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The map label for this gene is argT [H]

Identifier: 147673397

GI number: 147673397

Start: 1555801

End: 1556583

Strand: Reverse

Name: argT [H]

Synonym: VC0395_A1454

Alternate gene names: 147673397

Gene position: 1556583-1555801 (Counterclockwise)

Preceding gene: 147674682

Following gene: 147674527

Centisome position: 51.47

GC content: 46.62

Gene sequence:

>783_bases
ATGGATATGAAAAAGTGGTTAGTTGCGACAGCATTAATTGCCACAACAATCAGTGGGGTAACGCAAGCCAAAGAATGGAA
AACGGTGCGTTTTGGTATTGAGGGCGCTTATCCTCCGTTTAGCTGGACAGAAGCCGATGGCTCTTTGAAAGGGTTTGACG
TGGATATGGCCAATGCGTTGTGTGCGGAGATGCAAGTGGAGTGCAAAATCGTGCCACAGGATTGGGATGGCATTATTCCT
TCACTGTTAGCGCGTAAGTACGATGCCATTATTGCAGCGATGTCGATCACTGAAGAGCGTAAAAAGAAAGTGGATTTCAC
TGGTAAGTACGCTCAGATCCCGAACAAGTTTATTGCTAAGAAAGGCGCGGATTTAGCCTTCACTAAAGAAGGTTTGAAGG
GCGTGAAAATTGGTGTGCAGCGTGCGACCACTCACGATAAATACATCACTGACAATTATGGTGACACGGTCGATATCGTG
CGTTATGGCTCGTTTGATGAAGCGTATTTAGACCTTGCCAATGGCCGGATCGCCGCAGTATTAGGGGATGCCTCCGCATT
AGAAGAGGGCATGCTCAACAAACCCGGTGGTGATGCTTATGAATTTGTTGGCCCTTCACTGACAGACCCCAAATGGTTTG
GCGAAGGTATGGGCATAGCGGTGCGCAAGCAAGATAAAGAGCTAACCGAAAAACTCGATGCGGCGATTACGGCACTGCGT
GAAAAAGGGATTTATCAACAAATACAAGCTAAATACTTCAAGTATGACGTTTACGGTGAATAA

Upstream 100 bases:

>100_bases
AGTGCGCAGTCGAACCTGAGTCTCAAAGAGTGAATTTCAAGTAACACATTGATATTAAAAAAATAGTAAACACAACATTA
AGACAAACAATCACAGGAGT

Downstream 100 bases:

>100_bases
AAGCGCTTCTTATCACTTCGAGTTGCCGTCCACAGGACGGTAGCTTGAAATCATGGATTCGCGATTAAAGCTCACAGTAT
TTCGGGTTGTTGGCACTCGT

Product: amino acid ABC transporter, periplasmic amino acid-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDMKKWLVATALIATTISGVTQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANALCAEMQVECKIVPQDWDGIIP
SLLARKYDAIIAAMSITEERKKKVDFTGKYAQIPNKFIAKKGADLAFTKEGLKGVKIGVQRATTHDKYITDNYGDTVDIV
RYGSFDEAYLDLANGRIAAVLGDASALEEGMLNKPGGDAYEFVGPSLTDPKWFGEGMGIAVRKQDKELTEKLDAAITALR
EKGIYQQIQAKYFKYDVYGE

Sequences:

>Translated_260_residues
MDMKKWLVATALIATTISGVTQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANALCAEMQVECKIVPQDWDGIIP
SLLARKYDAIIAAMSITEERKKKVDFTGKYAQIPNKFIAKKGADLAFTKEGLKGVKIGVQRATTHDKYITDNYGDTVDIV
RYGSFDEAYLDLANGRIAAVLGDASALEEGMLNKPGGDAYEFVGPSLTDPKWFGEGMGIAVRKQDKELTEKLDAAITALR
EKGIYQQIQAKYFKYDVYGE
>Mature_260_residues
MDMKKWLVATALIATTISGVTQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANALCAEMQVECKIVPQDWDGIIP
SLLARKYDAIIAAMSITEERKKKVDFTGKYAQIPNKFIAKKGADLAFTKEGLKGVKIGVQRATTHDKYITDNYGDTVDIV
RYGSFDEAYLDLANGRIAAVLGDASALEEGMLNKPGGDAYEFVGPSLTDPKWFGEGMGIAVRKQDKELTEKLDAAITALR
EKGIYQQIQAKYFKYDVYGE

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=39.6153846153846, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=43.6507936507937, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=39.3162393162393, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=41.2698412698413, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1788228, Length=239, Percent_Identity=33.4728033472803, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787031, Length=250, Percent_Identity=29.6, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28712; Mature: 28712

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMKKWLVATALIATTISGVTQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANAL
CCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
CAEMQVECKIVPQDWDGIIPSLLARKYDAIIAAMSITEERKKKVDFTGKYAQIPNKFIAK
HHCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHH
KGADLAFTKEGLKGVKIGVQRATTHDKYITDNYGDTVDIVRYGSFDEAYLDLANGRIAAV
CCCCEEEECCCCCEEEEEEEECCCCCCEEECCCCCEEEEEEECCCCHHHHHHCCCEEEEE
LGDASALEEGMLNKPGGDAYEFVGPSLTDPKWFGEGMGIAVRKQDKELTEKLDAAITALR
ECCHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
EKGIYQQIQAKYFKYDVYGE
HCCHHHHHHHHHHEEEECCC
>Mature Secondary Structure
MDMKKWLVATALIATTISGVTQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANAL
CCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
CAEMQVECKIVPQDWDGIIPSLLARKYDAIIAAMSITEERKKKVDFTGKYAQIPNKFIAK
HHCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHH
KGADLAFTKEGLKGVKIGVQRATTHDKYITDNYGDTVDIVRYGSFDEAYLDLANGRIAAV
CCCCEEEECCCCCEEEEEEEECCCCCCEEECCCCCEEEEEEECCCCHHHHHHCCCEEEEE
LGDASALEEGMLNKPGGDAYEFVGPSLTDPKWFGEGMGIAVRKQDKELTEKLDAAITALR
ECCHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
EKGIYQQIQAKYFKYDVYGE
HCCHHHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]