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The map label for this gene is prfH [H]

Identifier: 146343293

GI number: 146343293

Start: 6764818

End: 6765441

Strand: Direct

Name: prfH [H]

Synonym: BRADO6515

Alternate gene names: 146343293

Gene position: 6764818-6765441 (Clockwise)

Preceding gene: 146343292

Following gene: 146343294

Centisome position: 90.72

GC content: 73.72

Gene sequence:

>624_bases
ATGAGACGGCTGCTCCTCACCTCGGGACGCGGTCCGGCGGAATGCCGGATCGCGGTCGCACATGCGCTCGCGCGTCTCGC
GGACGAAGCCGCGGCGGCGGACTGCGACTGCGCGATCGCGCCAAGCGACGCCGACCGCCACGGACCGGCCAGCGCGATCG
CCGTGCTGGACGGAACGGGCGCCGACGCGATCGCGCAACGCTGGACCCGGGGCTCGCTGCTCTGGGTGTGCCGCAGTCCG
TTGCGGCCACATCACGGCCGCAAGAACTGGTTCGTCGGGATCGTCGATCTGCCGCTGCCCGCGCAGCTGCCGATGCTGTC
GACCAGTGATGTCCGCTTCGAGAGCTTCCGCGCCGGCGGCCCCGGCGGCCAGCACCAGAACAAGACCGAGAGCGCGGTGC
GCGCCGTCCACCTGCCGAGCGGCCTCACCGCGATCGCCCGCGACGGCCGCTCGCAGCATGGCAACAAGGCTCTGGCGATC
GCCCGCCTCGCCGCCCTGCTCGATGGCCGTGCGCGCGTCGCCGAAGCCGGCGAGGTCCGGCTGGTGCAGGCCGCGCATGG
CCGCGTCGAACGCGGCGCCGCCGGCCTGCGCTTCAAGGGACTGACGTTCAGGCCGGCGGGGTGA

Upstream 100 bases:

>100_bases
ATCTTGAGGCGTTCGGGCTCGCGCGCGTCGTCGCCAGCTTCCGGCCGCTGGTGACGGTGAAGAAGATCGCCACCGAGCGG
CGGCCGAAGGAGGAGCGCCG

Downstream 100 bases:

>100_bases
TCAACGAGAGGCTGGGGAGTGAATCTCCCTGGCCGCCACATCGACGATCGGACTTTGCGTCCGCCTCTCTCCACGCCGTC
ATGGCCCGGCTTGTCCCGGC

Product: class I peptide chain release factor domain-containing protein

Products: NA

Alternate protein names: RF-H [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MRRLLLTSGRGPAECRIAVAHALARLADEAAAADCDCAIAPSDADRHGPASAIAVLDGTGADAIAQRWTRGSLLWVCRSP
LRPHHGRKNWFVGIVDLPLPAQLPMLSTSDVRFESFRAGGPGGQHQNKTESAVRAVHLPSGLTAIARDGRSQHGNKALAI
ARLAALLDGRARVAEAGEVRLVQAAHGRVERGAAGLRFKGLTFRPAG

Sequences:

>Translated_207_residues
MRRLLLTSGRGPAECRIAVAHALARLADEAAAADCDCAIAPSDADRHGPASAIAVLDGTGADAIAQRWTRGSLLWVCRSP
LRPHHGRKNWFVGIVDLPLPAQLPMLSTSDVRFESFRAGGPGGQHQNKTESAVRAVHLPSGLTAIARDGRSQHGNKALAI
ARLAALLDGRARVAEAGEVRLVQAAHGRVERGAAGLRFKGLTFRPAG
>Mature_207_residues
MRRLLLTSGRGPAECRIAVAHALARLADEAAAADCDCAIAPSDADRHGPASAIAVLDGTGADAIAQRWTRGSLLWVCRSP
LRPHHGRKNWFVGIVDLPLPAQLPMLSTSDVRFESFRAGGPGGQHQNKTESAVRAVHLPSGLTAIARDGRSQHGNKALAI
ARLAALLDGRARVAEAGEVRLVQAAHGRVERGAAGLRFKGLTFRPAG

Specific function: Could be involved in translational termination [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=77, Percent_Identity=45.4545454545455, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI166795303, Length=110, Percent_Identity=37.2727272727273, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787462, Length=70, Percent_Identity=48.5714285714286, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI2367172, Length=62, Percent_Identity=43.5483870967742, Blast_Score=64, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321295, Length=58, Percent_Identity=51.7241379310345, Blast_Score=62, Evalue=8e-11,
Organism=Drosophila melanogaster, GI19921226, Length=139, Percent_Identity=36.6906474820144, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352
- InterPro:   IPR017509 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 21788; Mature: 21788

Theoretical pI: Translated: 11.41; Mature: 11.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLLLTSGRGPAECRIAVAHALARLADEAAAADCDCAIAPSDADRHGPASAIAVLDGTG
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEEECCC
ADAIAQRWTRGSLLWVCRSPLRPHHGRKNWFVGIVDLPLPAQLPMLSTSDVRFESFRAGG
HHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHEECCC
PGGQHQNKTESAVRAVHLPSGLTAIARDGRSQHGNKALAIARLAALLDGRARVAEAGEVR
CCCCCCCHHHHHHEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCEE
LVQAAHGRVERGAAGLRFKGLTFRPAG
EEEHHCCCHHCCCCCEEECCEEECCCC
>Mature Secondary Structure
MRRLLLTSGRGPAECRIAVAHALARLADEAAAADCDCAIAPSDADRHGPASAIAVLDGTG
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEEECCC
ADAIAQRWTRGSLLWVCRSPLRPHHGRKNWFVGIVDLPLPAQLPMLSTSDVRFESFRAGG
HHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHEECCC
PGGQHQNKTESAVRAVHLPSGLTAIARDGRSQHGNKALAIARLAALLDGRARVAEAGEVR
CCCCCCCHHHHHHEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCEE
LVQAAHGRVERGAAGLRFKGLTFRPAG
EEEHHCCCHHCCCCCEEECCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1695895; 9278503; 1408743 [H]