Click here to switch to the map view.

The map label for this gene is recA

Identifier: 147673530

GI number: 147673530

Start: 65482

End: 66546

Strand: Direct

Name: recA

Synonym: VC0395_A0070

Alternate gene names: 147673530

Gene position: 65482-66546 (Clockwise)

Preceding gene: 147673308

Following gene: 147674135

Centisome position: 2.17

GC content: 48.08

Gene sequence:

>1065_bases
ATGGACGAGAATAAACAGAAGGCACTTGCGGCCGCCCTCGGTCAAATTGAAAAGCAATTTGGTAAAGGCTCCATCATGCG
CTTAGGCGACAACCGCGCGATGGATGTAGAAACCATTTCGACCGGTTCTCTGTCTCTGGATATCGCGTTGGGTGCTGGTG
GCCTACCAATGGGACGTATCGTTGAGATCTTCGGCCCTGAATCTTCGGGTAAAACGACACTGACTCTGGAACTGATTGCT
GCTGCGCAACGTGAAGGTAAAACCTGTGCGTTTATCGATGCCGAGCACGCGTTGGATCCGGTTTATGCCAAGAAATTGGG
CGTGAATATCGATGAGCTGCTGGTTTCTCAGCCTGATACCGGCGAGCAAGCACTGGAAATTTGTGATGCACTGGCTCGCT
CTGGTGCGGTAGATGTGATTGTTGTTGACTCAGTCGCGGCTTTGACACCAAAAGCGGAAATCGAAGGCGAAATGGGCGAT
AGCCACATGGGTCTTCAAGCGCGTATGTTGTCGCAAGCGATGCGTAAACTGACGGGTAACCTCAAGCAATCCAACTGTAT
GTGTATCTTCATCAACCAAATTCGTATGAAGATTGGTGTGATGTTTGGTAACCCAGAAACCACCACTGGCGGTAACGCAC
TGAAATTCTACGCTTCTGTTCGTTTGGATATTCGCCGTACTGGCGCAATCAAAGAAGGCGAAGAAGTGGTTGGTAACGAA
ACCCGTATCAAGGTTGTGAAGAACAAGATTGCGGCACCGTTTAAAGAAGCCAACACTCAGATCATGTACGGCCAAGGTTT
CAACCGTGAAGGTGAATTGATCGATCTTGGTGTGAAACACAAGATGGTTGAAAAGTCAGGTGCTTGGTACAGCTACAACG
GCGATAAAATCGGCCAAGGTAAAGCGAATGCCTGCAAGTATCTGAAAGAAAATCCAGAGATTGCTAAGACGCTAGACAAA
AAACTACGTGAAATGCTGCTTAACCCAGAAAATATGCAACTGACTGGTGAAACGGCTTCTGCCGCTGATGATGTAGAATT
TGGTGCAGTGCCAGAAGAGTTTTAA

Upstream 100 bases:

>100_bases
TACAAATCAACAGTATAATGAGCCCCAACATGACAAATCAGCGTCGACCCGATTTCGACACAATTACCTATCACCTGAGA
GTGACTATCCGGAGAGAGTA

Downstream 100 bases:

>100_bases
TCGGCAGGCTGAATGCAAAGAGGTTCAACCTCTCAATCTCGATGTGAATGAAGCCCTGCAATGCAGGGCTTCTTTTTGGC
TGCTAATTGAGTGAGTGACT

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MDENKQKALAAALGQIEKQFGKGSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIA
AAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGD
SHMGLQARMLSQAMRKLTGNLKQSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNE
TRIKVVKNKIAAPFKEANTQIMYGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKANACKYLKENPEIAKTLDK
KLREMLLNPENMQLTGETASAADDVEFGAVPEEF

Sequences:

>Translated_354_residues
MDENKQKALAAALGQIEKQFGKGSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIA
AAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGD
SHMGLQARMLSQAMRKLTGNLKQSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNE
TRIKVVKNKIAAPFKEANTQIMYGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKANACKYLKENPEIAKTLDK
KLREMLLNPENMQLTGETASAADDVEFGAVPEEF
>Mature_354_residues
MDENKQKALAAALGQIEKQFGKGSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRIVEIFGPESSGKTTLTLELIA
AAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGD
SHMGLQARMLSQAMRKLTGNLKQSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNE
TRIKVVKNKIAAPFKEANTQIMYGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQGKANACKYLKENPEIAKTLDK
KLREMLLNPENMQLTGETASAADDVEFGAVPEEF

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family

Homologues:

Organism=Escherichia coli, GI1789051, Length=331, Percent_Identity=84.2900302114804, Blast_Score=581, Evalue=1e-167,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RECA_VIBC3 (A5F9B5)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F9B5
- SMR:   A5F9B5
- STRING:   A5F9B5
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0070
- eggNOG:   COG0468
- HOGENOM:   HBG339889
- OMA:   YGEGINT
- ProtClustDB:   PRK09354
- BioCyc:   VCHO345073:VC0395_A0070-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00268
- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587
- PANTHER:   PTHR22942:SF1
- PRINTS:   PR00142
- SMART:   SM00382
- TIGRFAMs:   TIGR02012

Pfam domain/function: PF00154 RecA

EC number: NA

Molecular weight: Translated: 38208; Mature: 38208

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00321 RECA_1; PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDENKQKALAAALGQIEKQFGKGSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRI
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCEEEEEEECCCCCCHHHE
VEIFGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDT
EEEECCCCCCCEEEEEEEEEHHHCCCCEEEEEEHHHCCCHHHHHHHCCCHHHHHCCCCCC
GEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGN
HHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
LKQSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNE
CCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHCCC
TRIKVVKNKIAAPFKEANTQIMYGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQG
CEEEEEHHHHCCCHHHCCCEEEECCCCCCCCCEEEECCHHHHHHCCCCEEEECCCCCCCC
KANACKYLKENPEIAKTLDKKLREMLLNPENMQLTGETASAADDVEFGAVPEEF
CHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDENKQKALAAALGQIEKQFGKGSIMRLGDNRAMDVETISTGSLSLDIALGAGGLPMGRI
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCEEEEEEECCCCCCHHHE
VEIFGPESSGKTTLTLELIAAAQREGKTCAFIDAEHALDPVYAKKLGVNIDELLVSQPDT
EEEECCCCCCCEEEEEEEEEHHHCCCCEEEEEEHHHCCCHHHHHHHCCCHHHHHCCCCCC
GEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEMGDSHMGLQARMLSQAMRKLTGN
HHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
LKQSNCMCIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVVGNE
CCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHCCC
TRIKVVKNKIAAPFKEANTQIMYGQGFNREGELIDLGVKHKMVEKSGAWYSYNGDKIGQG
CEEEEEHHHHCCCHHHCCCEEEECCCCCCCCCEEEECCHHHHHHCCCCEEEECCCCCCCC
KANACKYLKENPEIAKTLDKKLREMLLNPENMQLTGETASAADDVEFGAVPEEF
CHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA