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The map label for this gene is argG

Identifier: 147673961

GI number: 147673961

Start: 2367801

End: 2369015

Strand: Reverse

Name: argG

Synonym: VC0395_A2218

Alternate gene names: 147673961

Gene position: 2369015-2367801 (Counterclockwise)

Preceding gene: 147675582

Following gene: 147673344

Centisome position: 78.34

GC content: 50.95

Gene sequence:

>1215_bases
ATGAGTAAAGTACAAGTTAAAAAAGTAGTTGTGGCATATTCTGGCGGTCTGGACACGTCGGTGATCATTCCATGGTTAAA
AGAAAATTATGACTGTGAAGTGGTGGCGTTTGTGGCTGACGTTGGTCAAGGTGATGAAGAGCTGAAAGGCGTAGAAGCCA
AAGCACTCTCATCGGGTGCCTCTGAATGTTACATCGTTGACCTGAAAGAAGAATTCGTAAAAGAGTACATCTACCCAACG
CTGAAAACTGGCGCTTACTATGAAGGTAAATACTTACTCGGTACTTCCATGGCGCGTCCGGTGATTGCTAAAGCACAAGT
TGAAATCGCGCGTAAAGTGGGTGCGGATGCACTGGCGCACGGTTGTACTGGCAAAGGTAACGACCAAGTGCGTTTTGAAG
GTGCTTTTGCGGCACTGGCTCCAGATCTGCATGTGATTGCGCCTTGGCGTGAGTGGGATCTGCGTAGCCGTGAAGCGTGT
TTGGATTACCTTGCCGAGCGCAACATTCCCTGTGCCGCGTCATTAACCAAGATCTACTCGCGTGATGCCAACGCATGGCA
CGTTTCTACCGAAGGTGGCGTTCTGGAAAGCACATGGAATGCGCCAAACGAAGATTGCTGGGTGTGGACCGTCGATCCTG
AGCAAGCGCCTAACGAAGCGGAATATGTCACGCTGCAAGTGGCTCATGGCGAAGTGGTCGCTGTTGATGGTGAAGCCATG
ACCCCTTACAACGCTTTGCTGTACCTCAACCAAAAAGGTGCAAAGCATGGTGTAGGTCGTATCGACATCGTGGAAAACCG
TTTGGTGGGTATGAAATCTCGCGGCTGCTACGAAACCCCGGGAGGCACCATCATCATGGAAGCGCTGCGCGCGGTTGAGC
AACTGGTGCTGGATAAAACCTCATTCGAATTCCGTGAAGAGCTGGGTATTAAAGCGTCACACCTTGTTTATGATGGTCGT
TGGTTCACGCCACTACGTCAGGCGGTATTTGCGGCAGCCGATGAGCTAGCGAAAGATGTCAACGGTGAAGTGGTGATTAA
ACTGTATAAAGGCCAAGCCGTTGCAACGCAAAAACGTTCGGCTAACAGCCTCTACTCAGAAGATTTCGCGACCTTTGGTG
CCGATGAAGTGTATGACCACAGCCACGCAGGCGGCTTCATCCGTCTCTATTCGCTGTCAAGCCGCATCCGCGCTCTGAGC
CAAAACAAGCAGTAA

Upstream 100 bases:

>100_bases
CATCGGCACCCGTTTTCTACCTTAAGATTTTAAGTCACTAATTTGATTAAACCTGAGCACTGACCGCCAAGCGCAGAGCC
AGGAATATGGAGATACATCA

Downstream 100 bases:

>100_bases
TCAGCGCAGTCATCGGTTTATATAATAAGGATCGCACTGCGATCCTTATCCCCTTCCTAATTGAAGTTGCAGCGGTGTTG
GCTACGTTCATTCACCCCAA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSKVQVKKVVVAYSGGLDTSVIIPWLKENYDCEVVAFVADVGQGDEELKGVEAKALSSGASECYIVDLKEEFVKEYIYPT
LKTGAYYEGKYLLGTSMARPVIAKAQVEIARKVGADALAHGCTGKGNDQVRFEGAFAALAPDLHVIAPWREWDLRSREAC
LDYLAERNIPCAASLTKIYSRDANAWHVSTEGGVLESTWNAPNEDCWVWTVDPEQAPNEAEYVTLQVAHGEVVAVDGEAM
TPYNALLYLNQKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIIMEALRAVEQLVLDKTSFEFREELGIKASHLVYDGR
WFTPLRQAVFAAADELAKDVNGEVVIKLYKGQAVATQKRSANSLYSEDFATFGADEVYDHSHAGGFIRLYSLSSRIRALS
QNKQ

Sequences:

>Translated_404_residues
MSKVQVKKVVVAYSGGLDTSVIIPWLKENYDCEVVAFVADVGQGDEELKGVEAKALSSGASECYIVDLKEEFVKEYIYPT
LKTGAYYEGKYLLGTSMARPVIAKAQVEIARKVGADALAHGCTGKGNDQVRFEGAFAALAPDLHVIAPWREWDLRSREAC
LDYLAERNIPCAASLTKIYSRDANAWHVSTEGGVLESTWNAPNEDCWVWTVDPEQAPNEAEYVTLQVAHGEVVAVDGEAM
TPYNALLYLNQKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIIMEALRAVEQLVLDKTSFEFREELGIKASHLVYDGR
WFTPLRQAVFAAADELAKDVNGEVVIKLYKGQAVATQKRSANSLYSEDFATFGADEVYDHSHAGGFIRLYSLSSRIRALS
QNKQ
>Mature_403_residues
SKVQVKKVVVAYSGGLDTSVIIPWLKENYDCEVVAFVADVGQGDEELKGVEAKALSSGASECYIVDLKEEFVKEYIYPTL
KTGAYYEGKYLLGTSMARPVIAKAQVEIARKVGADALAHGCTGKGNDQVRFEGAFAALAPDLHVIAPWREWDLRSREACL
DYLAERNIPCAASLTKIYSRDANAWHVSTEGGVLESTWNAPNEDCWVWTVDPEQAPNEAEYVTLQVAHGEVVAVDGEAMT
PYNALLYLNQKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIIMEALRAVEQLVLDKTSFEFREELGIKASHLVYDGRW
FTPLRQAVFAAADELAKDVNGEVVIKLYKGQAVATQKRSANSLYSEDFATFGADEVYDHSHAGGFIRLYSLSSRIRALSQ
NKQ

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=394, Percent_Identity=42.1319796954315, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI16950633, Length=394, Percent_Identity=42.1319796954315, Blast_Score=292, Evalue=4e-79,
Organism=Escherichia coli, GI1789563, Length=363, Percent_Identity=28.9256198347107, Blast_Score=128, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324514, Length=413, Percent_Identity=38.498789346247, Blast_Score=273, Evalue=5e-74,
Organism=Drosophila melanogaster, GI21358151, Length=408, Percent_Identity=40.9313725490196, Blast_Score=280, Evalue=9e-76,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_VIBC3 (A5F4Z4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4Z4
- SMR:   A5F4Z4
- STRING:   A5F4Z4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2218
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   VCHO345073:VC0395_A2218-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44466; Mature: 44334

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 39-39 BINDING 91-91 BINDING 96-96 BINDING 121-121 BINDING 123-123 BINDING 127-127 BINDING 127-127 BINDING 128-128 BINDING 131-131 BINDING 180-180 BINDING 189-189 BINDING 265-265 BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVQVKKVVVAYSGGLDTSVIIPWLKENYDCEVVAFVADVGQGDEELKGVEAKALSSGA
CCCEEEEEEEEEECCCCCCEEEEEHHCCCCCCEEEEEEECCCCCHHHHCCCHHHHHHCCC
SECYIVDLKEEFVKEYIYPTLKTGAYYEGKYLLGTSMARPVIAKAQVEIARKVGADALAH
CCEEEEECHHHHHHHHCCCCCCCCCEECCEEEECCCCCCCHHHHHHHHHHHHHCHHHHHC
GCTGKGNDQVRFEGAFAALAPDLHVIAPWREWDLRSREACLDYLAERNIPCAASLTKIYS
CCCCCCCCEEEEECCHHHHCCCCEEEECCHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHC
RDANAWHVSTEGGVLESTWNAPNEDCWVWTVDPEQAPNEAEYVTLQVAHGEVVAVDGEAM
CCCCEEEEECCCCEEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCEEEEECCCCC
TPYNALLYLNQKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIIMEALRAVEQLVLDKT
CCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
SFEFREELGIKASHLVYDGRWFTPLRQAVFAAADELAKDVNGEVVIKLYKGQAVATQKRS
CHHHHHHHCCEEHEEEECCEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEHHHHC
ANSLYSEDFATFGADEVYDHSHAGGFIRLYSLSSRIRALSQNKQ
CCHHHHHHHHHCCCHHHHCCCCCCCEEEEEEHHHHHHHHHCCCC
>Mature Secondary Structure 
SKVQVKKVVVAYSGGLDTSVIIPWLKENYDCEVVAFVADVGQGDEELKGVEAKALSSGA
CCEEEEEEEEEECCCCCCEEEEEHHCCCCCCEEEEEEECCCCCHHHHCCCHHHHHHCCC
SECYIVDLKEEFVKEYIYPTLKTGAYYEGKYLLGTSMARPVIAKAQVEIARKVGADALAH
CCEEEEECHHHHHHHHCCCCCCCCCEECCEEEECCCCCCCHHHHHHHHHHHHHCHHHHHC
GCTGKGNDQVRFEGAFAALAPDLHVIAPWREWDLRSREACLDYLAERNIPCAASLTKIYS
CCCCCCCCEEEEECCHHHHCCCCEEEECCHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHC
RDANAWHVSTEGGVLESTWNAPNEDCWVWTVDPEQAPNEAEYVTLQVAHGEVVAVDGEAM
CCCCEEEEECCCCEEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCEEEEECCCCC
TPYNALLYLNQKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIIMEALRAVEQLVLDKT
CCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
SFEFREELGIKASHLVYDGRWFTPLRQAVFAAADELAKDVNGEVVIKLYKGQAVATQKRS
CHHHHHHHCCEEHEEEECCEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEHHHHC
ANSLYSEDFATFGADEVYDHSHAGGFIRLYSLSSRIRALSQNKQ
CCHHHHHHHHHCCCHHHHCCCCCCCEEEEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA