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The map label for this gene is trpS [H]

Identifier: 161353771

GI number: 161353771

Start: 39440

End: 40492

Strand: Direct

Name: trpS [H]

Synonym: BRADO0033

Alternate gene names: 161353771

Gene position: 39440-40492 (Clockwise)

Preceding gene: 146337206

Following gene: 146337208

Centisome position: 0.53

GC content: 63.25

Gene sequence:

>1053_bases
ATGGCCTTCGTGGAACGGGTTTTCTCCGGCGTTCAGCCGACCGGCAACCTGCATCTCGGCAACTATCTCGGCGCGATCGT
CAACTTCGTGAAGATGCAGGAGACGCACAACTGCATCTATTGCGTCGTCGACATGCACGCGATCACGATGCCGATCGACG
TTTGGGGCGGTCCGGCCGAGCTCGCGCGCAACACCCGCGAGGTCACCGCGGCTTTCATCGCCGCCGGCATCGACCCGAAG
AAGCACATCGTTTTCAACCAGAGCCAGGTCTCCGGCCATGCCGAGCTTGCCTGGATCTTCAATTGCGTGGCGCGCATCGG
CTGGCTGAACCGCATGACGCAGTTCAAGGAGAAGGCCGGCAAGGATCGTGAGAACGCATCGGTCGGCCTGTACGACTATC
CGGTGCTGATGGCGGCCGACATCCTGCTGTATCGCGCGACCCATGTTCCGGTCGGCGAGGACCAGAAGCAGCATCTCGAG
CTCTCGCGCGACATCGCCCAGAAGTTCAACAATGATTTCGGCGACTCGATCCGGGCGCAGGGTTTTGGCGACGGTCTGTT
CTTTCCGCTGCCGGAGCCGTTCATCACCGGTCCCGCGACGCGCGTGATGTCGCTGCGCGACGGCACCAAAAAGATGTCGA
AGTCCGATGCGTCGGACTATTCGCGCATCAACTTGACCGACGACGCCGACACCATCGCGCAGAAGGTCCGCAAGGCCAAA
ACCGACCCGGAACCGCTGCCGAGCGAGGAGAAGGGGCTGGATACGCGTCCCGAGGCCGACAACCTGGTCGGCATCTATGC
GGCGCTGTCGGATCGCAGCAAGGCGGACGTGCTCAGGGAGTTCGGCGGCGGCCAGTTCTCAAGCTTCAAGAACGCGCTGG
TCGAGCTGTGCGTCACCAAGCTCGGTCCGATCGCCGCCGAGATGAAGCGCCTGACGGCCGATCCCGGCTATGTCGATTCC
GTGCTGGTCGACGGCGGCGAACGCGCCCAGGCGATCGCCGCGGAGACGATGAAGACCGCCAAGGACATCGTCGGCTTCAT
CCGCAAGGCCTGA

Upstream 100 bases:

>100_bases
ATCCGGCAGAGATCCGCTCGCGACCTGCCCGCTGGCGACTTGCACGTTTGAGGCGCCCGTGCCAAACGACGCAGCAAATC
CATCATCTGGAACAGCGACC

Downstream 100 bases:

>100_bases
GCTCCCGTAGCCCGGATGAGCGAAGCGACATCCGGGTTCACACGAACTGCATGCTTGAACCCGGATGTCGCTTCGCTCAT
CCGGGCTACGAGACCACCGG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAFVERVFSGVQPTGNLHLGNYLGAIVNFVKMQETHNCIYCVVDMHAITMPIDVWGGPAELARNTREVTAAFIAAGIDPK
KHIVFNQSQVSGHAELAWIFNCVARIGWLNRMTQFKEKAGKDRENASVGLYDYPVLMAADILLYRATHVPVGEDQKQHLE
LSRDIAQKFNNDFGDSIRAQGFGDGLFFPLPEPFITGPATRVMSLRDGTKKMSKSDASDYSRINLTDDADTIAQKVRKAK
TDPEPLPSEEKGLDTRPEADNLVGIYAALSDRSKADVLREFGGGQFSSFKNALVELCVTKLGPIAAEMKRLTADPGYVDS
VLVDGGERAQAIAAETMKTAKDIVGFIRKA

Sequences:

>Translated_350_residues
MAFVERVFSGVQPTGNLHLGNYLGAIVNFVKMQETHNCIYCVVDMHAITMPIDVWGGPAELARNTREVTAAFIAAGIDPK
KHIVFNQSQVSGHAELAWIFNCVARIGWLNRMTQFKEKAGKDRENASVGLYDYPVLMAADILLYRATHVPVGEDQKQHLE
LSRDIAQKFNNDFGDSIRAQGFGDGLFFPLPEPFITGPATRVMSLRDGTKKMSKSDASDYSRINLTDDADTIAQKVRKAK
TDPEPLPSEEKGLDTRPEADNLVGIYAALSDRSKADVLREFGGGQFSSFKNALVELCVTKLGPIAAEMKRLTADPGYVDS
VLVDGGERAQAIAAETMKTAKDIVGFIRKA
>Mature_349_residues
AFVERVFSGVQPTGNLHLGNYLGAIVNFVKMQETHNCIYCVVDMHAITMPIDVWGGPAELARNTREVTAAFIAAGIDPKK
HIVFNQSQVSGHAELAWIFNCVARIGWLNRMTQFKEKAGKDRENASVGLYDYPVLMAADILLYRATHVPVGEDQKQHLEL
SRDIAQKFNNDFGDSIRAQGFGDGLFFPLPEPFITGPATRVMSLRDGTKKMSKSDASDYSRINLTDDADTIAQKVRKAKT
DPEPLPSEEKGLDTRPEADNLVGIYAALSDRSKADVLREFGGGQFSSFKNALVELCVTKLGPIAAEMKRLTADPGYVDSV
LVDGGERAQAIAAETMKTAKDIVGFIRKA

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=346, Percent_Identity=41.907514450867, Blast_Score=277, Evalue=9e-75,
Organism=Homo sapiens, GI41352700, Length=193, Percent_Identity=49.2227979274611, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI1789786, Length=343, Percent_Identity=41.9825072886297, Blast_Score=283, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI71982800, Length=348, Percent_Identity=35.0574712643678, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71982793, Length=348, Percent_Identity=35.0574712643678, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320474, Length=361, Percent_Identity=37.1191135734072, Blast_Score=213, Evalue=5e-56,
Organism=Drosophila melanogaster, GI24666151, Length=343, Percent_Identity=38.4839650145773, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 38372; Mature: 38241

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFVERVFSGVQPTGNLHLGNYLGAIVNFVKMQETHNCIYCVVDMHAITMPIDVWGGPAE
CHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECCCCHHH
LARNTREVTAAFIAAGIDPKKHIVFNQSQVSGHAELAWIFNCVARIGWLNRMTQFKEKAG
HHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDRENASVGLYDYPVLMAADILLYRATHVPVGEDQKQHLELSRDIAQKFNNDFGDSIRAQ
CCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
GFGDGLFFPLPEPFITGPATRVMSLRDGTKKMSKSDASDYSRINLTDDADTIAQKVRKAK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHC
TDPEPLPSEEKGLDTRPEADNLVGIYAALSDRSKADVLREFGGGQFSSFKNALVELCVTK
CCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LGPIAAEMKRLTADPGYVDSVLVDGGERAQAIAAETMKTAKDIVGFIRKA
HCHHHHHHHHHCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFVERVFSGVQPTGNLHLGNYLGAIVNFVKMQETHNCIYCVVDMHAITMPIDVWGGPAE
HHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECCCCHHH
LARNTREVTAAFIAAGIDPKKHIVFNQSQVSGHAELAWIFNCVARIGWLNRMTQFKEKAG
HHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDRENASVGLYDYPVLMAADILLYRATHVPVGEDQKQHLELSRDIAQKFNNDFGDSIRAQ
CCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
GFGDGLFFPLPEPFITGPATRVMSLRDGTKKMSKSDASDYSRINLTDDADTIAQKVRKAK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHC
TDPEPLPSEEKGLDTRPEADNLVGIYAALSDRSKADVLREFGGGQFSSFKNALVELCVTK
CCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LGPIAAEMKRLTADPGYVDSVLVDGGERAQAIAAETMKTAKDIVGFIRKA
HCHHHHHHHHHCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]