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The map label for this gene is menF [H]

Identifier: 147673372

GI number: 147673372

Start: 1680821

End: 1682128

Strand: Reverse

Name: menF [H]

Synonym: VC0395_A1562

Alternate gene names: 147673372

Gene position: 1682128-1680821 (Counterclockwise)

Preceding gene: 147673475

Following gene: 147675176

Centisome position: 55.62

GC content: 52.75

Gene sequence:

>1308_bases
TTGTTGCATTTTTATCAGGCCGTTGCTCAATTGATGGAGCACATCCGTGGCGCGAAGGCTCATGATGTTCGTTTTACTCA
GCCGTTGGCCGATAAGCCTCCCTTTGCGCTGATTGACTGGCTTGATGCCCAACCGATTTTCCCCAAATTTTACTGGCAAT
CGCGTGATACTCGTGAAGAAGTGGTCGCGCTCGGTCAGGTGTACACGTTTACCGATCCTGCTCCGGCGTATGCGATTTTA
GGCGATGAGCAGCGAGTATGGGGTGGACGCTCCTTTGATGGGCATACCGCCAAAAATCCGCGTTGTATGTCTTCCTTCTT
CTTTCTACCACAGATTGAACTGATTCGACGGGATGAACAGTGGTTACTCGCGGTCAATATCAACAATGAGCCGCAGCGCA
CGCTGACCGCACTGCAAAAACTGCTGGTGGAAGTGCCCACTTTGCGTTCGATGACAGCGCACGTCACTCGTGTCGAACAC
ACGCCAACGGCGGCTAACTGGCATGATTTGGTGGAAAAAGTGCTGGCGGGGATCACCGAAAAACAGTTTAAAAAAGTGGT
GTTAGCGCGTAAAACAACGCTGACATTGGATGCACCGCTCAGTGCGGCGCAACTGCTCAAAGCTAGCCATGCCCAAAATC
ACCACAGCTTTCACTTTCTTTTGGCTCTCGATTCTCGCCACAGCTTTATGGGGTCAACCCCAGAGCGGCTCTACCTACGC
CATGGGCAGAATCTGGAAACCGAAGCTTTAGCGGGCACGATTGGTCGGGGAGCTAATGCGACGCAAGATATGGAACTGGC
TCACTGGCTGACGCATGACAGTAAAAACCTCAATGAAAATCAGTTGGTGGTAGAGGACATCATCGAAAGCCTCGCGCCGC
ACGCCGAGCAGATCCATACCCAGCGAGAAGCGCAGCTGGTGCGATTGCGTAAAGTGCAGCATCTGAAAAAACGCATTGAT
GCGCAGCTTAAATCTGGGGTCAATGGCGTGCAGTTACTGGGCGTATTACAACCTACCGCCGCGGTTGCGGGTTTACCTCG
TCAAGCGGCACTGGCTTTCATCGCGGAAAATGAGCCTTTTGCACGCGGCTGGTACGCAGGCTCCATGGGTTACTTTAGCC
ATGCGCAAGCCGAGTTTTGTGTGGCGATCCGCAGTGCTTTAGTGGTGGATAAACAAGTTCAGCTCTATGCAGGCGCTGGC
ATTGTGCCGGGCTCGATTGCTGAGCATGAATGGCAGGAGCTGGATAAAAAGCTCTCTACCTTATTGAGCTTGATAACCGA
CCGCTTACCGCTGGGAGTGGCATCATGA

Upstream 100 bases:

>100_bases
TCACTTGCCGAAGTAAAAGATACCCCATAACCTTGATTTCATAAGGCTCTCTCAATAGAGTAGCCGCAGTTTACCGAATC
TCTCCCCGTACGAGGTCGCT

Downstream 100 bases:

>100_bases
ACCGCGATCAAGCTTTATTGAACCGTCTTTGGTCGCGAGTGTTACTCGAAGAGTTAAGCCGTTTGGGAGTGACACAAGTG
TGTGTGGCTCCGGGCTCTCG

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLHFYQAVAQLMEHIRGAKAHDVRFTQPLADKPPFALIDWLDAQPIFPKFYWQSRDTREEVVALGQVYTFTDPAPAYAIL
GDEQRVWGGRSFDGHTAKNPRCMSSFFFLPQIELIRRDEQWLLAVNINNEPQRTLTALQKLLVEVPTLRSMTAHVTRVEH
TPTAANWHDLVEKVLAGITEKQFKKVVLARKTTLTLDAPLSAAQLLKASHAQNHHSFHFLLALDSRHSFMGSTPERLYLR
HGQNLETEALAGTIGRGANATQDMELAHWLTHDSKNLNENQLVVEDIIESLAPHAEQIHTQREAQLVRLRKVQHLKKRID
AQLKSGVNGVQLLGVLQPTAAVAGLPRQAALAFIAENEPFARGWYAGSMGYFSHAQAEFCVAIRSALVVDKQVQLYAGAG
IVPGSIAEHEWQELDKKLSTLLSLITDRLPLGVAS

Sequences:

>Translated_435_residues
MLHFYQAVAQLMEHIRGAKAHDVRFTQPLADKPPFALIDWLDAQPIFPKFYWQSRDTREEVVALGQVYTFTDPAPAYAIL
GDEQRVWGGRSFDGHTAKNPRCMSSFFFLPQIELIRRDEQWLLAVNINNEPQRTLTALQKLLVEVPTLRSMTAHVTRVEH
TPTAANWHDLVEKVLAGITEKQFKKVVLARKTTLTLDAPLSAAQLLKASHAQNHHSFHFLLALDSRHSFMGSTPERLYLR
HGQNLETEALAGTIGRGANATQDMELAHWLTHDSKNLNENQLVVEDIIESLAPHAEQIHTQREAQLVRLRKVQHLKKRID
AQLKSGVNGVQLLGVLQPTAAVAGLPRQAALAFIAENEPFARGWYAGSMGYFSHAQAEFCVAIRSALVVDKQVQLYAGAG
IVPGSIAEHEWQELDKKLSTLLSLITDRLPLGVAS
>Mature_435_residues
MLHFYQAVAQLMEHIRGAKAHDVRFTQPLADKPPFALIDWLDAQPIFPKFYWQSRDTREEVVALGQVYTFTDPAPAYAIL
GDEQRVWGGRSFDGHTAKNPRCMSSFFFLPQIELIRRDEQWLLAVNINNEPQRTLTALQKLLVEVPTLRSMTAHVTRVEH
TPTAANWHDLVEKVLAGITEKQFKKVVLARKTTLTLDAPLSAAQLLKASHAQNHHSFHFLLALDSRHSFMGSTPERLYLR
HGQNLETEALAGTIGRGANATQDMELAHWLTHDSKNLNENQLVVEDIIESLAPHAEQIHTQREAQLVRLRKVQHLKKRID
AQLKSGVNGVQLLGVLQPTAAVAGLPRQAALAFIAENEPFARGWYAGSMGYFSHAQAEFCVAIRSALVVDKQVQLYAGAG
IVPGSIAEHEWQELDKKLSTLLSLITDRLPLGVAS

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=442, Percent_Identity=39.1402714932127, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1786809, Length=278, Percent_Identity=33.0935251798561, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1787518, Length=316, Percent_Identity=26.8987341772152, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788114, Length=214, Percent_Identity=27.5700934579439, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=208, Percent_Identity=28.8461538461538, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 48671; Mature: 48671

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHFYQAVAQLMEHIRGAKAHDVRFTQPLADKPPFALIDWLDAQPIFPKFYWQSRDTREE
CCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEECCCCCCCCCHHHCCCCCCHHH
VVALGQVYTFTDPAPAYAILGDEQRVWGGRSFDGHTAKNPRCMSSFFFLPQIELIRRDEQ
HHHHHCEEEECCCCCCEEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCE
WLLAVNINNEPQRTLTALQKLLVEVPTLRSMTAHVTRVEHTPTAANWHDLVEKVLAGITE
EEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCH
KQFKKVVLARKTTLTLDAPLSAAQLLKASHAQNHHSFHFLLALDSRHSFMGSTPERLYLR
HHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
HGQNLETEALAGTIGRGANATQDMELAHWLTHDSKNLNENQLVVEDIIESLAPHAEQIHT
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
QREAQLVRLRKVQHLKKRIDAQLKSGVNGVQLLGVLQPTAAVAGLPRQAALAFIAENEPF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHCCCHHHHEEEEECCCCC
ARGWYAGSMGYFSHAQAEFCVAIRSALVVDKQVQLYAGAGIVPGSIAEHEWQELDKKLST
CCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
LLSLITDRLPLGVAS
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MLHFYQAVAQLMEHIRGAKAHDVRFTQPLADKPPFALIDWLDAQPIFPKFYWQSRDTREE
CCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEECCCCCCCCCHHHCCCCCCHHH
VVALGQVYTFTDPAPAYAILGDEQRVWGGRSFDGHTAKNPRCMSSFFFLPQIELIRRDEQ
HHHHHCEEEECCCCCCEEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCE
WLLAVNINNEPQRTLTALQKLLVEVPTLRSMTAHVTRVEHTPTAANWHDLVEKVLAGITE
EEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCH
KQFKKVVLARKTTLTLDAPLSAAQLLKASHAQNHHSFHFLLALDSRHSFMGSTPERLYLR
HHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
HGQNLETEALAGTIGRGANATQDMELAHWLTHDSKNLNENQLVVEDIIESLAPHAEQIHT
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
QREAQLVRLRKVQHLKKRIDAQLKSGVNGVQLLGVLQPTAAVAGLPRQAALAFIAENEPF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHCCCHHHHEEEEECCCCC
ARGWYAGSMGYFSHAQAEFCVAIRSALVVDKQVQLYAGAGIVPGSIAEHEWQELDKKLST
CCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
LLSLITDRLPLGVAS
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]