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The map label for this gene is dosC [H]

Identifier: 147673258

GI number: 147673258

Start: 2417648

End: 2418586

Strand: Reverse

Name: dosC [H]

Synonym: VC0395_A2269

Alternate gene names: 147673258

Gene position: 2418586-2417648 (Counterclockwise)

Preceding gene: 147674945

Following gene: 147675670

Centisome position: 79.98

GC content: 49.2

Gene sequence:

>939_bases
ATGGTGAATCGTGCCGATTCAGCAAGGTTGTTGTCGGACCAAGATCTACAAGGATATGTCAGCCGCATACTGGATGTGAT
TCCGCTGCCGATCATTATTTCTAAATCTTCTGTGCTCGGACAAGGCGAGCGTGAGCATCTGCATTTTAATAAAGCTTTTG
TGAAAGAGCTCGGGTATACCATGGCGCAGATCCCAAATATCGAGACTTGGTTTGAAAGGGCTTACCCACAAATTGACTAT
CGACAGGAGATCGTTGCGCAATGCCAAAAGACGGTCGATGAAGCGCGGGCGCAAGGCGAAGATGTCGCGCAGATGCCCGC
TTTGATCATGTGTGCTGATGGGGTGCAGCGTTGGTTTTCCGTCTCAGCGCAAATCGAACCGTCCTGTCGTGACGATCTGC
ATATCATTACTTTTCGCAATATTCATGACTATAAAATCTCTCTTGCGGAAGCCGAGCGCCAATCGCAAATTGATCCATTG
ACTCAACTGAAAAATCGCCGAGGTTTCTTCACTTGGAGTGAGCCGATAGCACCTGAGACGCTACTGGGGTTGATAATTTT
TGATATCGACCATTTTAAGCAAATCAACGATACCTACGGTCATCCTGCTGGAGACTATGTGCTGCGCCAAATCGCCGGAT
TGTTGGAGCAAAACTTACCTCCACTGTCTTGCTGTGTGCGTTGGGGCGGTGAAGAGTTTTTGGTCTGCTTTGAATGCGAC
GACATGGCGAATGTGATTGGGCTGGCCGAAAACATACGAGACTATGTTGAGAGCCATACCTTGATGTGGCAAGGACAGCA
GATTGCTTTGACGCTCAGTGCTGGGTGCACCATGGGAGCGATGATGAGTCACAATTGGGACAATTTGTTACACACTGCTG
ATCAAGCGCTGCTGTCGGCGAAACGCTTGGGGCGCAATCGGGTGATTGCTGATCGGTAA

Upstream 100 bases:

>100_bases
GTAACTGGATCACCTTTTAATACAATTTGATGGAAAATTTGCTGAGAGTTTTATCTATAACTATATAAAGAGAGAAAGCG
GTATAAAAAGGAACGGATTT

Downstream 100 bases:

>100_bases
ACAAAAAGTGTTTGTTGGCGTCGGTTTTCTCTCTGGGTGACTTGTCAGCCATCTCGGTTGTGGTTAACGTGACCAAAACC
AAGGAGGCATTGTGTTCCCG

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MVNRADSARLLSDQDLQGYVSRILDVIPLPIIISKSSVLGQGEREHLHFNKAFVKELGYTMAQIPNIETWFERAYPQIDY
RQEIVAQCQKTVDEARAQGEDVAQMPALIMCADGVQRWFSVSAQIEPSCRDDLHIITFRNIHDYKISLAEAERQSQIDPL
TQLKNRRGFFTWSEPIAPETLLGLIIFDIDHFKQINDTYGHPAGDYVLRQIAGLLEQNLPPLSCCVRWGGEEFLVCFECD
DMANVIGLAENIRDYVESHTLMWQGQQIALTLSAGCTMGAMMSHNWDNLLHTADQALLSAKRLGRNRVIADR

Sequences:

>Translated_312_residues
MVNRADSARLLSDQDLQGYVSRILDVIPLPIIISKSSVLGQGEREHLHFNKAFVKELGYTMAQIPNIETWFERAYPQIDY
RQEIVAQCQKTVDEARAQGEDVAQMPALIMCADGVQRWFSVSAQIEPSCRDDLHIITFRNIHDYKISLAEAERQSQIDPL
TQLKNRRGFFTWSEPIAPETLLGLIIFDIDHFKQINDTYGHPAGDYVLRQIAGLLEQNLPPLSCCVRWGGEEFLVCFECD
DMANVIGLAENIRDYVESHTLMWQGQQIALTLSAGCTMGAMMSHNWDNLLHTADQALLSAKRLGRNRVIADR
>Mature_312_residues
MVNRADSARLLSDQDLQGYVSRILDVIPLPIIISKSSVLGQGEREHLHFNKAFVKELGYTMAQIPNIETWFERAYPQIDY
RQEIVAQCQKTVDEARAQGEDVAQMPALIMCADGVQRWFSVSAQIEPSCRDDLHIITFRNIHDYKISLAEAERQSQIDPL
TQLKNRRGFFTWSEPIAPETLLGLIIFDIDHFKQINDTYGHPAGDYVLRQIAGLLEQNLPPLSCCVRWGGEEFLVCFECD
DMANVIGLAENIRDYVESHTLMWQGQQIALTLSAGCTMGAMMSHNWDNLLHTADQALLSAKRLGRNRVIADR

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=38.9937106918239, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787262, Length=214, Percent_Identity=31.7757009345794, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=35.3658536585366, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787802, Length=166, Percent_Identity=32.5301204819277, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081881, Length=204, Percent_Identity=32.843137254902, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=33.5294117647059, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788085, Length=199, Percent_Identity=30.6532663316583, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=26.8156424581006, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=31.8435754189944, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35393; Mature: 35393

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNRADSARLLSDQDLQGYVSRILDVIPLPIIISKSSVLGQGEREHLHFNKAFVKELGYT
CCCCCCCHHHCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCHHHHHHHHHHHHHHCHH
MAQIPNIETWFERAYPQIDYRQEIVAQCQKTVDEARAQGEDVAQMPALIMCADGVQRWFS
HHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHH
VSAQIEPSCRDDLHIITFRNIHDYKISLAEAERQSQIDPLTQLKNRRGFFTWSEPIAPET
CCCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHCCCHHHHHHCCCCCEEECCCCCHHH
LLGLIIFDIDHFKQINDTYGHPAGDYVLRQIAGLLEQNLPPLSCCVRWGGEEFLVCFECD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECC
DMANVIGLAENIRDYVESHTLMWQGQQIALTLSAGCTMGAMMSHNWDNLLHTADQALLSA
CHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHH
KRLGRNRVIADR
HHHCCCCCCCCC
>Mature Secondary Structure
MVNRADSARLLSDQDLQGYVSRILDVIPLPIIISKSSVLGQGEREHLHFNKAFVKELGYT
CCCCCCCHHHCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCHHHHHHHHHHHHHHCHH
MAQIPNIETWFERAYPQIDYRQEIVAQCQKTVDEARAQGEDVAQMPALIMCADGVQRWFS
HHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHH
VSAQIEPSCRDDLHIITFRNIHDYKISLAEAERQSQIDPLTQLKNRRGFFTWSEPIAPET
CCCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHCCCHHHHHHCCCCCEEECCCCCHHH
LLGLIIFDIDHFKQINDTYGHPAGDYVLRQIAGLLEQNLPPLSCCVRWGGEEFLVCFECD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECC
DMANVIGLAENIRDYVESHTLMWQGQQIALTLSAGCTMGAMMSHNWDNLLHTADQALLSA
CHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHH
KRLGRNRVIADR
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]