Click here to switch to the map view.

The map label for this gene is uvrB [H]

Identifier: 147673403

GI number: 147673403

Start: 578523

End: 580553

Strand: Direct

Name: uvrB [H]

Synonym: VC0395_A0540

Alternate gene names: 147673403

Gene position: 578523-580553 (Clockwise)

Preceding gene: 147674163

Following gene: 147673171

Centisome position: 19.13

GC content: 48.1

Gene sequence:

>2031_bases
ATGAGCAAAGCATTTGAATTGGTTTCAGACTATCAACCTGCTGGTGATCAGCCTACCGCGATTAAGCAATTGCTGGAGGG
GTTGGAAGCGGGCTTGGCTCATCAAACTCTGCTTGGGGTAACGGGTTCAGGTAAAACCTTTACGTTAGCGAATGTGATTG
CCACCGCGCAGCGTCCCACCATTATACTTGCGCCTAATAAGACACTGGCGGCTCAATTGTACGGCGAAATGAAAGCCTTT
TTTCCCAACAATGCGGTGGAGTATTTCGTTTCTTACTACGACTACTATCAACCAGAAGCCTATGTGCCGACCACGGATAC
TTTTATTGAAAAAGACGCGTCCGTGAACGCTCATATTGAACAGATGCGCTTATCGGCCACTAAAGCCTTGCTTGAGCGCA
AAGATGCGGTGATTGTCGCTTCAGTTTCTGCCATATACGGTCTGGGCGATCCGGACTCTTATCTAAAAATGATGCTCCAT
TTACGTCGTGGTGATGTGATTAATCAACGTGACATGTTGCGTCGTTTGGCGGAGCTGCAATATTCACGCAATGATATGGC
ATTTGAGCGCGGTCAGTTCCGGGTACGCGGAGAAGTGATTGATATTTTCCCTGCCGAATCGGATCAAGAAGCTGTTCGAG
TGGAAATGTTTGATGATGAAATCGAATGCATCAGTTTATTTGATCCGCTGACCGGCGTGATTACGTCACGCGATTTAGCT
CGCTTTACGATTTACCCCAAAACTCACTATGTCACCCCACGCGAGCGCATTCTTGAGGCGATAGAGCAGATTAAGAGCGA
GTTGCAGGTCCGCCGCCAATATTTGCTGGATAACAATAAATTGCTGGAAGAGCAGCGGATTTCTCAGCGAACTCAGTTTG
ATATTGAGATGATGAACGAATTGGGATTCTGCTCAGGGATCGAAAACTACTCACGTTACCTAAGTGGTCGTGCAGAAGGC
GAGCCGCCTCCAACGCTCTTTGATTACTTGCCTCACGATGGCTTGCTGATCATCGATGAATCACACGTCACCGTGCCGCA
AATTGGCGCGATGTTTAAAGGCGATCGTTCACGAAAAGAAACGCTGGTGGAGTTCGGTTTTCGCCTACCTTCAGCCTTAG
ATAACCGCCCACTTAAGTTTGATGAATTTGAAGCATTAGCACCGCAAACCATCTTTGTGTCAGCAACACCGAGTGAATAT
GAGCTCACTAAATCGGGTGGTGAAGTGGCGGAGCAGGTGGTGCGTCCAACCGGTTTGTTAGACCCGATCATTGAAGTACG
TCCGGTCGCCACTCAGGTGGATGATCTGCTTTCTGAAGCGCGCATTCGCGCGGCCAATGATGAGCGGATTTTGGTGACTA
CCTTAACCAAACGCATGGCCGAGGACTTAACCGAATATCTGCATGAGCATGGTGTGAAAGTTCGCTATTTACACTCGGAC
ATTGATACCGTCGAACGGGTAGAAATTATTCGTGATTTACGATTAGGCGTATTTGATGTGTTGGTGGGGATCAACTTGCT
ACGTGAAGGTCTGGACATGCCAGAGGTCGCTTTAGTGGCGATTTTGGATGCTGATAAAGAAGGCTTTTTGCGTTCCGAGC
GTTCACTTATCCAGACCATGGGTCGTGCAGCACGTAACGTGAATGGTAAAGCAATTTTGTATGCCGACTCGATCACCAAA
TCCATGCGTAAAGCGATCGATGAAACCGAGCGTCGCCGTGAGAAGCAGCTGGCTTATAATGAGCAGCGCGGTATCACGCC
GCAGCCTTTAAAACGCAGCGTTAAAGACATCATGGAGCTGGGCGATATTGCGAAATCGCGCAAACAGAAGAACAGCAAAG
TGGTGCCATTGGCGAAAGTAGCTGAAGAATCGGCGGCTTACCAAGTGTTGACTCCTCAGCAGCTTGAAAAAGAGATCAGT
AAATTAGAAGCCGCAATGTATCAGCATGCGCAGAACTTAGAGTTTGAGTTAGCGGCGCAAAAACGTGATGAAATTCATCA
GTTGCGTCAGCAGTTTATCGCCAATAGTTAG

Upstream 100 bases:

>100_bases
ACCACGTTGTACGTGGCAAAAAGCGAGAGAAGAAGTGCTTGATCATCCGGTCAGCATCCCGCCACAATAGCGCTCAACAC
AAAAGCGTGACGAGGATGCA

Downstream 100 bases:

>100_bases
CCTTTCTTTCATTTTTAGAGGAAGCTGCGCGATAACACGACAGTGTTCGCCAATCATAGGTATCTTTATGTGAATTAGGG
ATAAATCAGGCGCCACTGAG

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B [H]

Number of amino acids: Translated: 676; Mature: 675

Protein sequence:

>676_residues
MSKAFELVSDYQPAGDQPTAIKQLLEGLEAGLAHQTLLGVTGSGKTFTLANVIATAQRPTIILAPNKTLAAQLYGEMKAF
FPNNAVEYFVSYYDYYQPEAYVPTTDTFIEKDASVNAHIEQMRLSATKALLERKDAVIVASVSAIYGLGDPDSYLKMMLH
LRRGDVINQRDMLRRLAELQYSRNDMAFERGQFRVRGEVIDIFPAESDQEAVRVEMFDDEIECISLFDPLTGVITSRDLA
RFTIYPKTHYVTPRERILEAIEQIKSELQVRRQYLLDNNKLLEEQRISQRTQFDIEMMNELGFCSGIENYSRYLSGRAEG
EPPPTLFDYLPHDGLLIIDESHVTVPQIGAMFKGDRSRKETLVEFGFRLPSALDNRPLKFDEFEALAPQTIFVSATPSEY
ELTKSGGEVAEQVVRPTGLLDPIIEVRPVATQVDDLLSEARIRAANDERILVTTLTKRMAEDLTEYLHEHGVKVRYLHSD
IDTVERVEIIRDLRLGVFDVLVGINLLREGLDMPEVALVAILDADKEGFLRSERSLIQTMGRAARNVNGKAILYADSITK
SMRKAIDETERRREKQLAYNEQRGITPQPLKRSVKDIMELGDIAKSRKQKNSKVVPLAKVAEESAAYQVLTPQQLEKEIS
KLEAAMYQHAQNLEFELAAQKRDEIHQLRQQFIANS

Sequences:

>Translated_676_residues
MSKAFELVSDYQPAGDQPTAIKQLLEGLEAGLAHQTLLGVTGSGKTFTLANVIATAQRPTIILAPNKTLAAQLYGEMKAF
FPNNAVEYFVSYYDYYQPEAYVPTTDTFIEKDASVNAHIEQMRLSATKALLERKDAVIVASVSAIYGLGDPDSYLKMMLH
LRRGDVINQRDMLRRLAELQYSRNDMAFERGQFRVRGEVIDIFPAESDQEAVRVEMFDDEIECISLFDPLTGVITSRDLA
RFTIYPKTHYVTPRERILEAIEQIKSELQVRRQYLLDNNKLLEEQRISQRTQFDIEMMNELGFCSGIENYSRYLSGRAEG
EPPPTLFDYLPHDGLLIIDESHVTVPQIGAMFKGDRSRKETLVEFGFRLPSALDNRPLKFDEFEALAPQTIFVSATPSEY
ELTKSGGEVAEQVVRPTGLLDPIIEVRPVATQVDDLLSEARIRAANDERILVTTLTKRMAEDLTEYLHEHGVKVRYLHSD
IDTVERVEIIRDLRLGVFDVLVGINLLREGLDMPEVALVAILDADKEGFLRSERSLIQTMGRAARNVNGKAILYADSITK
SMRKAIDETERRREKQLAYNEQRGITPQPLKRSVKDIMELGDIAKSRKQKNSKVVPLAKVAEESAAYQVLTPQQLEKEIS
KLEAAMYQHAQNLEFELAAQKRDEIHQLRQQFIANS
>Mature_675_residues
SKAFELVSDYQPAGDQPTAIKQLLEGLEAGLAHQTLLGVTGSGKTFTLANVIATAQRPTIILAPNKTLAAQLYGEMKAFF
PNNAVEYFVSYYDYYQPEAYVPTTDTFIEKDASVNAHIEQMRLSATKALLERKDAVIVASVSAIYGLGDPDSYLKMMLHL
RRGDVINQRDMLRRLAELQYSRNDMAFERGQFRVRGEVIDIFPAESDQEAVRVEMFDDEIECISLFDPLTGVITSRDLAR
FTIYPKTHYVTPRERILEAIEQIKSELQVRRQYLLDNNKLLEEQRISQRTQFDIEMMNELGFCSGIENYSRYLSGRAEGE
PPPTLFDYLPHDGLLIIDESHVTVPQIGAMFKGDRSRKETLVEFGFRLPSALDNRPLKFDEFEALAPQTIFVSATPSEYE
LTKSGGEVAEQVVRPTGLLDPIIEVRPVATQVDDLLSEARIRAANDERILVTTLTKRMAEDLTEYLHEHGVKVRYLHSDI
DTVERVEIIRDLRLGVFDVLVGINLLREGLDMPEVALVAILDADKEGFLRSERSLIQTMGRAARNVNGKAILYADSITKS
MRKAIDETERRREKQLAYNEQRGITPQPLKRSVKDIMELGDIAKSRKQKNSKVVPLAKVAEESAAYQVLTPQQLEKEISK
LEAAMYQHAQNLEFELAAQKRDEIHQLRQQFIANS

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Escherichia coli, GI1786996, Length=677, Percent_Identity=76.9571639586411, Blast_Score=1046, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=309, Percent_Identity=24.2718446601942, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055 [H]

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 76644; Mature: 76513

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAFELVSDYQPAGDQPTAIKQLLEGLEAGLAHQTLLGVTGSGKTFTLANVIATAQRPT
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHEEECCCCCEEEHHHHHHHCCCCE
IILAPNKTLAAQLYGEMKAFFPNNAVEYFVSYYDYYQPEAYVPTTDTFIEKDASVNAHIE
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHH
QMRLSATKALLERKDAVIVASVSAIYGLGDPDSYLKMMLHLRRGDVINQRDMLRRLAELQ
HHHHHHHHHHHHCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
YSRNDMAFERGQFRVRGEVIDIFPAESDQEAVRVEMFDDEIECISLFDPLTGVITSRDLA
HCCCCHHHHCCCEEEECEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCE
RFTIYPKTHYVTPRERILEAIEQIKSELQVRRQYLLDNNKLLEEQRISQRTQFDIEMMNE
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGFCSGIENYSRYLSGRAEGEPPPTLFDYLPHDGLLIIDESHVTVPQIGAMFKGDRSRKE
HHHHCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHH
TLVEFGFRLPSALDNRPLKFDEFEALAPQTIFVSATPSEYELTKSGGEVAEQVVRPTGLL
HHHHHHHHCCHHHCCCCCCCCHHHHCCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCC
DPIIEVRPVATQVDDLLSEARIRAANDERILVTTLTKRMAEDLTEYLHEHGVKVRYLHSD
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCC
IDTVERVEIIRDLRLGVFDVLVGINLLREGLDMPEVALVAILDADKEGFLRSERSLIQTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
GRAARNVNGKAILYADSITKSMRKAIDETERRREKQLAYNEQRGITPQPLKRSVKDIMEL
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GDIAKSRKQKNSKVVPLAKVAEESAAYQVLTPQQLEKEISKLEAAMYQHAQNLEFELAAQ
HHHHHHHHHCCCCCCHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
KRDEIHQLRQQFIANS
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKAFELVSDYQPAGDQPTAIKQLLEGLEAGLAHQTLLGVTGSGKTFTLANVIATAQRPT
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHEEECCCCCEEEHHHHHHHCCCCE
IILAPNKTLAAQLYGEMKAFFPNNAVEYFVSYYDYYQPEAYVPTTDTFIEKDASVNAHIE
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHH
QMRLSATKALLERKDAVIVASVSAIYGLGDPDSYLKMMLHLRRGDVINQRDMLRRLAELQ
HHHHHHHHHHHHCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
YSRNDMAFERGQFRVRGEVIDIFPAESDQEAVRVEMFDDEIECISLFDPLTGVITSRDLA
HCCCCHHHHCCCEEEECEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCE
RFTIYPKTHYVTPRERILEAIEQIKSELQVRRQYLLDNNKLLEEQRISQRTQFDIEMMNE
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGFCSGIENYSRYLSGRAEGEPPPTLFDYLPHDGLLIIDESHVTVPQIGAMFKGDRSRKE
HHHHCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHH
TLVEFGFRLPSALDNRPLKFDEFEALAPQTIFVSATPSEYELTKSGGEVAEQVVRPTGLL
HHHHHHHHCCHHHCCCCCCCCHHHHCCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCC
DPIIEVRPVATQVDDLLSEARIRAANDERILVTTLTKRMAEDLTEYLHEHGVKVRYLHSD
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCC
IDTVERVEIIRDLRLGVFDVLVGINLLREGLDMPEVALVAILDADKEGFLRSERSLIQTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
GRAARNVNGKAILYADSITKSMRKAIDETERRREKQLAYNEQRGITPQPLKRSVKDIMEL
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GDIAKSRKQKNSKVVPLAKVAEESAAYQVLTPQQLEKEISKLEAAMYQHAQNLEFELAAQ
HHHHHHHHHCCCCCCHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
KRDEIHQLRQQFIANS
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]