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The map label for this gene is otsB [H]

Identifier: 146343707

GI number: 146343707

Start: 7237517

End: 7238344

Strand: Reverse

Name: otsB [H]

Synonym: BRADO6953

Alternate gene names: 146343707

Gene position: 7238344-7237517 (Counterclockwise)

Preceding gene: 146343708

Following gene: 146343706

Centisome position: 97.07

GC content: 65.58

Gene sequence:

>828_bases
TTGAGAATGCCTGAGAATTCCGACCTGATCGTCCCCGGCGCCGCGGAGCCGGCCGAGATCGTGCCGACGCCCCTGAGCCT
CGTGCCGCATCTGCGCGAGGTCGCGATCCTGCTCGACATCGACGGCACCTTGCTCGATCTCGCGCCGACGCCGCGCGAGG
TCTGGGTGCCGCCGGGATTGATGGAGACGCTCAATACTTTGCTGGCACGGACGTCCGGTGCGCTGGCGCTGGTCTCCGGT
CGCTCGCTCAACGACATCGATCTCATCTTCGCGCCCGAGATCTATCCGGCCGTCGGCGGTCACGGCGCGGAGATGCGGCT
GGCCGGCGGCAGCGAGGCCGACGCCATCAAGGCGCCGCCCTTGGACAAGGAGCTGAAGCGCAGGCTGGCGGCGATCGCCA
AGCTCAGCCCGGGCATTCTGCTCGAGGATAAGGGCTATTCGCTGGCGCTGCATTATCGTCTCGCGCCGCAGGCAGAGAAG
GCGATCTACGAAGAGGTGTCGCGCATTCGCGCCGAGCTGCCTGGCGCGCCCATCGAGGTGCTGCCCGGCAAGAGCGTCTG
CGAAATCAAGCATGCCGGCTTCACCAAGGCGAGCGGCGTGCGCGAGCTGATGCGGCGCGCGCCGTTCGAAGGACGTAAGC
CGTTCTTTATCGGCGACGACGTCACCGATGAAACCGTTTTCGCGATCATGCCCGATTTCTCCGGTCTTTCATTCTCGGTC
GGCCGCCGCGCGCTCGGAGTCGACGGGCATTTCGACGCACCCGATGACGTGCGCGAATTTCTTGCACGGCTTGCGACAGG
CGAAGCTAACAGCGTCACGTCACGATGA

Upstream 100 bases:

>100_bases
CCTGATGCTGGTCGCTGCGCTCGTCGCCTGGCGCTATGCGGTGGCGGCGGAGCGCAAATCGCTCGAGCAGGTCGCGCGTC
CGCTCGCCGTGATGGAGTAG

Downstream 100 bases:

>100_bases
GTTCAGCCGGAGCAATATTCGAGTTGTTAGTTCCATTTGCGCGTCGTTCGCAGTTCCGGGTGTCGCGTTTCTTGATTAGA
AATGCTCTGAGTTCACGGAC

Product: trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRMPENSDLIVPGAAEPAEIVPTPLSLVPHLREVAILLDIDGTLLDLAPTPREVWVPPGLMETLNTLLARTSGALALVSG
RSLNDIDLIFAPEIYPAVGGHGAEMRLAGGSEADAIKAPPLDKELKRRLAAIAKLSPGILLEDKGYSLALHYRLAPQAEK
AIYEEVSRIRAELPGAPIEVLPGKSVCEIKHAGFTKASGVRELMRRAPFEGRKPFFIGDDVTDETVFAIMPDFSGLSFSV
GRRALGVDGHFDAPDDVREFLARLATGEANSVTSR

Sequences:

>Translated_275_residues
MRMPENSDLIVPGAAEPAEIVPTPLSLVPHLREVAILLDIDGTLLDLAPTPREVWVPPGLMETLNTLLARTSGALALVSG
RSLNDIDLIFAPEIYPAVGGHGAEMRLAGGSEADAIKAPPLDKELKRRLAAIAKLSPGILLEDKGYSLALHYRLAPQAEK
AIYEEVSRIRAELPGAPIEVLPGKSVCEIKHAGFTKASGVRELMRRAPFEGRKPFFIGDDVTDETVFAIMPDFSGLSFSV
GRRALGVDGHFDAPDDVREFLARLATGEANSVTSR
>Mature_275_residues
MRMPENSDLIVPGAAEPAEIVPTPLSLVPHLREVAILLDIDGTLLDLAPTPREVWVPPGLMETLNTLLARTSGALALVSG
RSLNDIDLIFAPEIYPAVGGHGAEMRLAGGSEADAIKAPPLDKELKRRLAAIAKLSPGILLEDKGYSLALHYRLAPQAEK
AIYEEVSRIRAELPGAPIEVLPGKSVCEIKHAGFTKASGVRELMRRAPFEGRKPFFIGDDVTDETVFAIMPDFSGLSFSV
GRRALGVDGHFDAPDDVREFLARLATGEANSVTSR

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=244, Percent_Identity=36.0655737704918, Blast_Score=147, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 29531; Mature: 29531

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMPENSDLIVPGAAEPAEIVPTPLSLVPHLREVAILLDIDGTLLDLAPTPREVWVPPGL
CCCCCCCCEEECCCCCCHHHCCCCHHHHHHHHHEEEEEECCCCEEECCCCCCEEECCCHH
METLNTLLARTSGALALVSGRSLNDIDLIFAPEIYPAVGGHGAEMRLAGGSEADAIKAPP
HHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHCCCCCCCEEEECCCCCCCCCCCCC
LDKELKRRLAAIAKLSPGILLEDKGYSLALHYRLAPQAEKAIYEEVSRIRAELPGAPIEV
CCHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEE
LPGKSVCEIKHAGFTKASGVRELMRRAPFEGRKPFFIGDDVTDETVFAIMPDFSGLSFSV
CCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEECCCCCCCHHC
GRRALGVDGHFDAPDDVREFLARLATGEANSVTSR
CCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRMPENSDLIVPGAAEPAEIVPTPLSLVPHLREVAILLDIDGTLLDLAPTPREVWVPPGL
CCCCCCCCEEECCCCCCHHHCCCCHHHHHHHHHEEEEEECCCCEEECCCCCCEEECCCHH
METLNTLLARTSGALALVSGRSLNDIDLIFAPEIYPAVGGHGAEMRLAGGSEADAIKAPP
HHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHCCCCCCCEEEECCCCCCCCCCCCC
LDKELKRRLAAIAKLSPGILLEDKGYSLALHYRLAPQAEKAIYEEVSRIRAELPGAPIEV
CCHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEE
LPGKSVCEIKHAGFTKASGVRELMRRAPFEGRKPFFIGDDVTDETVFAIMPDFSGLSFSV
CCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEECCCCCCCHHC
GRRALGVDGHFDAPDDVREFLARLATGEANSVTSR
CCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]