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The map label for this gene is oppA [H]

Identifier: 147673391

GI number: 147673391

Start: 649416

End: 651047

Strand: Direct

Name: oppA [H]

Synonym: VC0395_A0609

Alternate gene names: 147673391

Gene position: 649416-651047 (Clockwise)

Preceding gene: 147674084

Following gene: 147675147

Centisome position: 21.47

GC content: 46.2

Gene sequence:

>1632_bases
ATGTATAAGAACAAAATCACCCAGGCACTGCTGTTAGGTGCTGGTTTAGCGGTGGCTACCACGTCATTCACCTCTTTTGC
AGCGAATGTTCCTGCTGGTACCAAACTTGCTCCTGTTCAAGAGCTGGTTCGTGGTAACGGTACTGAAGTCGCGTCTCTTG
ATCCGCACAAAACGGAAGGTGTTCCTGAGTCCAACGTGATTCGTGATCTGTTAGAAGGTCTGGTAAACCAAGACGCTAAC
GGTAACGTGGTTCCGGGTGTCGCTGAAAGCTGGGAAACGACAGACAACAAAACCTTTACTTTCCATATTCGTAAAGATGC
GAAATGGTCTAACGGTGACCCTGTTACTGCACACGATTTCGTCTACAGCTTCCAACGTGCTGTCGATCCTGCAACCGCTT
CTCCTTACTCTTGGTATATGGAATACACCAAGATGAAGAATGCGAAAGAAATCATTGCCGGAGAGAAAGACAAATCAACA
CTGGGCGCGAAAGCAGTTGATGATCATACTCTGGTTTTTGAGTTGGAGACTGCCGTTCCTTACTTCGTGATGATGATGGG
TCACACAACGGTGAAACCGATTCACAAAGCGACAGTAGAAAAATTTGGTGATCAGTGGACCAAACCTGAAAACTTTGTCG
GTAACGGTGCTTACGTTCTCGACAAGTGGGTGGTTAACGAGCGTTTAGAGCTCAAGCGTAACCCTCAATACTGGGACGAC
AAAAATACTGTTCTGAATAAAGTGACTTATCTGCCAATCGAAAACCAAGTGGCAGAGATGAACCGTTTCCTATCGGGCGA
AATTCAGATCACGAACGAACTGCCACTTGAGCACTTCAAACGTCTGAAAAAAGAGCATCCAGAATCTGTTCAAGTGCAAG
GCCAACTCTGTACTTATTACTACGGCTTCAATAACAAAAAGCCACCATTTGACGATGTTCGCGTACGTAAAGCCCTTTCT
TACGCGATTGACCGTGATGTGGTTTCTGATGCGATTCTAGGCCAAGGCCAAAAACCGGCTTACTTCCTGACTCCGGAAAT
CACCGCAGGATTTAGCCCAGAAATGCCAGCTTACGGCAAAATGACTCAGAAAGAGCGTATTGCAGAAGCGAAGAAATTGC
TGGAAGAAGCGGGTTACAACAAATCTAACCCTCTGAAATTCACTCTGCTGTACAACACCTCAGAAAACCACAAGAAGATT
GCAACTGCGATCCAATCTATGTGGAAAACTTCTCTGGGCGTTGATATCGCACTGGAAAACCAAGAGTGGAAAACCTACCT
AGATACTCGTCGTCAAGGCAACTTTGATGTGACTCGTGCGGGATGGTGTGGTGACTACAACGAAGCGTCTTCTTTCTTGA
CTCTGATGTTGTCGAGCAACACTTCAAACGATCCGCAGTACCACAGTGCTGAATACGACGCCGTGATTGAAAAAGCGAAA
ACGTCAACTTCTGAAGAAGAGCGTCAAAAACTCTATGCGGATGCAGAGAAGTTGTTGGCTCGTGATATGCCAATCGCGCC
AATCTACCAATACGTAAGATCGCGTCTTGTTTCTCCAACCGTCGGTGGCTACCCATCGAATAACGCGGAAGACAAGATCT
ACGCCAAAGATCTTTACATCAAAGCTCAATAA

Upstream 100 bases:

>100_bases
TGTTTTTGTTTAAAACTTATACGACAAACGACAACAATCAGCCGTTCTGAATACCTGACATGGAAGTCATGGATGCAATA
CTAAAACTTGGAGTTTATGC

Downstream 100 bases:

>100_bases
TAGAGCCATATTCCTAACGAGTTGAAGTTGCAGTGATGACTGCAACTTCATTCGTCGGGGACAAAACTATAAATAACGTA
GACGCTGCATGTGAAATTCA

Product: oligopeptide ABC transporter, periplasmic oligopeptide-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MYKNKITQALLLGAGLAVATTSFTSFAANVPAGTKLAPVQELVRGNGTEVASLDPHKTEGVPESNVIRDLLEGLVNQDAN
GNVVPGVAESWETTDNKTFTFHIRKDAKWSNGDPVTAHDFVYSFQRAVDPATASPYSWYMEYTKMKNAKEIIAGEKDKST
LGAKAVDDHTLVFELETAVPYFVMMMGHTTVKPIHKATVEKFGDQWTKPENFVGNGAYVLDKWVVNERLELKRNPQYWDD
KNTVLNKVTYLPIENQVAEMNRFLSGEIQITNELPLEHFKRLKKEHPESVQVQGQLCTYYYGFNNKKPPFDDVRVRKALS
YAIDRDVVSDAILGQGQKPAYFLTPEITAGFSPEMPAYGKMTQKERIAEAKKLLEEAGYNKSNPLKFTLLYNTSENHKKI
ATAIQSMWKTSLGVDIALENQEWKTYLDTRRQGNFDVTRAGWCGDYNEASSFLTLMLSSNTSNDPQYHSAEYDAVIEKAK
TSTSEEERQKLYADAEKLLARDMPIAPIYQYVRSRLVSPTVGGYPSNNAEDKIYAKDLYIKAQ

Sequences:

>Translated_543_residues
MYKNKITQALLLGAGLAVATTSFTSFAANVPAGTKLAPVQELVRGNGTEVASLDPHKTEGVPESNVIRDLLEGLVNQDAN
GNVVPGVAESWETTDNKTFTFHIRKDAKWSNGDPVTAHDFVYSFQRAVDPATASPYSWYMEYTKMKNAKEIIAGEKDKST
LGAKAVDDHTLVFELETAVPYFVMMMGHTTVKPIHKATVEKFGDQWTKPENFVGNGAYVLDKWVVNERLELKRNPQYWDD
KNTVLNKVTYLPIENQVAEMNRFLSGEIQITNELPLEHFKRLKKEHPESVQVQGQLCTYYYGFNNKKPPFDDVRVRKALS
YAIDRDVVSDAILGQGQKPAYFLTPEITAGFSPEMPAYGKMTQKERIAEAKKLLEEAGYNKSNPLKFTLLYNTSENHKKI
ATAIQSMWKTSLGVDIALENQEWKTYLDTRRQGNFDVTRAGWCGDYNEASSFLTLMLSSNTSNDPQYHSAEYDAVIEKAK
TSTSEEERQKLYADAEKLLARDMPIAPIYQYVRSRLVSPTVGGYPSNNAEDKIYAKDLYIKAQ
>Mature_543_residues
MYKNKITQALLLGAGLAVATTSFTSFAANVPAGTKLAPVQELVRGNGTEVASLDPHKTEGVPESNVIRDLLEGLVNQDAN
GNVVPGVAESWETTDNKTFTFHIRKDAKWSNGDPVTAHDFVYSFQRAVDPATASPYSWYMEYTKMKNAKEIIAGEKDKST
LGAKAVDDHTLVFELETAVPYFVMMMGHTTVKPIHKATVEKFGDQWTKPENFVGNGAYVLDKWVVNERLELKRNPQYWDD
KNTVLNKVTYLPIENQVAEMNRFLSGEIQITNELPLEHFKRLKKEHPESVQVQGQLCTYYYGFNNKKPPFDDVRVRKALS
YAIDRDVVSDAILGQGQKPAYFLTPEITAGFSPEMPAYGKMTQKERIAEAKKLLEEAGYNKSNPLKFTLLYNTSENHKKI
ATAIQSMWKTSLGVDIALENQEWKTYLDTRRQGNFDVTRAGWCGDYNEASSFLTLMLSSNTSNDPQYHSAEYDAVIEKAK
TSTSEEERQKLYADAEKLLARDMPIAPIYQYVRSRLVSPTVGGYPSNNAEDKIYAKDLYIKAQ

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=537, Percent_Identity=52.7001862197393, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI87081878, Length=527, Percent_Identity=51.0436432637571, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1789397, Length=519, Percent_Identity=43.1599229287091, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1787762, Length=527, Percent_Identity=24.6679316888046, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789966, Length=466, Percent_Identity=25.3218884120172, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787052, Length=525, Percent_Identity=23.8095238095238, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789887, Length=503, Percent_Identity=25.6461232604374, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787551, Length=465, Percent_Identity=23.010752688172, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61140; Mature: 61140

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKNKITQALLLGAGLAVATTSFTSFAANVPAGTKLAPVQELVRGNGTEVASLDPHKTEG
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
VPESNVIRDLLEGLVNQDANGNVVPGVAESWETTDNKTFTFHIRKDAKWSNGDPVTAHDF
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHH
VYSFQRAVDPATASPYSWYMEYTKMKNAKEIIAGEKDKSTLGAKAVDDHTLVFELETAVP
HHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCHHCCCCCCCCCEEEEEECHHHH
YFVMMMGHTTVKPIHKATVEKFGDQWTKPENFVGNGAYVLDKWVVNERLELKRNPQYWDD
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCC
KNTVLNKVTYLPIENQVAEMNRFLSGEIQITNELPLEHFKRLKKEHPESVQVQGQLCTYY
CHHHHHHEEEECCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEEEEEEEEE
YGFNNKKPPFDDVRVRKALSYAIDRDVVSDAILGQGQKPAYFLTPEITAGFSPEMPAYGK
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCC
MTQKERIAEAKKLLEEAGYNKSNPLKFTLLYNTSENHKKIATAIQSMWKTSLGVDIALEN
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEC
QEWKTYLDTRRQGNFDVTRAGWCGDYNEASSFLTLMLSSNTSNDPQYHSAEYDAVIEKAK
CHHHHHHHHCCCCCCCEEECCCCCCHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHH
TSTSEEERQKLYADAEKLLARDMPIAPIYQYVRSRLVSPTVGGYPSNNAEDKIYAKDLYI
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEE
KAQ
EEC
>Mature Secondary Structure
MYKNKITQALLLGAGLAVATTSFTSFAANVPAGTKLAPVQELVRGNGTEVASLDPHKTEG
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
VPESNVIRDLLEGLVNQDANGNVVPGVAESWETTDNKTFTFHIRKDAKWSNGDPVTAHDF
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHH
VYSFQRAVDPATASPYSWYMEYTKMKNAKEIIAGEKDKSTLGAKAVDDHTLVFELETAVP
HHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCHHCCCCCCCCCEEEEEECHHHH
YFVMMMGHTTVKPIHKATVEKFGDQWTKPENFVGNGAYVLDKWVVNERLELKRNPQYWDD
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCC
KNTVLNKVTYLPIENQVAEMNRFLSGEIQITNELPLEHFKRLKKEHPESVQVQGQLCTYY
CHHHHHHEEEECCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEEEEEEEEE
YGFNNKKPPFDDVRVRKALSYAIDRDVVSDAILGQGQKPAYFLTPEITAGFSPEMPAYGK
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCC
MTQKERIAEAKKLLEEAGYNKSNPLKFTLLYNTSENHKKIATAIQSMWKTSLGVDIALEN
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEC
QEWKTYLDTRRQGNFDVTRAGWCGDYNEASSFLTLMLSSNTSNDPQYHSAEYDAVIEKAK
CHHHHHHHHCCCCCCCEEECCCCCCHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHH
TSTSEEERQKLYADAEKLLARDMPIAPIYQYVRSRLVSPTVGGYPSNNAEDKIYAKDLYI
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEE
KAQ
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]