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The map label for this gene is yafC [H]

Identifier: 146343678

GI number: 146343678

Start: 7195437

End: 7196375

Strand: Reverse

Name: yafC [H]

Synonym: BRADO6921

Alternate gene names: 146343678

Gene position: 7196375-7195437 (Counterclockwise)

Preceding gene: 146343680

Following gene: 146343677

Centisome position: 96.51

GC content: 62.09

Gene sequence:

>939_bases
ATGATTCAGCTCACCGACGTGCGCGTTTTTCTGGAAGTCGTGAGCGCCGGAAGCTTCACGACGGCGGCTCGGACTCTGAA
AATTCCGAAGTCGAGCGTGGCGCGCCAGATCGCGCGCCTGGAGGACGACATTGGCGTCATGCTGCTGGCGAGAACCACAC
GAACGCTCGAATTGACCGACCATGGCCGAACCTTCCTGCCATATGCGAGGCGGTTGCTCGACGATGGCATCGAGGCGAGC
AACGTCATTCGCAGTCATCGGGACGGGGCAAGCGGGCTGCTCACCGTGACAGCGTCGAGCACGTTCGGCCGCATGTTCCT
CGCGCCGCACCTGTCCACGTTTCGCAAGCAGCATCCGAACATTCGCGTTGATCTGAAGCTGACGGCAACGAAACTGGTGA
TTGGTGTGGGCCAGGCGGATATCGCGATCCGGCTGGGCCCGATCGTCGATCCCAATCTCGGCGTGCAGAGCTTCGGCAGA
ATGGAATTCTGCCTCGTTGCGGCGCCGGCCTACATGAAAGGCCGCCCCTCCGTGACGAAGCCGATGGACCTCGCCAGACA
CGAGCTGATCGAGCTGCGGCCGCCGGCCAGCGACAATCAGCTCGAGCTCCACAAGGACGGCAAACTGCAATGCGTCCGCT
GCGTGCCGGCCATGGTCATCGACGATCCCGACACGGCAAAGGTCGTTTGCCTGACGGCCGGCGGGATCGCGACCGTGCCG
ACATTTCTGGTGGCCAATGAAATCCGAGATGCAACCCTGGTGAGGATACTGCCGGACTGGACGCTGGCTCCCGCTCCGAT
CAGCGTGGTCTATGCAAAGCAGATCGCTCCAAGCCTCCGCGTCCGCGCCTATCTCGAGTTTCTCGCGTCGACGATCGGAC
AAACACGACCATGGGAAGTCGAGTTGACGCCGCCGAAAGTCAAGCGAAGCTCGAGCTGA

Upstream 100 bases:

>100_bases
AAAGAATTCGGTTTCTCTCCGCGACGGGACGAATGGTAGGCTGGCGCCCGTTCTGATACAATACGGCAAAACTGAACTTA
TTGACCCGAATACGCAAACA

Downstream 100 bases:

>100_bases
ACCCCGGCGAAACGGCGTCCAGTTGCCAATGTAAGCGCGTGATGGGCCACGTTTGGCGAGGATGTTCGCCTGGCCTTGCA
GGTTCGCCGGCAGCTTGGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIQLTDVRVFLEVVSAGSFTTAARTLKIPKSSVARQIARLEDDIGVMLLARTTRTLELTDHGRTFLPYARRLLDDGIEAS
NVIRSHRDGASGLLTVTASSTFGRMFLAPHLSTFRKQHPNIRVDLKLTATKLVIGVGQADIAIRLGPIVDPNLGVQSFGR
MEFCLVAAPAYMKGRPSVTKPMDLARHELIELRPPASDNQLELHKDGKLQCVRCVPAMVIDDPDTAKVVCLTAGGIATVP
TFLVANEIRDATLVRILPDWTLAPAPISVVYAKQIAPSLRVRAYLEFLASTIGQTRPWEVELTPPKVKRSSS

Sequences:

>Translated_312_residues
MIQLTDVRVFLEVVSAGSFTTAARTLKIPKSSVARQIARLEDDIGVMLLARTTRTLELTDHGRTFLPYARRLLDDGIEAS
NVIRSHRDGASGLLTVTASSTFGRMFLAPHLSTFRKQHPNIRVDLKLTATKLVIGVGQADIAIRLGPIVDPNLGVQSFGR
MEFCLVAAPAYMKGRPSVTKPMDLARHELIELRPPASDNQLELHKDGKLQCVRCVPAMVIDDPDTAKVVCLTAGGIATVP
TFLVANEIRDATLVRILPDWTLAPAPISVVYAKQIAPSLRVRAYLEFLASTIGQTRPWEVELTPPKVKRSSS
>Mature_312_residues
MIQLTDVRVFLEVVSAGSFTTAARTLKIPKSSVARQIARLEDDIGVMLLARTTRTLELTDHGRTFLPYARRLLDDGIEAS
NVIRSHRDGASGLLTVTASSTFGRMFLAPHLSTFRKQHPNIRVDLKLTATKLVIGVGQADIAIRLGPIVDPNLGVQSFGR
MEFCLVAAPAYMKGRPSVTKPMDLARHELIELRPPASDNQLELHKDGKLQCVRCVPAMVIDDPDTAKVVCLTAGGIATVP
TFLVANEIRDATLVRILPDWTLAPAPISVVYAKQIAPSLRVRAYLEFLASTIGQTRPWEVELTPPKVKRSSS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=30.5841924398625, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=32.8813559322034, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=26.0135135135135, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=27.5167785234899, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=23.7931034482759, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=24.496644295302, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787589, Length=262, Percent_Identity=26.3358778625954, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789173, Length=188, Percent_Identity=29.7872340425532, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=150, Percent_Identity=30.6666666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790208, Length=191, Percent_Identity=31.9371727748691, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=202, Percent_Identity=24.7524752475248, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788481, Length=181, Percent_Identity=28.1767955801105, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787601, Length=176, Percent_Identity=23.2954545454545, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790011, Length=323, Percent_Identity=21.671826625387, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34135; Mature: 34135

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQLTDVRVFLEVVSAGSFTTAARTLKIPKSSVARQIARLEDDIGVMLLARTTRTLELTD
CEEEHHHHHHHHHHHCCCCHHHHHEEECCHHHHHHHHHHHCCCCCEEEEEECCEEEEECC
HGRTFLPYARRLLDDGIEASNVIRSHRDGASGLLTVTASSTFGRMFLAPHLSTFRKQHPN
CCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCC
IRVDLKLTATKLVIGVGQADIAIRLGPIVDPNLGVQSFGRMEFCLVAAPAYMKGRPSVTK
EEEEEEEEEEEEEEECCCCCEEEEECCCCCCCCCHHHCCCEEEEEEECCHHHCCCCCCCC
PMDLARHELIELRPPASDNQLELHKDGKLQCVRCVPAMVIDDPDTAKVVCLTAGGIATVP
CHHHHHHHHEEECCCCCCCEEEEECCCCEEEEEECCEEEECCCCCCEEEEEECCCHHHHH
TFLVANEIRDATLVRILPDWTLAPAPISVVYAKQIAPSLRVRAYLEFLASTIGQTRPWEV
HHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
ELTPPKVKRSSS
EECCCCCCCCCC
>Mature Secondary Structure
MIQLTDVRVFLEVVSAGSFTTAARTLKIPKSSVARQIARLEDDIGVMLLARTTRTLELTD
CEEEHHHHHHHHHHHCCCCHHHHHEEECCHHHHHHHHHHHCCCCCEEEEEECCEEEEECC
HGRTFLPYARRLLDDGIEASNVIRSHRDGASGLLTVTASSTFGRMFLAPHLSTFRKQHPN
CCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCC
IRVDLKLTATKLVIGVGQADIAIRLGPIVDPNLGVQSFGRMEFCLVAAPAYMKGRPSVTK
EEEEEEEEEEEEEEECCCCCEEEEECCCCCCCCCHHHCCCEEEEEEECCHHHCCCCCCCC
PMDLARHELIELRPPASDNQLELHKDGKLQCVRCVPAMVIDDPDTAKVVCLTAGGIATVP
CHHHHHHHHEEECCCCCCCEEEEECCCCEEEEEECCEEEECCCCCCEEEEEECCCHHHHH
TFLVANEIRDATLVRILPDWTLAPAPISVVYAKQIAPSLRVRAYLEFLASTIGQTRPWEV
HHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
ELTPPKVKRSSS
EECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]