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The map label for this gene is yjeA [H]

Identifier: 147673524

GI number: 147673524

Start: 2379346

End: 2380395

Strand: Reverse

Name: yjeA [H]

Synonym: VC0395_A2229

Alternate gene names: 147673524

Gene position: 2380395-2379346 (Counterclockwise)

Preceding gene: 147673907

Following gene: 147675092

Centisome position: 78.71

GC content: 48.67

Gene sequence:

>1050_bases
GTGTCACAATTGAGTTTGGCTTTTTGTTCTGAAAGAGTACAATTCGTTATCTCTGTTTCTTGTGATGAGCCACCCATGAC
TAATTCCGATTGGATGCCGACCGCATCAATAAGCCAATTAAAACAACGCGCGACCTTGTTGCGTCAGATTCGTGAATTTT
TTGCTGAGCGCAATGTGCTGGAAGTCGAAACGCCAGCCATGAGCCACGCCACGGTGACGGACATCCATCTGCACACGTTT
AAAACGGAATTTGTCGGGCCGGGTTATGCCAAAGGCAGCGCGCTGCATCTGATGACTAGCCCTGAATTTCATATGAAGCG
TTTGTTGGCCGCGGGCAGTGGTTGTATTTACCAGCTGGGTAAAGCTTTTCGTAACGAAGAAAATGGCCGTTACCACAATC
CAGAATTCACCATGTTGGAATGGTATCGGATTGGTTTTGATCACCATGCCTTGATGGATGAAATGGATGCCTTATTGCAA
TTGGTGCTGCGTTGCGGCAGTGCCGAGCGAATGACTTACCAAGAAGCGTTTTTGAATGTGCTTGGCGTTTGCCCACTCGA
AGAGGAGATGCGTGAGTTAAAGCAAGTGGCTGCAACGCTGGGTTTAAGTGATATCGCAGAGCCTGAAGAGGATCGTGACA
CCTTATTACAACTGCTGTTTAGCATTGGCATTGAGCCTAAAATTGGCCAAATCACACCAGCATTTGTGTATGATTTTCCG
GCTTCACAAGCGGCGCTGGCCAAAATTAATCCGGCGGATCCGCGCGTGGCTGATCGCTTCGAAGTCTATTTTAAAGGGAT
TGAGCTCGCGAACGGCTTCCATGAATTGGATAACCCTGCCGAGCAGTTAGCCCGTTTTAAAGCGGATAATGCCAAGCGTT
TAGAGATGGGACTGACAGAGCAACCGATTGATTACCATCTGATTGCGGCGCTGGAGGCCGGTTTGCCAGAATGTGCTGGG
GTGGCTTTGGGTATTGATCGTCTGATTATGTTGGCGCTTGGCGAGGATCATATTGATAAGGTCACCGCATTTCCATTTCC
TCGAGCCTGA

Upstream 100 bases:

>100_bases
GGGGTAGTGTAGGAGAGCTTGAAGAATGAAAAATTGATTCTGTTGGGCTTTTGCTTCGGTATTGCAAACTTAAGCATACT
ATTTGCCCTATATGTGACTG

Downstream 100 bases:

>100_bases
TCGGATTCTCAGTTTGCAACATATTCACCCTGTTCAGACCCAGTTTTAACGAGGTAAGCGCTCGCGTCATGCTGCCAACC
TCGTTATACTCCTTGTCTGA

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSQLSLAFCSERVQFVISVSCDEPPMTNSDWMPTASISQLKQRATLLRQIREFFAERNVLEVETPAMSHATVTDIHLHTF
KTEFVGPGYAKGSALHLMTSPEFHMKRLLAAGSGCIYQLGKAFRNEENGRYHNPEFTMLEWYRIGFDHHALMDEMDALLQ
LVLRCGSAERMTYQEAFLNVLGVCPLEEEMRELKQVAATLGLSDIAEPEEDRDTLLQLLFSIGIEPKIGQITPAFVYDFP
ASQAALAKINPADPRVADRFEVYFKGIELANGFHELDNPAEQLARFKADNAKRLEMGLTEQPIDYHLIAALEAGLPECAG
VALGIDRLIMLALGEDHIDKVTAFPFPRA

Sequences:

>Translated_349_residues
MSQLSLAFCSERVQFVISVSCDEPPMTNSDWMPTASISQLKQRATLLRQIREFFAERNVLEVETPAMSHATVTDIHLHTF
KTEFVGPGYAKGSALHLMTSPEFHMKRLLAAGSGCIYQLGKAFRNEENGRYHNPEFTMLEWYRIGFDHHALMDEMDALLQ
LVLRCGSAERMTYQEAFLNVLGVCPLEEEMRELKQVAATLGLSDIAEPEEDRDTLLQLLFSIGIEPKIGQITPAFVYDFP
ASQAALAKINPADPRVADRFEVYFKGIELANGFHELDNPAEQLARFKADNAKRLEMGLTEQPIDYHLIAALEAGLPECAG
VALGIDRLIMLALGEDHIDKVTAFPFPRA
>Mature_348_residues
SQLSLAFCSERVQFVISVSCDEPPMTNSDWMPTASISQLKQRATLLRQIREFFAERNVLEVETPAMSHATVTDIHLHTFK
TEFVGPGYAKGSALHLMTSPEFHMKRLLAAGSGCIYQLGKAFRNEENGRYHNPEFTMLEWYRIGFDHHALMDEMDALLQL
VLRCGSAERMTYQEAFLNVLGVCPLEEEMRELKQVAATLGLSDIAEPEEDRDTLLQLLFSIGIEPKIGQITPAFVYDFPA
SQAALAKINPADPRVADRFEVYFKGIELANGFHELDNPAEQLARFKADNAKRLEMGLTEQPIDYHLIAALEAGLPECAGV
ALGIDRLIMLALGEDHIDKVTAFPFPRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=331, Percent_Identity=28.0966767371601, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI5031815, Length=331, Percent_Identity=28.0966767371601, Blast_Score=114, Evalue=9e-26,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=60.5590062111801, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1790571, Length=329, Percent_Identity=32.2188449848024, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1789256, Length=336, Percent_Identity=30.952380952381, Blast_Score=132, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994340, Length=333, Percent_Identity=27.9279279279279, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535927, Length=333, Percent_Identity=27.9279279279279, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535925, Length=332, Percent_Identity=28.3132530120482, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320242, Length=335, Percent_Identity=28.3582089552239, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324256, Length=346, Percent_Identity=27.7456647398844, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24640851, Length=332, Percent_Identity=27.1084337349398, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24640849, Length=332, Percent_Identity=27.1084337349398, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 39077; Mature: 38946

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLSLAFCSERVQFVISVSCDEPPMTNSDWMPTASISQLKQRATLLRQIREFFAERNVL
CCCCHHHHHHHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
EVETPAMSHATVTDIHLHTFKTEFVGPGYAKGSALHLMTSPEFHMKRLLAAGSGCIYQLG
EEECCCCCCCEEEEEEEEEEHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHH
KAFRNEENGRYHNPEFTMLEWYRIGFDHHALMDEMDALLQLVLRCGSAERMTYQEAFLNV
HHHCCCCCCCEECCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LGVCPLEEEMRELKQVAATLGLSDIAEPEEDRDTLLQLLFSIGIEPKIGQITPAFVYDFP
HCCCCCHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHEEECC
ASQAALAKINPADPRVADRFEVYFKGIELANGFHELDNPAEQLARFKADNAKRLEMGLTE
CCCHHHEECCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCHHHHCCCCC
QPIDYHLIAALEAGLPECAGVALGIDRLIMLALGEDHIDKVTAFPFPRA
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
SQLSLAFCSERVQFVISVSCDEPPMTNSDWMPTASISQLKQRATLLRQIREFFAERNVL
CCCHHHHHHHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
EVETPAMSHATVTDIHLHTFKTEFVGPGYAKGSALHLMTSPEFHMKRLLAAGSGCIYQLG
EEECCCCCCCEEEEEEEEEEHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHH
KAFRNEENGRYHNPEFTMLEWYRIGFDHHALMDEMDALLQLVLRCGSAERMTYQEAFLNV
HHHCCCCCCCEECCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LGVCPLEEEMRELKQVAATLGLSDIAEPEEDRDTLLQLLFSIGIEPKIGQITPAFVYDFP
HCCCCCHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHEEECC
ASQAALAKINPADPRVADRFEVYFKGIELANGFHELDNPAEQLARFKADNAKRLEMGLTE
CCCHHHEECCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCHHHHCCCCC
QPIDYHLIAALEAGLPECAGVALGIDRLIMLALGEDHIDKVTAFPFPRA
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]