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The map label for this gene is menD

Identifier: 147675176

GI number: 147675176

Start: 1679112

End: 1680824

Strand: Reverse

Name: menD

Synonym: VC0395_A1561

Alternate gene names: 147675176

Gene position: 1680824-1679112 (Counterclockwise)

Preceding gene: 147673372

Following gene: 147674495

Centisome position: 55.58

GC content: 53.36

Gene sequence:

>1713_bases
ATGAACCGCGATCAAGCTTTATTGAACCGTCTTTGGTCGCGAGTGTTACTCGAAGAGTTAAGCCGTTTGGGAGTGACACA
AGTGTGTGTGGCTCCGGGCTCTCGTTCAACCCCGTTGACGCTTGAGGCTAATGCGAATACGGCTTTCACGTTACATACTC
ACTATGACGAGCGCGGCTTAGGGTTTATGGCGCTTGGTTTAGCGAAAGCTAGCCAACAGCCTGTCGCGGTGATTGTAACC
TCGGGCACTGCAGTGGCGAACTTATTACCAGCCATTGCTGAATCCAAACTCACCGGTGAACGTTTAGTGGTACTCACGGC
GGATCGTCCGCTTGAGCTAGTGGGGTGTGGTGCCAATCAAGCCATTGTGCAATCGGGGATTTTTTCATCTCATGTCACGG
CATCGCTGGAGCTGCCTAGTCCTGCCATCCATCATCCACTTTCTTGGCTACTCACGTCGATTGATGAAGTGATGGCGCGT
CAAGCCCTACTCGGTGGCAGTGTACACATCAATTGCCCATTTCCTGAGCCCTTGTATTCGGCTGGGGATGAGGCGATTTA
TCAACCCTATTTACAACCTGTTCAGCGTTGGCGTGAGCAAGCTCGGCCTTATACCCAAGTTCATCAAGGATTAGTGCAGA
GTGTTCCCGCTGCAATCGATGGCTTGCTGACTAAAGGGGTGGTGATAGTTGGCTCGCTGAGCTTGCAAGAGGCTCAAGCG
GCAAAGCGTTTTGCCAAAGCGATGGGCTGGCCGTTACTGTGCGATCCACAATCGGGCATCAGTAGCCAATGGGCACATTT
TGATCTCTGGCTGCAGCATCCCAAAGCACGTGAACAGCTCAATCAAGCTCAATGTGTGGTGCAATTTGGTTCGCGGATTG
TCTCCAAACGTCTGTTGCAGTGGTTAGAAGCGTGGTGCGCCACGGGTCTTGGCGAGTATCATTACATTGCGCCGCACAGC
GCACGCAATAACCCTTGGCACGCCATGCAGCAGCAGTGGGTGTGTGAGATTAGCCACTGGGTAGACGCTGTGCTCAGCAA
ACGCTTAGCGGGTCAACATACCCAGCAAGGTTGGGCCGATGAGTTAACCCATTATGCGCAGTCGGTTCGCCAACTGGCGC
AACTGCATTTCTCCTCTTCTTCCCTGAGTGAAGTCGCACTGGCGCTAGATTTGACGGAGCGCGCTACGCAAGCGGATCTG
TTTTTGGGCAACAGCCTGATTGTGCGTTTGGTGGATATATTCAGCGCGCTTGATGGGCGTGAAGTGTTCTCCAATCGTGG
CGCATCTGGCATTGATGGCTTAGTCGCCACAGCGTCAGGGGTGCAACGTGCGCGGCAAAAGCCATTACTGATGCTGCTGG
GAGATACCTCGCTACTGTACGATCTCAACTCGCTCGCGTTGATGCGCAATCCTGCGCAGCCGACCGTGATAGTCGTGACC
AATAACGACGGCGGAGCGATTTTCGATCTCCTGCCTGTGCCGAGTGAGCAGCGCGAAGCGCTCTATCAAATGCCGCATGG
AATGGACTTTGCTCACGCCGCATCACAATTTGGTTTAGCGTATTGTGCGGCGCAAACCTTAGAGCACTACCAAACCTTGG
TGGAAGAGCATTTTGCACACGGTGCAGGTACTTTGCTTATCGAGGTGAAAACCCCGCCGCAGCAAGCTTCCATGCACATC
AAACAACTGACATCGCAACTCCATGCTCTCTAA

Upstream 100 bases:

>100_bases
TGCCGGGCTCGATTGCTGAGCATGAATGGCAGGAGCTGGATAAAAAGCTCTCTACCTTATTGAGCTTGATAACCGACCGC
TTACCGCTGGGAGTGGCATC

Downstream 100 bases:

>100_bases
CCAACTGCACTTTGCCAAGCCTACTGCGCGAACACCCTTGGTGGTGTTGGTGCACGGTTTGCTTGGCAGTGGTGCGGATT
GGCAACCGGTTCTCTCTCAT

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MNRDQALLNRLWSRVLLEELSRLGVTQVCVAPGSRSTPLTLEANANTAFTLHTHYDERGLGFMALGLAKASQQPVAVIVT
SGTAVANLLPAIAESKLTGERLVVLTADRPLELVGCGANQAIVQSGIFSSHVTASLELPSPAIHHPLSWLLTSIDEVMAR
QALLGGSVHINCPFPEPLYSAGDEAIYQPYLQPVQRWREQARPYTQVHQGLVQSVPAAIDGLLTKGVVIVGSLSLQEAQA
AKRFAKAMGWPLLCDPQSGISSQWAHFDLWLQHPKAREQLNQAQCVVQFGSRIVSKRLLQWLEAWCATGLGEYHYIAPHS
ARNNPWHAMQQQWVCEISHWVDAVLSKRLAGQHTQQGWADELTHYAQSVRQLAQLHFSSSSLSEVALALDLTERATQADL
FLGNSLIVRLVDIFSALDGREVFSNRGASGIDGLVATASGVQRARQKPLLMLLGDTSLLYDLNSLALMRNPAQPTVIVVT
NNDGGAIFDLLPVPSEQREALYQMPHGMDFAHAASQFGLAYCAAQTLEHYQTLVEEHFAHGAGTLLIEVKTPPQQASMHI
KQLTSQLHAL

Sequences:

>Translated_570_residues
MNRDQALLNRLWSRVLLEELSRLGVTQVCVAPGSRSTPLTLEANANTAFTLHTHYDERGLGFMALGLAKASQQPVAVIVT
SGTAVANLLPAIAESKLTGERLVVLTADRPLELVGCGANQAIVQSGIFSSHVTASLELPSPAIHHPLSWLLTSIDEVMAR
QALLGGSVHINCPFPEPLYSAGDEAIYQPYLQPVQRWREQARPYTQVHQGLVQSVPAAIDGLLTKGVVIVGSLSLQEAQA
AKRFAKAMGWPLLCDPQSGISSQWAHFDLWLQHPKAREQLNQAQCVVQFGSRIVSKRLLQWLEAWCATGLGEYHYIAPHS
ARNNPWHAMQQQWVCEISHWVDAVLSKRLAGQHTQQGWADELTHYAQSVRQLAQLHFSSSSLSEVALALDLTERATQADL
FLGNSLIVRLVDIFSALDGREVFSNRGASGIDGLVATASGVQRARQKPLLMLLGDTSLLYDLNSLALMRNPAQPTVIVVT
NNDGGAIFDLLPVPSEQREALYQMPHGMDFAHAASQFGLAYCAAQTLEHYQTLVEEHFAHGAGTLLIEVKTPPQQASMHI
KQLTSQLHAL
>Mature_570_residues
MNRDQALLNRLWSRVLLEELSRLGVTQVCVAPGSRSTPLTLEANANTAFTLHTHYDERGLGFMALGLAKASQQPVAVIVT
SGTAVANLLPAIAESKLTGERLVVLTADRPLELVGCGANQAIVQSGIFSSHVTASLELPSPAIHHPLSWLLTSIDEVMAR
QALLGGSVHINCPFPEPLYSAGDEAIYQPYLQPVQRWREQARPYTQVHQGLVQSVPAAIDGLLTKGVVIVGSLSLQEAQA
AKRFAKAMGWPLLCDPQSGISSQWAHFDLWLQHPKAREQLNQAQCVVQFGSRIVSKRLLQWLEAWCATGLGEYHYIAPHS
ARNNPWHAMQQQWVCEISHWVDAVLSKRLAGQHTQQGWADELTHYAQSVRQLAQLHFSSSSLSEVALALDLTERATQADL
FLGNSLIVRLVDIFSALDGREVFSNRGASGIDGLVATASGVQRARQKPLLMLLGDTSLLYDLNSLALMRNPAQPTVIVVT
NNDGGAIFDLLPVPSEQREALYQMPHGMDFAHAASQFGLAYCAAQTLEHYQTLVEEHFAHGAGTLLIEVKTPPQQASMHI
KQLTSQLHAL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=568, Percent_Identity=43.8380281690141, Blast_Score=423, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_VIBC3 (A5F6T1)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F6T1
- STRING:   A5F6T1
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1561
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   VCHO345073:VC0395_A1561-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 62296; Mature: 62296

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRDQALLNRLWSRVLLEELSRLGVTQVCVAPGSRSTPLTLEANANTAFTLHTHYDERGL
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCEEEEEEECCCCCCC
GFMALGLAKASQQPVAVIVTSGTAVANLLPAIAESKLTGERLVVLTADRPLELVGCGANQ
CHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCH
AIVQSGIFSSHVTASLELPSPAIHHPLSWLLTSIDEVMARQALLGGSVHINCPFPEPLYS
HHHHHCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHH
AGDEAIYQPYLQPVQRWREQARPYTQVHQGLVQSVPAAIDGLLTKGVVIVGSLSLQEAQA
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHH
AKRFAKAMGWPLLCDPQSGISSQWAHFDLWLQHPKAREQLNQAQCVVQFGSRIVSKRLLQ
HHHHHHHCCCCEEECCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WLEAWCATGLGEYHYIAPHSARNNPWHAMQQQWVCEISHWVDAVLSKRLAGQHTQQGWAD
HHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ELTHYAQSVRQLAQLHFSSSSLSEVALALDLTERATQADLFLGNSLIVRLVDIFSALDGR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
EVFSNRGASGIDGLVATASGVQRARQKPLLMLLGDTSLLYDLNSLALMRNPAQPTVIVVT
HHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEE
NNDGGAIFDLLPVPSEQREALYQMPHGMDFAHAASQFGLAYCAAQTLEHYQTLVEEHFAH
ECCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAGTLLIEVKTPPQQASMHIKQLTSQLHAL
CCCEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRDQALLNRLWSRVLLEELSRLGVTQVCVAPGSRSTPLTLEANANTAFTLHTHYDERGL
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCEEEEEEECCCCCCC
GFMALGLAKASQQPVAVIVTSGTAVANLLPAIAESKLTGERLVVLTADRPLELVGCGANQ
CHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCH
AIVQSGIFSSHVTASLELPSPAIHHPLSWLLTSIDEVMARQALLGGSVHINCPFPEPLYS
HHHHHCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHH
AGDEAIYQPYLQPVQRWREQARPYTQVHQGLVQSVPAAIDGLLTKGVVIVGSLSLQEAQA
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHH
AKRFAKAMGWPLLCDPQSGISSQWAHFDLWLQHPKAREQLNQAQCVVQFGSRIVSKRLLQ
HHHHHHHCCCCEEECCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WLEAWCATGLGEYHYIAPHSARNNPWHAMQQQWVCEISHWVDAVLSKRLAGQHTQQGWAD
HHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ELTHYAQSVRQLAQLHFSSSSLSEVALALDLTERATQADLFLGNSLIVRLVDIFSALDGR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
EVFSNRGASGIDGLVATASGVQRARQKPLLMLLGDTSLLYDLNSLALMRNPAQPTVIVVT
HHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEE
NNDGGAIFDLLPVPSEQREALYQMPHGMDFAHAASQFGLAYCAAQTLEHYQTLVEEHFAH
ECCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAGTLLIEVKTPPQQASMHIKQLTSQLHAL
CCCEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA