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The map label for this gene is hisB

Identifier: 147673310

GI number: 147673310

Start: 729173

End: 730246

Strand: Direct

Name: hisB

Synonym: VC0395_A0705

Alternate gene names: 147673310

Gene position: 729173-730246 (Clockwise)

Preceding gene: 147673356

Following gene: 147675540

Centisome position: 24.11

GC content: 49.81

Gene sequence:

>1074_bases
GTGAGCAAACAACAAAAAATTCTTTTTATCGACCGCGACGGCACCTTAATTGTTGAGCCGCCAGTGGATTTTCAAGTTGA
CCGTTTGGACAAACTCAAACTTGAGCCTTATGTGATCCCAAGCCTGCTCAAGCTACAAGAGGCGGGTTATCGTTTGGTGA
TGGTGACCAATCAGGATGGTTTAGGCACTTCAAGCTACCCGCAAGCGGATTTCGATGCGCCGCATAACATGATGATGGAC
ATTTTTGAATCGCAAGGCGTGAAGTTTAATGATGTGCTGATTTGCCCGCACTTTGAGCGTGATAACTGTTCGTGCCGCAA
ACCGAAACTTGGCTTGGTCAAAGAGTATCTGCAAGGTGGCAAAGTCGATTTCAAATCGTCAGCCGTGATTGGCGATCGCA
TGACCGATCTGCAACTGGCTGAAAATATGGCGATTCGCGGTATTCAATACCACCCACAAACCATGGGGTGGCTGGATATC
GTTAAAGATCTCACCACCAAGCCACGTGTTGCCCAAGTGGTGCGTAAAACCAAAGAGACCGATATTCAGGTTCTAGTCAA
TCTTGACCAAACTGGCGGTAATCAGATTGAAACTGGATTGGGCTTTTTTGATCACATGCTAGATCAAATCGCGACCCACG
GCGGTTTCCAACTGCAACTGAAAGTGGTGGGCGATCTGCACATTGATGATCACCACACGGTGGAAGATACCGCCTTGGCA
CTAGGGCAAGCACTGCGTGAAGCACTGGGTGACAAACGTGGTATTGGACGCTTTGGTTTCACATTGCCCATGGATGAGTG
TTTGGCACAGTGCGCGTTGGATCTCTCGGGCCGCCCGTATCTGAAATTTGATGCGAGCTTTAGCCGCCCGCAAGTGGGCG
ATCTGTCGACTGAGATGGTGTATCACTTCTTTCGCTCATTGACCGATACTTTGGCGTGTACTCTGCACCTCTCTTCCAGT
GGCGATAACGATCACCACATCATTGAGAGCCTGTTTAAAGCGTTTGGCCGCACGCTGCGTCAAGCCATCACGGTGCAAGG
TAACGATCTGCCGAGCAGTAAAGGGGTGCTCTGA

Upstream 100 bases:

>100_bases
CGAAGAGTGTGAAAAAACCGTCGCGTTTATTCGCAACTACTACCAGTAATTTTGTTTACAGCAGCTCGCTGCGCGGGCTG
CTGATGAAAAGGACATTCTT

Downstream 100 bases:

>100_bases
TGACTCAGAATGTGGTGATTATTGATACTGGCTGCGCCAATATCTCCTCGGTCAAATTCGCCATTGAACGATTAGGTTAT
GCGGTGACGATTTCGCGCGA

Product: imidazole glycerol-phosphate dehydratase/histidinol phosphatase

Products: NA

Alternate protein names: Histidinol-phosphatase; Imidazoleglycerol-phosphate dehydratase; IGPD

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKLEPYVIPSLLKLQEAGYRLVMVTNQDGLGTSSYPQADFDAPHNMMMD
IFESQGVKFNDVLICPHFERDNCSCRKPKLGLVKEYLQGGKVDFKSSAVIGDRMTDLQLAENMAIRGIQYHPQTMGWLDI
VKDLTTKPRVAQVVRKTKETDIQVLVNLDQTGGNQIETGLGFFDHMLDQIATHGGFQLQLKVVGDLHIDDHHTVEDTALA
LGQALREALGDKRGIGRFGFTLPMDECLAQCALDLSGRPYLKFDASFSRPQVGDLSTEMVYHFFRSLTDTLACTLHLSSS
GDNDHHIIESLFKAFGRTLRQAITVQGNDLPSSKGVL

Sequences:

>Translated_357_residues
MSKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKLEPYVIPSLLKLQEAGYRLVMVTNQDGLGTSSYPQADFDAPHNMMMD
IFESQGVKFNDVLICPHFERDNCSCRKPKLGLVKEYLQGGKVDFKSSAVIGDRMTDLQLAENMAIRGIQYHPQTMGWLDI
VKDLTTKPRVAQVVRKTKETDIQVLVNLDQTGGNQIETGLGFFDHMLDQIATHGGFQLQLKVVGDLHIDDHHTVEDTALA
LGQALREALGDKRGIGRFGFTLPMDECLAQCALDLSGRPYLKFDASFSRPQVGDLSTEMVYHFFRSLTDTLACTLHLSSS
GDNDHHIIESLFKAFGRTLRQAITVQGNDLPSSKGVL
>Mature_356_residues
SKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKLEPYVIPSLLKLQEAGYRLVMVTNQDGLGTSSYPQADFDAPHNMMMDI
FESQGVKFNDVLICPHFERDNCSCRKPKLGLVKEYLQGGKVDFKSSAVIGDRMTDLQLAENMAIRGIQYHPQTMGWLDIV
KDLTTKPRVAQVVRKTKETDIQVLVNLDQTGGNQIETGLGFFDHMLDQIATHGGFQLQLKVVGDLHIDDHHTVEDTALAL
GQALREALGDKRGIGRFGFTLPMDECLAQCALDLSGRPYLKFDASFSRPQVGDLSTEMVYHFFRSLTDTLACTLHLSSSG
DNDHHIIESLFKAFGRTLRQAITVQGNDLPSSKGVL

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family

Homologues:

Organism=Escherichia coli, GI87082027, Length=353, Percent_Identity=68.5552407932011, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1786399, Length=150, Percent_Identity=30, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324776, Length=214, Percent_Identity=43.4579439252336, Blast_Score=168, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS7_VIBCH (Q9KSX1)

Other databases:

- EMBL:   AE003852
- PIR:   B82238
- RefSeq:   NP_230780.1
- ProteinModelPortal:   Q9KSX1
- GeneID:   2614405
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1135
- TIGR:   VC_1135
- HOGENOM:   HBG289010
- OMA:   QDGLGTP
- ProtClustDB:   PRK05446
- BRENDA:   3.1.3.15
- BRENDA:   4.2.1.19
- GO:   GO:0005737
- HAMAP:   MF_01022
- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR020566
- InterPro:   IPR005954
- InterPro:   IPR006543
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR013954
- InterPro:   IPR020568
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01662
- TIGRFAMs:   TIGR01261
- TIGRFAMs:   TIGR01656

Pfam domain/function: PF00475 IGPD; PF08645 PNK3P; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56784 SSF56784

EC number: =3.1.3.15; =4.2.1.19

Molecular weight: Translated: 39855; Mature: 39724

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00954 IGP_DEHYDRATASE_1; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKLEPYVIPSLLKLQEAGYRLVMVTNQDG
CCCCCEEEEEECCCCEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCC
LGTSSYPQADFDAPHNMMMDIFESQGVKFNDVLICPHFERDNCSCRKPKLGLVKEYLQGG
CCCCCCCCCCCCCCHHHHHHHHHHCCCEECEEEEECCCCCCCCCCCCCCHHHHHHHHCCC
KVDFKSSAVIGDRMTDLQLAENMAIRGIQYHPQTMGWLDIVKDLTTKPRVAQVVRKTKET
CCCCCCCEEECCCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCC
DIQVLVNLDQTGGNQIETGLGFFDHMLDQIATHGGFQLQLKVVGDLHIDDHHTVEDTALA
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHHHHHH
LGQALREALGDKRGIGRFGFTLPMDECLAQCALDLSGRPYLKFDASFSRPQVGDLSTEMV
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHH
YHFFRSLTDTLACTLHLSSSGDNDHHIIESLFKAFGRTLRQAITVQGNDLPSSKGVL
HHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
>Mature Secondary Structure 
SKQQKILFIDRDGTLIVEPPVDFQVDRLDKLKLEPYVIPSLLKLQEAGYRLVMVTNQDG
CCCCEEEEEECCCCEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCC
LGTSSYPQADFDAPHNMMMDIFESQGVKFNDVLICPHFERDNCSCRKPKLGLVKEYLQGG
CCCCCCCCCCCCCCHHHHHHHHHHCCCEECEEEEECCCCCCCCCCCCCCHHHHHHHHCCC
KVDFKSSAVIGDRMTDLQLAENMAIRGIQYHPQTMGWLDIVKDLTTKPRVAQVVRKTKET
CCCCCCCEEECCCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCC
DIQVLVNLDQTGGNQIETGLGFFDHMLDQIATHGGFQLQLKVVGDLHIDDHHTVEDTALA
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHHHHHH
LGQALREALGDKRGIGRFGFTLPMDECLAQCALDLSGRPYLKFDASFSRPQVGDLSTEMV
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHH
YHFFRSLTDTLACTLHLSSSGDNDHHIIESLFKAFGRTLRQAITVQGNDLPSSKGVL
HHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301