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The map label for this gene is mazG [H]

Identifier: 147673315

GI number: 147673315

Start: 2183090

End: 2183926

Strand: Reverse

Name: mazG [H]

Synonym: VC0395_A2028

Alternate gene names: 147673315

Gene position: 2183926-2183090 (Counterclockwise)

Preceding gene: 147673921

Following gene: 147675626

Centisome position: 72.22

GC content: 47.67

Gene sequence:

>837_bases
ATGCCTGATGTGAGGAAAGCCATGTCACACCCGATTGAACAACTCGAACAGATCATGCGCCGCTTGCGTGACCCAGAACA
AGGTTGCCCGTGGGATAAAGAGCAAACCTTCGACACCATTGCACCTTACACCATTGAAGAAACCTTTGAAGTGGTGGATG
CCATCACCCAGCGCGATTGGCCTAACCTGCGTGAAGAGTTGGGGGATTTGCTGTTTCAGGTGATTTTCTACAGCCAAATG
GCCAAAGAACAAGGCTTGTTTGAGTTTCGTGATGTGGTGGAAACGGTCAATGAGAAACTGACCCGCCGCCATCCTCATGT
GTTTGGGGATATCACCTTTGAAAATGACGCGCAAATTCACGCCAATTGGGAAGCGGAAAAAGCCAAAGAAAAAGCGCAAG
CAGGCAAAAGTGAGCAAAGTATTCTAGACTCTGTACCACGTTCGTTGCCCGCTTTATCGCGTGCCCACAAGATCCAGAAA
AAGTGTGCTCAATACGGATTTGATTGGGATAGCCTTGGCCCTGTGGTTGAAAAAGTGCACGAAGAAGTCGAAGAGGTGAT
GGAAGAAGCGCTGCAAGTGGATGTGGATACGCAAAAAGTCGAGGAAGAGCTAGGTGATCTTTTATTCGCAACCGTCAATT
TAGTGCGTCATTTAGGTCATGATCCTGAAATGGCGCTGAGTAAAGCCAACGCCAAATTTGTGCGCCGTTTTCAAGGTGTC
GAGGACAAAGCCAGAGCGAAAAATAAGCCGCTGCACGCGTACACTTTGGCTGAACTCGACGGATTTTGGGATGAAGTAAA
ACTTGAAGAAAAACAGGCAATAAAGTCATTTTCCTGA

Upstream 100 bases:

>100_bases
GTTGGTCAAACGCTTAGGTTAAATCCAACGCAAACAGCGAGGTGTGAAGTGAAAACTTCCACCTCGTTGCTTTTTCAACG
TCAATACGTTTAGGCGCTCA

Downstream 100 bases:

>100_bases
CGGAGTGTGAAAAATAACAATCAATCTACAGTCAAGCTTAATGGTTGATTAAGCAGCAAATTTGTCTTGTGCGAAAAAAG
AGCGCGCTTTGAGACAAAAG

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MPDVRKAMSHPIEQLEQIMRRLRDPEQGCPWDKEQTFDTIAPYTIEETFEVVDAITQRDWPNLREELGDLLFQVIFYSQM
AKEQGLFEFRDVVETVNEKLTRRHPHVFGDITFENDAQIHANWEAEKAKEKAQAGKSEQSILDSVPRSLPALSRAHKIQK
KCAQYGFDWDSLGPVVEKVHEEVEEVMEEALQVDVDTQKVEEELGDLLFATVNLVRHLGHDPEMALSKANAKFVRRFQGV
EDKARAKNKPLHAYTLAELDGFWDEVKLEEKQAIKSFS

Sequences:

>Translated_278_residues
MPDVRKAMSHPIEQLEQIMRRLRDPEQGCPWDKEQTFDTIAPYTIEETFEVVDAITQRDWPNLREELGDLLFQVIFYSQM
AKEQGLFEFRDVVETVNEKLTRRHPHVFGDITFENDAQIHANWEAEKAKEKAQAGKSEQSILDSVPRSLPALSRAHKIQK
KCAQYGFDWDSLGPVVEKVHEEVEEVMEEALQVDVDTQKVEEELGDLLFATVNLVRHLGHDPEMALSKANAKFVRRFQGV
EDKARAKNKPLHAYTLAELDGFWDEVKLEEKQAIKSFS
>Mature_277_residues
PDVRKAMSHPIEQLEQIMRRLRDPEQGCPWDKEQTFDTIAPYTIEETFEVVDAITQRDWPNLREELGDLLFQVIFYSQMA
KEQGLFEFRDVVETVNEKLTRRHPHVFGDITFENDAQIHANWEAEKAKEKAQAGKSEQSILDSVPRSLPALSRAHKIQKK
CAQYGFDWDSLGPVVEKVHEEVEEVMEEALQVDVDTQKVEEELGDLLFATVNLVRHLGHDPEMALSKANAKFVRRFQGVE
DKARAKNKPLHAYTLAELDGFWDEVKLEEKQAIKSFS

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=261, Percent_Identity=58.2375478927203, Blast_Score=315, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 32120; Mature: 31988

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDVRKAMSHPIEQLEQIMRRLRDPEQGCPWDKEQTFDTIAPYTIEETFEVVDAITQRDW
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCC
PNLREELGDLLFQVIFYSQMAKEQGLFEFRDVVETVNEKLTRRHPHVFGDITFENDAQIH
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
ANWEAEKAKEKAQAGKSEQSILDSVPRSLPALSRAHKIQKKCAQYGFDWDSLGPVVEKVH
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EEVEEVMEEALQVDVDTQKVEEELGDLLFATVNLVRHLGHDPEMALSKANAKFVRRFQGV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
EDKARAKNKPLHAYTLAELDGFWDEVKLEEKQAIKSFS
CHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDVRKAMSHPIEQLEQIMRRLRDPEQGCPWDKEQTFDTIAPYTIEETFEVVDAITQRDW
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCC
PNLREELGDLLFQVIFYSQMAKEQGLFEFRDVVETVNEKLTRRHPHVFGDITFENDAQIH
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
ANWEAEKAKEKAQAGKSEQSILDSVPRSLPALSRAHKIQKKCAQYGFDWDSLGPVVEKVH
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EEVEEVMEEALQVDVDTQKVEEELGDLLFATVNLVRHLGHDPEMALSKANAKFVRRFQGV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
EDKARAKNKPLHAYTLAELDGFWDEVKLEEKQAIKSFS
CHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]