.2637362637363, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4557497, Length=275, Percent_Identity=35.2727272727273, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI61743967, Length=275, Percent_Identity=35.2727272727273, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI145580575, Length=273, Percent_Identity=36.2637362637363, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI23308577, Length=245, Percent_Identity=27.7551020408163, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI6912396, Length=252, Percent_Identity=26.984126984127, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1787645, Length=274, Percent_Identity=32.8467153284672, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082289, Length=271, Percent_Identity=28.7822878228782, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789279, Length=269, Percent_Identity=34.9442379182156, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=316, Percent_Identity=29.4303797468354, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17532191, Length=270, Percent_Identity=29.6296296296296, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=258, Percent_Identity=32.5581395348837, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=258, Percent_Identity=32.1705426356589, Blast_Score=125, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324964, Length=290, Percent_Identity=28.9655172413793, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324055, Length=264, Percent_Identity=28.030303030303, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324980, Length=111, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325144, Length=145, Percent_Identity=29.6551724137931, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646446, Length=309, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646448, Length=309, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646452, Length=309, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646450, Length=309, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI62472511, Length=275, Percent_Identity=34.1818181818182, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI28571528, Length=254, Percent_Identity=28.740157480315, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28574286, Length=286, Percent_Identity=28.3216783216783, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19921140, Length=238, Percent_Identity=26.890756302521, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585516, Length=252, Percent_Identity=25.3968253968254, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585514, Length=255, Percent_Identity=25.4901960784314, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28574282, Length=255, Percent_Identity=25.4901960784314, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI45552429, Length=255, Percent_Identity=25.4901960784314, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45551003, Length=255, Percent_Identity=25.4901960784314, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28574284, Length=255, Percent_Identity=25.4901960784314, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28 [C]

Molecular weight: Translated: 35551; Mature: 35419

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPTTSLPTVVFLDRATIPRHIPLPALPFEHHWVEYDACEPHQVVERLLAADIVITNKV
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHEECHH
VLTREMLVQLPKLKLIAISATGTNNVDLQACSDLNIAVCNVQGYATRSVPEHVIAMMFAL
HHHHHHHHHCCCEEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHH
RRNLIGYHNDIAAGEWQRHKQFCFFTHPIGDIAGSTMGIIGSGALGQATANLARALGMHV
HHHHCCCCCCCCCCCHHHCCCEEEEECCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHE
LFAERKGQLECRVGYTSFEQVLAQSDVLSLHCPLMDETRNIISEAELVQMKPNALLINTG
EEECCCCCEEEEECCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHEEECCCEEEEECC
RGGLVDEQALVDALKRRQIAGAGVDVFSVEPADMDNPLIANRDLPNLLLTPHVAWGSDSS
CCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCEEECCEECCCCCHH
IQQLATILIDNISAFMRGEAKNRVV
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLPTTSLPTVVFLDRATIPRHIPLPALPFEHHWVEYDACEPHQVVERLLAADIVITNKV
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHEECHH
VLTREMLVQLPKLKLIAISATGTNNVDLQACSDLNIAVCNVQGYATRSVPEHVIAMMFAL
HHHHHHHHHCCCEEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHH
RRNLIGYHNDIAAGEWQRHKQFCFFTHPIGDIAGSTMGIIGSGALGQATANLARALGMHV
HHHHCCCCCCCCCCCHHHCCCEEEEECCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHE
LFAERKGQLECRVGYTSFEQVLAQSDVLSLHCPLMDETRNIISEAELVQMKPNALLINTG
EEECCCCCEEEEECCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHEEECCCEEEEECC
RGGLVDEQALVDALKRRQIAGAGVDVFSVEPADMDNPLIANRDLPNLLLTPHVAWGSDSS
CCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCEEECCEECCCCCHH
IQQLATILIDNISAFMRGEAKNRVV
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Lactate; Nicotinamide adenine dinucleotide [C]

Specific reaction: D-Lactate + Nicotinamide adenine dinucleotide <==> H+ + Nicotinamide adenine dinucleotide - reduced + Pyruvate [C]

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]