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The map label for this gene is rsuA [H]

Identifier: 147673094

GI number: 147673094

Start: 1313445

End: 1314140

Strand: Reverse

Name: rsuA [H]

Synonym: VC0395_A1241

Alternate gene names: 147673094

Gene position: 1314140-1313445 (Counterclockwise)

Preceding gene: 147673935

Following gene: 147674586

Centisome position: 43.46

GC content: 47.7

Gene sequence:

>696_bases
ATGCGTTTAGACAAATTCCTGTGTGATGCGCTCGGTACAACGCGCAAAGAGGCCACTCAACTTCTCAAAAGTGGTGAAGT
AACCGTCGATGATGTGGTGCAAAAAAGCGGTGCATTCAAACTCAAAGAGACGTCGTGCGTTGAGTGGCAAGGCCGTGAAA
TTATGTTGCATGGTCCGCGTTACATCATGATGCACAAACCAGATGGCGTGGTCTGCTCACATGAAGATGGTTTTAACCAA
ACGGCGTTTACCTTGCTTGATGAAGTGAATGTTGAAGATTTGCACTTTGCAGGCCGCTTGGATGTCGATACCACAGGTTT
ACTGCTGATCACTGATGATGGGCAATGGTCGCACCGAGTCACGTCACCAAAACATAAGTGCGAGAAGACCTACCGCGTGT
GGTTGGCGGATCCCATTCAAGCTGATTACGCAGAGCAATTTAACCGAGGTATTGAATTGCGTGGTGAACGTGAGCTGACT
TTGCCTGCGCAACTGGAGGTGATCAGTGAAACGGAAGTGCTATTGACCATCCATGAAGGCAAGTATCACCAAGTGAAGCG
TATGTTTGCCGCTTTGGGTAACAAAGTCATAGGGTTACATCGTGAACGCATTGGTCAGATTACCTTAGATGAAACCCTTG
AACCGGGGGAATACCGCTATTTGACGGAAGAAGAAGTGGCCTCAATCTGGAAATAA

Upstream 100 bases:

>100_bases
GACGGCTTTTGCATCGTTTGATGTCGGCCGCAAGCAATTGCGCTGGGTAATTATTGTGTGAGGTCTGTATAATAGCCGAC
TTCCAGCAGATGAGGTAAGC

Downstream 100 bases:

>100_bases
ACCAAGATTTAGGAAATAGATTGTTTCTGAATTAGCACTAGTTAATCGCGGCGATTATTAAGATCATATACCCAAACAAC
TTGGAGTTGCAGGTAGGCGG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREIMLHGPRYIMMHKPDGVVCSHEDGFNQ
TAFTLLDEVNVEDLHFAGRLDVDTTGLLLITDDGQWSHRVTSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELT
LPAQLEVISETEVLLTIHEGKYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK

Sequences:

>Translated_231_residues
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREIMLHGPRYIMMHKPDGVVCSHEDGFNQ
TAFTLLDEVNVEDLHFAGRLDVDTTGLLLITDDGQWSHRVTSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELT
LPAQLEVISETEVLLTIHEGKYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK
>Mature_231_residues
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREIMLHGPRYIMMHKPDGVVCSHEDGFNQ
TAFTLLDEVNVEDLHFAGRLDVDTTGLLLITDDGQWSHRVTSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELT
LPAQLEVISETEVLLTIHEGKYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=56.9565217391304, Blast_Score=263, Evalue=5e-72,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=25.9414225941423, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081838, Length=183, Percent_Identity=30.6010928961749, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26413; Mature: 26413

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREIMLHGPR
CCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCCEEECCCCEEEECCCEEEEECCE
YIMMHKPDGVVCSHEDGFNQTAFTLLDEVNVEDLHFAGRLDVDTTGLLLITDDGQWSHRV
EEEEECCCCEEECCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEECCCCCCCCC
TSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELTLPAQLEVISETEVLLTIHEG
CCCHHHHHHEEEEEEECCCCHHHHHHHCCCCEECCCCEEECCCEEEEECCCEEEEEEECC
KYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK
CHHHHHHHHHHHCCCEEEEHHHHHCCEEECCCCCCCCEEEECHHHHHHHCC
>Mature Secondary Structure
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREIMLHGPR
CCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCCEEECCCCEEEECCCEEEEECCE
YIMMHKPDGVVCSHEDGFNQTAFTLLDEVNVEDLHFAGRLDVDTTGLLLITDDGQWSHRV
EEEEECCCCEEECCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEECCCCCCCCC
TSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELTLPAQLEVISETEVLLTIHEG
CCCHHHHHHEEEEEEECCCCHHHHHHHCCCCEECCCCEEECCCEEEEECCCEEEEEEECC
KYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK
CHHHHHHHHHHHCCCEEEEHHHHHCCEEECCCCCCCCEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]