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The map label for this gene is ispD [H]

Identifier: 147673316

GI number: 147673316

Start: 50849

End: 51547

Strand: Direct

Name: ispD [H]

Synonym: VC0395_A0056

Alternate gene names: 147673316

Gene position: 50849-51547 (Clockwise)

Preceding gene: 147674770

Following gene: 147673364

Centisome position: 1.68

GC content: 52.5

Gene sequence:

>699_bases
ATGAACAACATGACAGCGATTGTCCCCGCCGCGGGTGTGGGTCGCCGCATGCAAGCGGATCGCCCTAAGCAGTATTTAAC
CTTGCTCGATAAAACGGTGCTCGAACACACCGTTGAACATCTTTTAGAACATCCTCTCATCGAACACGTGGTAGTGGCGG
TGAGTGCTGATGACCCCTATTTTGCGAATTTGCCTCTGGCGCATCATCCGCGTGTGATTCGTGTCGATGGCGGCAAAGAG
CGGGCGGATTCGGTATTGTCGGCACTGGAGTATGTCTGCCAGCATCGCCTCAGTGAATGGGTATTGGTGCATGATGCGGC
GCGTCCGTGTGTGACTCACGCCGATATCACTCAGCTCATCACCACGGCTTTAGCTCATCCGATTGGTGCGATTTTGGCCT
CGCCCGTGCGGGATACCATGAAACGTGGCGATCACTTACAGCAGATTGTGCATACGGTCGATCGCACTGCGCTCTGGCAT
GCGCTGACACCGCAAATGTTTCGTGCCCAATCACTGCGTGAGAGGTTATTCGCCGCTTTACAGCAGCAAGTCACGATTAC
CGATGAAGCCTCAGCCTTTGAATGGTGTGGTGAAAAGCCTGCTTTAGTGGCAGGACGTGCCGATAATCTCAAAATTACTC
AACCTGAAGATCTCGCGTTGGCCGAGTTCTATTTAAGTCGTAATAAGGAAAAATCATGA

Upstream 100 bases:

>100_bases
GCCAAGGGCTAGATGCGATTGAAGAGCGTGCCCGTAACGAATTGGGTATGGTCAAAGATGGAGAAACCTTCTATCGCATC
ATCGGTGAGGAGTCGCGCCA

Downstream 100 bases:

>100_bases
TCAGAATTGGCCACGGCTTTGATGTACACCGTTTTGGCGGCGAAGGTCCCATCATTATTGGTGGAGTGAAGATCCCTTAT
GAACAAGGATTGATCGCCCA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNNMTAIVPAAGVGRRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPYFANLPLAHHPRVIRVDGGKE
RADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLITTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWH
ALTPQMFRAQSLRERLFAALQQQVTITDEASAFEWCGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS

Sequences:

>Translated_232_residues
MNNMTAIVPAAGVGRRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPYFANLPLAHHPRVIRVDGGKE
RADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLITTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWH
ALTPQMFRAQSLRERLFAALQQQVTITDEASAFEWCGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS
>Mature_232_residues
MNNMTAIVPAAGVGRRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPYFANLPLAHHPRVIRVDGGKE
RADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLITTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWH
ALTPQMFRAQSLRERLFAALQQQVTITDEASAFEWCGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=25.8333333333333, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1789104, Length=227, Percent_Identity=55.9471365638767, Blast_Score=253, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25918; Mature: 25918

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01295 ISPD ; PS00485 A_DEAMINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMTAIVPAAGVGRRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPY
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCC
FANLPLAHHPRVIRVDGGKERADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLI
EECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEHCCCCCCHHHHHHHHHHH
TTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWHALTPQMFRAQSLRERLFAAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
QQQVTITDEASAFEWCGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS
HHHCCCCCCCHHHHHCCCCCCEEECCCCCCEECCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNNMTAIVPAAGVGRRMQADRPKQYLTLLDKTVLEHTVEHLLEHPLIEHVVVAVSADDPY
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCC
FANLPLAHHPRVIRVDGGKERADSVLSALEYVCQHRLSEWVLVHDAARPCVTHADITQLI
EECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEHCCCCCCHHHHHHHHHHH
TTALAHPIGAILASPVRDTMKRGDHLQQIVHTVDRTALWHALTPQMFRAQSLRERLFAAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
QQQVTITDEASAFEWCGEKPALVAGRADNLKITQPEDLALAEFYLSRNKEKS
HHHCCCCCCCHHHHHCCCCCCEEECCCCCCEECCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]