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The map label for this gene is ispF

Identifier: 147673364

GI number: 147673364

Start: 51544

End: 52020

Strand: Direct

Name: ispF

Synonym: VC0395_A0057

Alternate gene names: 147673364

Gene position: 51544-52020 (Clockwise)

Preceding gene: 147673316

Following gene: 147673999

Centisome position: 1.7

GC content: 51.78

Gene sequence:

>477_bases
ATGATCAGAATTGGCCACGGCTTTGATGTACACCGTTTTGGCGGCGAAGGTCCCATCATTATTGGTGGAGTGAAGATCCC
TTATGAACAAGGATTGATCGCCCATTCTGACGGTGATGTGGCACTGCACGCATTAAGTGATGCCTTGCTCGGCGCGATTG
CTGCCGGAGACATTGGCCGCCACTTCCCGGATACCGATGATAAATGGAAAGGGGCAGACAGCCGCGAGCTCCTCAAAGAT
GTGTATCGCCGTGTCAAGGCGCAAGGTTATGTGCTTGGTAATGCGGACGTCACTATCATTGCCCAAGCGCCGAAAATGGC
CCCTTATATTCAGGCGATGTGCGCGGCGATTGCCGAAGATCTGGAAACCGATCTGGGCAACATCAATGTCAAAGCGACCA
CGACAGAAAAACTCGGCTTTACCGGACGTAAAGAAGGTATTGCCTGTGAAGCGGTTGTACTTTTGCGCAAAGCTTAA

Upstream 100 bases:

>100_bases
AGCCTGCTTTAGTGGCAGGACGTGCCGATAATCTCAAAATTACTCAACCTGAAGATCTCGCGTTGGCCGAGTTCTATTTA
AGTCGTAATAAGGAAAAATC

Downstream 100 bases:

>100_bases
CTCAGCCTATAAAAAATTTTGGAATTAAGATGACTGATATTCTCTCTCCTCTGGCTTATTTGTGCGGTAAACCGACTGCG
AAGGCCAAACTCAAAGCGCT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MIRIGHGFDVHRFGGEGPIIIGGVKIPYEQGLIAHSDGDVALHALSDALLGAIAAGDIGRHFPDTDDKWKGADSRELLKD
VYRRVKAQGYVLGNADVTIIAQAPKMAPYIQAMCAAIAEDLETDLGNINVKATTTEKLGFTGRKEGIACEAVVLLRKA

Sequences:

>Translated_158_residues
MIRIGHGFDVHRFGGEGPIIIGGVKIPYEQGLIAHSDGDVALHALSDALLGAIAAGDIGRHFPDTDDKWKGADSRELLKD
VYRRVKAQGYVLGNADVTIIAQAPKMAPYIQAMCAAIAEDLETDLGNINVKATTTEKLGFTGRKEGIACEAVVLLRKA
>Mature_158_residues
MIRIGHGFDVHRFGGEGPIIIGGVKIPYEQGLIAHSDGDVALHALSDALLGAIAAGDIGRHFPDTDDKWKGADSRELLKD
VYRRVKAQGYVLGNADVTIIAQAPKMAPYIQAMCAAIAEDLETDLGNINVKATTTEKLGFTGRKEGIACEAVVLLRKA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=75.1592356687898, Blast_Score=215, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_VIBC3 (A5F9D7)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F9D7
- SMR:   A5F9D7
- STRING:   A5F9D7
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0057
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   VCHO345073:VC0395_A0057-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16838; Mature: 16838

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIGHGFDVHRFGGEGPIIIGGVKIPYEQGLIAHSDGDVALHALSDALLGAIAAGDIGR
CEEECCCCEEEEECCCCCEEEEEEECCHHCCEEEECCCCEEHHHHHHHHHHHHHHCCCCC
HFPDTDDKWKGADSRELLKDVYRRVKAQGYVLGNADVTIIAQAPKMAPYIQAMCAAIAED
CCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHH
LETDLGNINVKATTTEKLGFTGRKEGIACEAVVLLRKA
HHCCCCCEEEEEECHHHCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MIRIGHGFDVHRFGGEGPIIIGGVKIPYEQGLIAHSDGDVALHALSDALLGAIAAGDIGR
CEEECCCCEEEEECCCCCEEEEEEECCHHCCEEEECCCCEEHHHHHHHHHHHHHHCCCCC
HFPDTDDKWKGADSRELLKDVYRRVKAQGYVLGNADVTIIAQAPKMAPYIQAMCAAIAED
CCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHH
LETDLGNINVKATTTEKLGFTGRKEGIACEAVVLLRKA
HHCCCCCEEEEEECHHHCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA