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The map label for this gene is msbB [H]

Identifier: 147673251

GI number: 147673251

Start: 2745800

End: 2746777

Strand: Reverse

Name: msbB [H]

Synonym: VC0395_A2591

Alternate gene names: 147673251

Gene position: 2746777-2745800 (Counterclockwise)

Preceding gene: 147673430

Following gene: 147675681

Centisome position: 90.83

GC content: 53.07

Gene sequence:

>978_bases
GTGAGCGATCAAGACAACGGCATTGCACGCCATCTACATAACCCGCAATTTGAGTGGCGCTTTCTGCACCCCAAACACTG
GGGCACTTGGATTGGCATCGCTTTCGCAGCACTGTTAGCCTTTATTCCTTGGCGCTTGCGTGATCGACTGGCGAAACCTT
TGGTGCCACTCATCATCAAGAAAAATGGCCGCGTAGTACGCCGCGCTCGGGTTAACCTCGCCTACTGTTTTCCAGAGAAA
AGCGAGCAAGAGCGCGAGCAGATCCTGCACGATACGTTTGTGAAAGCCTCGCAGTTTATGCTCGGTTATTCAGAGCTGTT
GGTGCGCTCGACCCGCTACAACAACGCACGGGGCGAACTGATTGGCGAAGAGAACTTGCTGCCACTGCTGGATAGCGGTG
AGCGCGTGATTTTGTTAGTCCCGCACTCTTGGGCCATTGATTACGCCGCCGTTATGCTGGCGGCTCGCGGCTACAAAGTC
GCCAACATCATGAAACCGCAGCGCAACCCGATTGCCGATTGGCTGATGCACGTCCAACGTATGCAGTATGGTGGCCGTAT
TTTCACTCGTGAATCGGGCATCAAACCTTTCTTGCGTTCCATTCAATCCGGCTATGTTGGCTACTGGGTACCGGATGAAG
ATCACGGCCCGCAAAACTCCGTGTTTGTGCCGTTTTTTGCTACCGAAAAAGCCACGCTCAAAGGGTTTGGCAAAATGGCT
AAGCTGTGCAAAGCACACGTGGTGCCTTTGATGTCATGCTACAACAGTGATTCAGGCCGTTATGAAGTGCACATTTTGCC
TGCGCTGCAAAACTTCCCAACCGGCGATGAAGAAGCCGATGCACTGGCGATGAACCGTGCGATTGAAGCGCTGGTCACGC
CACAGCCGGAGCAGTACATGTGGAACTTGTCGTTGCTGAAAACTCAGCGTGATGGACGAGAGATTTACGACAACTCCCAT
CATGGCGATCAAAGCTAA

Upstream 100 bases:

>100_bases
ATACTGCGCGCCCCCGAGCAATGGATGTGGCTGCACAAACGCTTCAAATCCTTGCCGGACTACGAACTGACCAACTCGCG
CTATCAGTAAAGGTGAATTT

Downstream 100 bases:

>100_bases
GTGTTTTGTTCTTGAGCTTAATGCTAGAAAAACAGCCTCATTTTGAGGCTGTTTTTTCGTTTTAGGCTTAGTTGGATGAA
TACGCTTAAATTCCGTACTG

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSDQDNGIARHLHNPQFEWRFLHPKHWGTWIGIAFAALLAFIPWRLRDRLAKPLVPLIIKKNGRVVRRARVNLAYCFPEK
SEQEREQILHDTFVKASQFMLGYSELLVRSTRYNNARGELIGEENLLPLLDSGERVILLVPHSWAIDYAAVMLAARGYKV
ANIMKPQRNPIADWLMHVQRMQYGGRIFTRESGIKPFLRSIQSGYVGYWVPDEDHGPQNSVFVPFFATEKATLKGFGKMA
KLCKAHVVPLMSCYNSDSGRYEVHILPALQNFPTGDEEADALAMNRAIEALVTPQPEQYMWNLSLLKTQRDGREIYDNSH
HGDQS

Sequences:

>Translated_325_residues
MSDQDNGIARHLHNPQFEWRFLHPKHWGTWIGIAFAALLAFIPWRLRDRLAKPLVPLIIKKNGRVVRRARVNLAYCFPEK
SEQEREQILHDTFVKASQFMLGYSELLVRSTRYNNARGELIGEENLLPLLDSGERVILLVPHSWAIDYAAVMLAARGYKV
ANIMKPQRNPIADWLMHVQRMQYGGRIFTRESGIKPFLRSIQSGYVGYWVPDEDHGPQNSVFVPFFATEKATLKGFGKMA
KLCKAHVVPLMSCYNSDSGRYEVHILPALQNFPTGDEEADALAMNRAIEALVTPQPEQYMWNLSLLKTQRDGREIYDNSH
HGDQS
>Mature_324_residues
SDQDNGIARHLHNPQFEWRFLHPKHWGTWIGIAFAALLAFIPWRLRDRLAKPLVPLIIKKNGRVVRRARVNLAYCFPEKS
EQEREQILHDTFVKASQFMLGYSELLVRSTRYNNARGELIGEENLLPLLDSGERVILLVPHSWAIDYAAVMLAARGYKVA
NIMKPQRNPIADWLMHVQRMQYGGRIFTRESGIKPFLRSIQSGYVGYWVPDEDHGPQNSVFVPFFATEKATLKGFGKMAK
LCKAHVVPLMSCYNSDSGRYEVHILPALQNFPTGDEEADALAMNRAIEALVTPQPEQYMWNLSLLKTQRDGREIYDNSHH
GDQS

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=302, Percent_Identity=40.7284768211921, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1787292, Length=303, Percent_Identity=31.023102310231, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87082094, Length=302, Percent_Identity=25.1655629139073, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 37285; Mature: 37154

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQDNGIARHLHNPQFEWRFLHPKHWGTWIGIAFAALLAFIPWRLRDRLAKPLVPLIIK
CCCCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEC
KNGRVVRRARVNLAYCFPEKSEQEREQILHDTFVKASQFMLGYSELLVRSTRYNNARGEL
CCCCEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IGEENLLPLLDSGERVILLVPHSWAIDYAAVMLAARGYKVANIMKPQRNPIADWLMHVQR
CCCCCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHH
MQYGGRIFTRESGIKPFLRSIQSGYVGYWVPDEDHGPQNSVFVPFFATEKATLKGFGKMA
HHHCCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHH
KLCKAHVVPLMSCYNSDSGRYEVHILPALQNFPTGDEEADALAMNRAIEALVTPQPEQYM
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHE
WNLSLLKTQRDGREIYDNSHHGDQS
EEHHHHHCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
SDQDNGIARHLHNPQFEWRFLHPKHWGTWIGIAFAALLAFIPWRLRDRLAKPLVPLIIK
CCCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEC
KNGRVVRRARVNLAYCFPEKSEQEREQILHDTFVKASQFMLGYSELLVRSTRYNNARGEL
CCCCEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IGEENLLPLLDSGERVILLVPHSWAIDYAAVMLAARGYKVANIMKPQRNPIADWLMHVQR
CCCCCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHH
MQYGGRIFTRESGIKPFLRSIQSGYVGYWVPDEDHGPQNSVFVPFFATEKATLKGFGKMA
HHHCCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHH
KLCKAHVVPLMSCYNSDSGRYEVHILPALQNFPTGDEEADALAMNRAIEALVTPQPEQYM
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHE
WNLSLLKTQRDGREIYDNSHHGDQS
EEHHHHHCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]