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The map label for this gene is argH

Identifier: 147673344

GI number: 147673344

Start: 2366192

End: 2367568

Strand: Reverse

Name: argH

Synonym: VC0395_A2217

Alternate gene names: 147673344

Gene position: 2367568-2366192 (Counterclockwise)

Preceding gene: 147673961

Following gene: 147674528

Centisome position: 78.29

GC content: 50.18

Gene sequence:

>1377_bases
ATGGCATTATGGGGCGGAAGATTTACCCAAGCGGCAGACAGTCGGTTCAAATCATTCAACGATTCCTTACGTTTTGATTA
TCGATTGGCTGAGCAAGATATTGTTGGATCAATCGCTTGGTCAAAAGCCTTGGTCTCCGTCAATGTCTTGAGCGTGCAGG
AGCAACAACAGTTAGAGCAGGCGTTAAATCACCTATTGCAATCGGTTCAGCAAGATCCGGAGCAAATCTTAGCCTCTGAT
GCAGAAGATATTCACTCTTGGGTCGAGCAGAAGCTGATTGAGCAAGTCGGTGATCTTGGTAAAAAACTGCACACAGGGCG
TTCACGTAATGATCAGGTCGCAACCGATCTTAAGCTCTGGTGTCGCGATCAGGGCGTTCATCTGCTGCTTGCTCTGAAAA
CGCTGCAACAACAGTTGGTGGCGGTGGCTGCTGAACATCAATCGACAGTTTTGCCGGGCTACACCCATTTGCAACGCGCG
CAACCAGTAACTTTTACTCATTGGTGTTTAGCTTATTTGGAGATGTTTGAGCGTGATGAGTCCCGTTTGACTGATGCTCT
AGCGCGTTTAAACACCTCACCACTGGGTTCAGGTGCATTAGCGGGAACCGCTTACGCGATTGATCGTGAGGTGTTAGCGG
CGGATCTTGGTTTCACTCGCGCAACACGTAACTCGCTGGATGCGGTCTCCGATCGCGATCATGTGATGGAGCTGATGTCA
GTCGCATCGATCTCTATGCTGCATTTGTCGCGTCTGGCGGAAGATATGATCTTCTACACCACTGGTGAAGCGGGCTTTAT
TGAATTGGCTGATACGGTGACCTCTGGTTCTTCACTGATGCCACAAAAGAAAAACCCCGATGCACTTGAATTGATCCGCG
GCAAAACCGGACGCGTCTATGGTGCATTGGCTGGGATGATGATGACAGTCAAAGCTCTGCCTCTCGCGTACAACAAAGAC
ATGCAAGAAGACAAAGAAGGGCTGTTTGATGCGCTCGACACTTGGTTTGATTGCTTGCAAATGGCGGGACTTTGCTTTGA
TGGCATTAAAGTCAATGCGGCGCGTACGTTAGAAGCGGCCAAGCAAGGCTACTCGAACGCGACTGAATTGGCGGATTATC
TGGTTGCGAAAGGCATTCCATTTCGCGAGGCGCACCATATTGTCGGGGTAGCGGTAGTCGCAGCTATTGGCAAGGGTGTA
GCGTTGGAAGAGTTGTGTCTGGCGGAGCTGCAACAGTTTTCGCCTTTGATTGAGCAGGATGTCTATCCGATCCTGACTAT
TGAGTCTTGTTTAGAGAAACGCTGCGCACTCGGTGGGGTATCGCCCAAACAAGTGGCGCATGCTCTTCAGCAAGCTCAAG
CGCGCGTGAAGTCTTAA

Upstream 100 bases:

>100_bases
CTCACTTGCCGCCTACCTGCCACTCCATGAGTTTGGGGGACATTTTCAGTTATCACATCCTCGACGGCGGTTGCGGTGAG
AGGAAAACAGGAGATTAAGC

Downstream 100 bases:

>100_bases
CATTTCAGCGAGCTGGTCGATCCTATGAGATAAATGGAAAAAAACAGTGGTGCAACTATTTGAGTAATAAGAGGAATCAT
CTCGGAGAAAAAAGATCGTA

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MALWGGRFTQAADSRFKSFNDSLRFDYRLAEQDIVGSIAWSKALVSVNVLSVQEQQQLEQALNHLLQSVQQDPEQILASD
AEDIHSWVEQKLIEQVGDLGKKLHTGRSRNDQVATDLKLWCRDQGVHLLLALKTLQQQLVAVAAEHQSTVLPGYTHLQRA
QPVTFTHWCLAYLEMFERDESRLTDALARLNTSPLGSGALAGTAYAIDREVLAADLGFTRATRNSLDAVSDRDHVMELMS
VASISMLHLSRLAEDMIFYTTGEAGFIELADTVTSGSSLMPQKKNPDALELIRGKTGRVYGALAGMMMTVKALPLAYNKD
MQEDKEGLFDALDTWFDCLQMAGLCFDGIKVNAARTLEAAKQGYSNATELADYLVAKGIPFREAHHIVGVAVVAAIGKGV
ALEELCLAELQQFSPLIEQDVYPILTIESCLEKRCALGGVSPKQVAHALQQAQARVKS

Sequences:

>Translated_458_residues
MALWGGRFTQAADSRFKSFNDSLRFDYRLAEQDIVGSIAWSKALVSVNVLSVQEQQQLEQALNHLLQSVQQDPEQILASD
AEDIHSWVEQKLIEQVGDLGKKLHTGRSRNDQVATDLKLWCRDQGVHLLLALKTLQQQLVAVAAEHQSTVLPGYTHLQRA
QPVTFTHWCLAYLEMFERDESRLTDALARLNTSPLGSGALAGTAYAIDREVLAADLGFTRATRNSLDAVSDRDHVMELMS
VASISMLHLSRLAEDMIFYTTGEAGFIELADTVTSGSSLMPQKKNPDALELIRGKTGRVYGALAGMMMTVKALPLAYNKD
MQEDKEGLFDALDTWFDCLQMAGLCFDGIKVNAARTLEAAKQGYSNATELADYLVAKGIPFREAHHIVGVAVVAAIGKGV
ALEELCLAELQQFSPLIEQDVYPILTIESCLEKRCALGGVSPKQVAHALQQAQARVKS
>Mature_457_residues
ALWGGRFTQAADSRFKSFNDSLRFDYRLAEQDIVGSIAWSKALVSVNVLSVQEQQQLEQALNHLLQSVQQDPEQILASDA
EDIHSWVEQKLIEQVGDLGKKLHTGRSRNDQVATDLKLWCRDQGVHLLLALKTLQQQLVAVAAEHQSTVLPGYTHLQRAQ
PVTFTHWCLAYLEMFERDESRLTDALARLNTSPLGSGALAGTAYAIDREVLAADLGFTRATRNSLDAVSDRDHVMELMSV
ASISMLHLSRLAEDMIFYTTGEAGFIELADTVTSGSSLMPQKKNPDALELIRGKTGRVYGALAGMMMTVKALPLAYNKDM
QEDKEGLFDALDTWFDCLQMAGLCFDGIKVNAARTLEAAKQGYSNATELADYLVAKGIPFREAHHIVGVAVVAAIGKGVA
LEELCLAELQQFSPLIEQDVYPILTIESCLEKRCALGGVSPKQVAHALQQAQARVKS

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=455, Percent_Identity=45.4945054945055, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI68303542, Length=455, Percent_Identity=45.4945054945055, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI68303549, Length=452, Percent_Identity=43.3628318584071, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI68303547, Length=455, Percent_Identity=42.8571428571429, Blast_Score=340, Evalue=2e-93,
Organism=Escherichia coli, GI1790398, Length=456, Percent_Identity=74.780701754386, Blast_Score=705, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321806, Length=458, Percent_Identity=44.1048034934498, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI221473854, Length=448, Percent_Identity=43.3035714285714, Blast_Score=354, Evalue=8e-98,
Organism=Drosophila melanogaster, GI78706858, Length=448, Percent_Identity=43.3035714285714, Blast_Score=354, Evalue=8e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_VIBC3 (A5F4Z3)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4Z3
- SMR:   A5F4Z3
- STRING:   A5F4Z3
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2217
- eggNOG:   COG0165
- HOGENOM:   HBG539632
- OMA:   MAEDLIF
- ProtClustDB:   PRK04833
- BioCyc:   VCHO345073:VC0395_A2217-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 50332; Mature: 50201

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALWGGRFTQAADSRFKSFNDSLRFDYRLAEQDIVGSIAWSKALVSVNVLSVQEQQQLEQ
CCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALNHLLQSVQQDPEQILASDAEDIHSWVEQKLIEQVGDLGKKLHTGRSRNDQVATDLKLW
HHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
CRDQGVHLLLALKTLQQQLVAVAAEHQSTVLPGYTHLQRAQPVTFTHWCLAYLEMFERDE
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
SRLTDALARLNTSPLGSGALAGTAYAIDREVLAADLGFTRATRNSLDAVSDRDHVMELMS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHH
VASISMLHLSRLAEDMIFYTTGEAGFIELADTVTSGSSLMPQKKNPDALELIRGKTGRVY
HHHHHHHHHHHHHHHHEEEECCCCCCEEHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHH
GALAGMMMTVKALPLAYNKDMQEDKEGLFDALDTWFDCLQMAGLCFDGIKVNAARTLEAA
HHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHH
KQGYSNATELADYLVAKGIPFREAHHIVGVAVVAAIGKGVALEELCLAELQQFSPLIEQD
HHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHC
VYPILTIESCLEKRCALGGVSPKQVAHALQQAQARVKS
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA