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The map label for this gene is htrB [H]

Identifier: 147673430

GI number: 147673430

Start: 2746788

End: 2747744

Strand: Reverse

Name: htrB [H]

Synonym: VC0395_A2592

Alternate gene names: 147673430

Gene position: 2747744-2746788 (Counterclockwise)

Preceding gene: 147674218

Following gene: 147673251

Centisome position: 90.86

GC content: 49.63

Gene sequence:

>957_bases
GTGAGTACAATGAGTCAAGATAAATACAGCAAACCTGAATTCTCCTTCTCTTTATTGCACCCAAAATACTGGGGAGTCTG
GCTTGGTTTTGGCCTACTCGCCCTACTGGTTAACCTGCTGCCTTATCCTGTGCTGCTAAAAATCGGCCGCAGTTTAGGCC
AATTTTCGATGCGCTTTGGTAAAAAGCGTGTCCACATCGCCAGACGTAATCTGGAACTCGCCTTCCCAACCATGAGCCAA
AGCGAGATCGATGCGTTTGTCTTGGAAAACTTCAAAAACACCGGTGCGGCTCTCATTGAAACTGGGATCACTTGGTTCTG
GCCAACCTGGCGCTTTAAGCGCATTTTGATTGATAAAGATACCCAAGCCATTCGCCAACATGCACAAACAGGCAAAGGCG
TTTTGCTGTGTTGCGTACATGCACTCAATCTGGAAATCACCGCGCGCGCGTTTGCTGTGCTAGGCATCGGTGGCTATGGC
GTGTATCGTCCGCACAGTAATCCTGCCTATGAGTTCATTCAGTACCGTGGTCGAACACGCAATGGCAATCAGCTTATCAA
CCGAACCGATATTAAGCAGATGATCCGCGTGTTACGCCAAGGCGAGCGTCTGTTCTACCTGCCAGACCAAGACTATGGCC
ACAATAAGTCGGTGTTTGTGCCTTTCTTTGCGGTAGAAGAAGCTTGTACCACCACCGGCACCAGTATTTTGGCTTACACC
AGCCACTGCGCGATTGTCATCGGCTCCGGTTTCCGTAACGCGCAAGGGCGTTATGAGATCATGGCTGATAAATCAATTGA
AGCGGACTACCCACAAAAAGATGAAACCGCTGCCGCGGCTTACATGAATAAATTTGTCGAAGAGATTATACTGCGCGCCC
CCGAGCAATGGATGTGGCTGCACAAACGCTTCAAATCCTTGCCGGACTACGAACTGACCAACTCGCGCTATCAGTAA

Upstream 100 bases:

>100_bases
AGGTGGAAGGCAGCTTGAATCGCTTTGTGCGTTCTGATTTCAAATACCTGCCAACCGCGAATTTTGATGAATACTGGGCG
CTGCTAAGTGCACAGATTAA

Downstream 100 bases:

>100_bases
AGGTGAATTTGTGAGCGATCAAGACAACGGCATTGCACGCCATCTACATAACCCGCAATTTGAGTGGCGCTTTCTGCACC
CCAAACACTGGGGCACTTGG

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSTMSQDKYSKPEFSFSLLHPKYWGVWLGFGLLALLVNLLPYPVLLKIGRSLGQFSMRFGKKRVHIARRNLELAFPTMSQ
SEIDAFVLENFKNTGAALIETGITWFWPTWRFKRILIDKDTQAIRQHAQTGKGVLLCCVHALNLEITARAFAVLGIGGYG
VYRPHSNPAYEFIQYRGRTRNGNQLINRTDIKQMIRVLRQGERLFYLPDQDYGHNKSVFVPFFAVEEACTTTGTSILAYT
SHCAIVIGSGFRNAQGRYEIMADKSIEADYPQKDETAAAAYMNKFVEEIILRAPEQWMWLHKRFKSLPDYELTNSRYQ

Sequences:

>Translated_318_residues
MSTMSQDKYSKPEFSFSLLHPKYWGVWLGFGLLALLVNLLPYPVLLKIGRSLGQFSMRFGKKRVHIARRNLELAFPTMSQ
SEIDAFVLENFKNTGAALIETGITWFWPTWRFKRILIDKDTQAIRQHAQTGKGVLLCCVHALNLEITARAFAVLGIGGYG
VYRPHSNPAYEFIQYRGRTRNGNQLINRTDIKQMIRVLRQGERLFYLPDQDYGHNKSVFVPFFAVEEACTTTGTSILAYT
SHCAIVIGSGFRNAQGRYEIMADKSIEADYPQKDETAAAAYMNKFVEEIILRAPEQWMWLHKRFKSLPDYELTNSRYQ
>Mature_317_residues
STMSQDKYSKPEFSFSLLHPKYWGVWLGFGLLALLVNLLPYPVLLKIGRSLGQFSMRFGKKRVHIARRNLELAFPTMSQS
EIDAFVLENFKNTGAALIETGITWFWPTWRFKRILIDKDTQAIRQHAQTGKGVLLCCVHALNLEITARAFAVLGIGGYGV
YRPHSNPAYEFIQYRGRTRNGNQLINRTDIKQMIRVLRQGERLFYLPDQDYGHNKSVFVPFFAVEEACTTTGTSILAYTS
HCAIVIGSGFRNAQGRYEIMADKSIEADYPQKDETAAAAYMNKFVEEIILRAPEQWMWLHKRFKSLPDYELTNSRYQ

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=45.4248366013072, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI87082094, Length=303, Percent_Identity=40.5940594059406, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788161, Length=310, Percent_Identity=28.0645161290323, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 36573; Mature: 36441

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTMSQDKYSKPEFSFSLLHPKYWGVWLGFGLLALLVNLLPYPVLLKIGRSLGQFSMRFG
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
KKRVHIARRNLELAFPTMSQSEIDAFVLENFKNTGAALIETGITWFWPTWRFKRILIDKD
HHHHEEHHCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHEEEEEECCC
TQAIRQHAQTGKGVLLCCVHALNLEITARAFAVLGIGGYGVYRPHSNPAYEFIQYRGRTR
HHHHHHHHHCCCCHHHHHHHHHCCEEEEHEEEEEEECCCEEECCCCCHHHHHHHHCCCCC
NGNQLINRTDIKQMIRVLRQGERLFYLPDQDYGHNKSVFVPFFAVEEACTTTGTSILAYT
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEE
SHCAIVIGSGFRNAQGRYEIMADKSIEADYPQKDETAAAAYMNKFVEEIILRAPEQWMWL
CCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
HKRFKSLPDYELTNSRYQ
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
STMSQDKYSKPEFSFSLLHPKYWGVWLGFGLLALLVNLLPYPVLLKIGRSLGQFSMRFG
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
KKRVHIARRNLELAFPTMSQSEIDAFVLENFKNTGAALIETGITWFWPTWRFKRILIDKD
HHHHEEHHCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHEEEEEECCC
TQAIRQHAQTGKGVLLCCVHALNLEITARAFAVLGIGGYGVYRPHSNPAYEFIQYRGRTR
HHHHHHHHHCCCCHHHHHHHHHCCEEEEHEEEEEEECCCEEECCCCCHHHHHHHHCCCCC
NGNQLINRTDIKQMIRVLRQGERLFYLPDQDYGHNKSVFVPFFAVEEACTTTGTSILAYT
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEE
SHCAIVIGSGFRNAQGRYEIMADKSIEADYPQKDETAAAAYMNKFVEEIILRAPEQWMWL
CCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
HKRFKSLPDYELTNSRYQ
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]