Click here to switch to the map view.

The map label for this gene is kdsB

Identifier: 147673188

GI number: 147673188

Start: 1573055

End: 1573813

Strand: Reverse

Name: kdsB

Synonym: VC0395_A1466

Alternate gene names: 147673188

Gene position: 1573813-1573055 (Counterclockwise)

Preceding gene: 147673931

Following gene: 147673779

Centisome position: 52.04

GC content: 52.57

Gene sequence:

>759_bases
ATGTCATTTACTGTCGTGATCCCTGCGCGTTACCAATCCACGCGCTTACCGGGTAAGCCTCTCGCCGATATTGGCGGTAA
GCCAATGATTCAGTGGGTATATGAGCAAGCCATGCAAGCGGGAGCCGACCGCGTGATCATTGCAACCGACGATGAGCGTG
TTGAACAAGCGGTTCAAGCTTTCGGTGGTGTGGTGTGTATGACTTCGCCGAATCATCAATCTGGTACAGAACGTCTGGCG
GAAGTGGTGGCGAAAATGGCGATTCCCGCCGATCACATTGTGGTGAATGTGCAGGGTGATGAGCCTTTGATTCCACCGGC
GATTATCCGCCAAGTCGCGGATAACTTAGCGGCATGCAGCGCGCCGATGGCAACGTTGGCGGTGGAAATTGAAGATGAAG
CGGAAGTGTTCAACCCGAATGCGGTGAAAGTGATCACCGATAAAAGCGGTTATGCGCTGTACTTCAGTCGTGCCACGATT
CCTTGGGATCGTGATAACTTCGCTAAAGCGGATAAAGCTATCGTTCAACCTTTGCTGCGCCATATCGGCATCTATGCCTA
CCGCGCAGGCTTTATCAATACCTATCTGGATTGGCAGCCAAGCCAGCTTGAAAAGATTGAATGTTTAGAGCAACTTCGTG
TGTTGTGGCATGGTGAGAAAATCCATGTTGCGGTTGCGTTAGAAGCTCCACCAGCAGGGGTGGACACGCCGGAAGATTTG
GAAGTGGTGCGACGCATCGTGGCTGAGCGTGCGCAGTAG

Upstream 100 bases:

>100_bases
TGATTTGTAAGCTGGATCGTCTGGCTTATCCCATTAAAGAAGGCATTCCGGTGCTTTTAGAGCCGGAAGCTCGCAGCATG
AGCATGGATGAGGGACGCTA

Downstream 100 bases:

>100_bases
TTATCTTTCAGCTAAGCAGAAAAAAGCTCCTTTGCAGGAGCTTTTTTATTAAGTGTTAGGGATGTTGTATTGCAGCCCCA
GTACTTAGATTCGACTTTCC

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSFTVVIPARYQSTRLPGKPLADIGGKPMIQWVYEQAMQAGADRVIIATDDERVEQAVQAFGGVVCMTSPNHQSGTERLA
EVVAKMAIPADHIVVNVQGDEPLIPPAIIRQVADNLAACSAPMATLAVEIEDEAEVFNPNAVKVITDKSGYALYFSRATI
PWDRDNFAKADKAIVQPLLRHIGIYAYRAGFINTYLDWQPSQLEKIECLEQLRVLWHGEKIHVAVALEAPPAGVDTPEDL
EVVRRIVAERAQ

Sequences:

>Translated_252_residues
MSFTVVIPARYQSTRLPGKPLADIGGKPMIQWVYEQAMQAGADRVIIATDDERVEQAVQAFGGVVCMTSPNHQSGTERLA
EVVAKMAIPADHIVVNVQGDEPLIPPAIIRQVADNLAACSAPMATLAVEIEDEAEVFNPNAVKVITDKSGYALYFSRATI
PWDRDNFAKADKAIVQPLLRHIGIYAYRAGFINTYLDWQPSQLEKIECLEQLRVLWHGEKIHVAVALEAPPAGVDTPEDL
EVVRRIVAERAQ
>Mature_251_residues
SFTVVIPARYQSTRLPGKPLADIGGKPMIQWVYEQAMQAGADRVIIATDDERVEQAVQAFGGVVCMTSPNHQSGTERLAE
VVAKMAIPADHIVVNVQGDEPLIPPAIIRQVADNLAACSAPMATLAVEIEDEAEVFNPNAVKVITDKSGYALYFSRATIP
WDRDNFAKADKAIVQPLLRHIGIYAYRAGFINTYLDWQPSQLEKIECLEQLRVLWHGEKIHVAVALEAPPAGVDTPEDLE
VVRRIVAERAQ

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=244, Percent_Identity=68.4426229508197, Blast_Score=339, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_VIBC3 (A5F726)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F726
- SMR:   A5F726
- STRING:   A5F726
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1466
- eggNOG:   COG1212
- HOGENOM:   HBG637773
- OMA:   FSRAPLP
- ProtClustDB:   PRK05450
- BioCyc:   VCHO345073:VC0395_A1466-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27722; Mature: 27591

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTVVIPARYQSTRLPGKPLADIGGKPMIQWVYEQAMQAGADRVIIATDDERVEQAVQA
CCEEEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHH
FGGVVCMTSPNHQSGTERLAEVVAKMAIPADHIVVNVQGDEPLIPPAIIRQVADNLAACS
HCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHC
APMATLAVEIEDEAEVFNPNAVKVITDKSGYALYFSRATIPWDRDNFAKADKAIVQPLLR
CCCEEEEEEECCCCCEECCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
HIGIYAYRAGFINTYLDWQPSQLEKIECLEQLRVLWHGEKIHVAVALEAPPAGVDTPEDL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHH
EVVRRIVAERAQ
HHHHHHHHHCCC
>Mature Secondary Structure 
SFTVVIPARYQSTRLPGKPLADIGGKPMIQWVYEQAMQAGADRVIIATDDERVEQAVQA
CEEEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHH
FGGVVCMTSPNHQSGTERLAEVVAKMAIPADHIVVNVQGDEPLIPPAIIRQVADNLAACS
HCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHC
APMATLAVEIEDEAEVFNPNAVKVITDKSGYALYFSRATIPWDRDNFAKADKAIVQPLLR
CCCEEEEEEECCCCCEECCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
HIGIYAYRAGFINTYLDWQPSQLEKIECLEQLRVLWHGEKIHVAVALEAPPAGVDTPEDL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHH
EVVRRIVAERAQ
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA