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The map label for this gene is ycgF [H]

Identifier: 147673935

GI number: 147673935

Start: 1319350

End: 1320123

Strand: Reverse

Name: ycgF [H]

Synonym: VC0395_A1247

Alternate gene names: 147673935

Gene position: 1320123-1319350 (Counterclockwise)

Preceding gene: 147673091

Following gene: 147673094

Centisome position: 43.65

GC content: 38.5

Gene sequence:

>774_bases
ATGCATATTATTCCTGTGGCGTCAGATCCCAAAACTTGTTCATGTGGTGTGAATGAAAATTCGCTAGATTTTGATTTTAC
TATGGCATTTCAACCTATAGTAAATTGCAGAACGAAAGAAATATTTGGCTATGAAGCTTTAGTGCGAGGTTTAAATAACG
AATCGGCTTATTCGGTTATTTCAAGAGTTAATGAAGATAATCGCTATTTATTTGATCAAATGTGTAGAGTTAAAGCGATT
GCTCTTGCCGCCAAGTTAGGACTGACCAGTAAATTAAGTATAAACTTTCTCCCCAACGCTATTTATGTGCCAGAGCGTTG
TATTCGTACTACGTTAGAGGCCGCGAAACGTTACCAGTTTCCGATCGAAAATATTATGTTCGAATTTACCGAGGCTGAGC
GGGTAGAAGATGTTAATCACATAAAAAGGATTGTCGAATACTATAAATCTTTGGGTTTTCAAACAGCAATTGATGATTTT
GGTTCTGGCTATTCAGGGTTAAATTTATTGGCTGATTTTCAAACCAATATTGTAAAAGTGGATATGGGGTTGATAAGAAA
TATCCATGCCGATCAGGTTCGTCAGTCGATCATGAAAAACTGTTTAAAGCTGTTTAGTGATTTAAATATTCAACCGCTGG
CTGAAGGTGTGGAGAGTCATGCTGAGTTTGCTTGGTTAAAAGCGGCAGGCGTTGAGTTAATGCAAGGCTATTATTTTGCA
AAGCCCGGCTTTGAGAGCCTACCAAGTGTCAATCCTGAGTTCAGCGAAGCGTGA

Upstream 100 bases:

>100_bases
TTTGAGTGACCTTTTTGATGAAGTAAGATTAAGTAGAATCTTTGCTTTATGTGTCGAATATAACTAGTCTTTAATGAGTC
TATAATTAAGGCATAGTCAA

Downstream 100 bases:

>100_bases
ATTGTAAATTAGATTGGTTCTAAAACGGTGAAACCCCGACAGTTGGTAGCTATCGGGGTTTCGAATTTTTAGATTTCTCG
GTTGGTAATGCCGATGATCT

Product: hypothetical protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MHIIPVASDPKTCSCGVNENSLDFDFTMAFQPIVNCRTKEIFGYEALVRGLNNESAYSVISRVNEDNRYLFDQMCRVKAI
ALAAKLGLTSKLSINFLPNAIYVPERCIRTTLEAAKRYQFPIENIMFEFTEAERVEDVNHIKRIVEYYKSLGFQTAIDDF
GSGYSGLNLLADFQTNIVKVDMGLIRNIHADQVRQSIMKNCLKLFSDLNIQPLAEGVESHAEFAWLKAAGVELMQGYYFA
KPGFESLPSVNPEFSEA

Sequences:

>Translated_257_residues
MHIIPVASDPKTCSCGVNENSLDFDFTMAFQPIVNCRTKEIFGYEALVRGLNNESAYSVISRVNEDNRYLFDQMCRVKAI
ALAAKLGLTSKLSINFLPNAIYVPERCIRTTLEAAKRYQFPIENIMFEFTEAERVEDVNHIKRIVEYYKSLGFQTAIDDF
GSGYSGLNLLADFQTNIVKVDMGLIRNIHADQVRQSIMKNCLKLFSDLNIQPLAEGVESHAEFAWLKAAGVELMQGYYFA
KPGFESLPSVNPEFSEA
>Mature_257_residues
MHIIPVASDPKTCSCGVNENSLDFDFTMAFQPIVNCRTKEIFGYEALVRGLNNESAYSVISRVNEDNRYLFDQMCRVKAI
ALAAKLGLTSKLSINFLPNAIYVPERCIRTTLEAAKRYQFPIENIMFEFTEAERVEDVNHIKRIVEYYKSLGFQTAIDDF
GSGYSGLNLLADFQTNIVKVDMGLIRNIHADQVRQSIMKNCLKLFSDLNIQPLAEGVESHAEFAWLKAAGVELMQGYYFA
KPGFESLPSVNPEFSEA

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=245, Percent_Identity=31.4285714285714, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI226510982, Length=233, Percent_Identity=26.6094420600858, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788502, Length=232, Percent_Identity=27.5862068965517, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787541, Length=236, Percent_Identity=30.0847457627119, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790496, Length=224, Percent_Identity=27.6785714285714, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI87081845, Length=227, Percent_Identity=29.9559471365639, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081980, Length=241, Percent_Identity=26.1410788381743, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87081921, Length=236, Percent_Identity=22.4576271186441, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87081743, Length=231, Percent_Identity=24.6753246753247, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082096, Length=231, Percent_Identity=25.5411255411255, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788849, Length=152, Percent_Identity=28.2894736842105, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 29012; Mature: 29012

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIIPVASDPKTCSCGVNENSLDFDFTMAFQPIVNCRTKEIFGYEALVRGLNNESAYSVI
CEEECCCCCCCCEECCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHH
SRVNEDNRYLFDQMCRVKAIALAAKLGLTSKLSINFLPNAIYVPERCIRTTLEAAKRYQF
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHHHHHHHHHHCCC
PIENIMFEFTEAERVEDVNHIKRIVEYYKSLGFQTAIDDFGSGYSGLNLLADFQTNIVKV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHCCEEEE
DMGLIRNIHADQVRQSIMKNCLKLFSDLNIQPLAEGVESHAEFAWLKAAGVELMQGYYFA
CHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KPGFESLPSVNPEFSEA
CCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MHIIPVASDPKTCSCGVNENSLDFDFTMAFQPIVNCRTKEIFGYEALVRGLNNESAYSVI
CEEECCCCCCCCEECCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHH
SRVNEDNRYLFDQMCRVKAIALAAKLGLTSKLSINFLPNAIYVPERCIRTTLEAAKRYQF
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHHHHHHHHHHCCC
PIENIMFEFTEAERVEDVNHIKRIVEYYKSLGFQTAIDDFGSGYSGLNLLADFQTNIVKV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHCCEEEE
DMGLIRNIHADQVRQSIMKNCLKLFSDLNIQPLAEGVESHAEFAWLKAAGVELMQGYYFA
CHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KPGFESLPSVNPEFSEA
CCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]