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The map label for this gene is spo0F [H]

Identifier: 146343252

GI number: 146343252

Start: 6724731

End: 6725111

Strand: Direct

Name: spo0F [H]

Synonym: BRADO6472

Alternate gene names: 146343252

Gene position: 6724731-6725111 (Clockwise)

Preceding gene: 146343251

Following gene: 146343253

Centisome position: 90.19

GC content: 65.88

Gene sequence:

>381_bases
ATGTCGCGCGTCCTGATCGCCGACGACGAAGATTCGATGCGCACCCTGGTCGCGCGCGCGATCGCGATGGACGGCCACGA
GACCGTCACCGCGCAGGATGGCGCCGAGGCGCTCGACATCCTCACGCGTGAGAACGGCGCGTTCGATCTGCTGCTGACCG
ACATCCAGATGCCCATCATGGACGGCATCGCGCTGGCGCTGTCGGCCGCGCGTGATTTTCCCGACCTGACCATCCTTCTG
ATGACCGGCTTCGCCCATCAGCGCGAGCGGGCGTCGAATCTGAATGCGATCGCTCATGACGTGATCACCAAGCCGTTCTC
GGTCGCCGACATCCGCACCGCGGTCGCCGATGCGCTGGCGTCGAAGACGGCCAAGGCGTAG

Upstream 100 bases:

>100_bases
TATACGGTTCTTCAACCGGCGCGATCTCGCCGCCGGCAGTGCATGATGATTCCGGCCTCGTGGCTCGCCCGCGGGGCCCA
CGAGACGGAGACGAAACGAC

Downstream 100 bases:

>100_bases
CAGGCTTCCGCGCGCCGGCTCGAGCTGGCGCGCACCGTCCGGGCCCTCGGCGCGCCAGAGAACGCACGACGATTGTCGAT
GTCACGCCTCGCTGCCGGGA

Product: putative two-component response regulator

Products: NA

Alternate protein names: Stage 0 sporulation protein F [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSRVLIADDEDSMRTLVARAIAMDGHETVTAQDGAEALDILTRENGAFDLLLTDIQMPIMDGIALALSAARDFPDLTILL
MTGFAHQRERASNLNAIAHDVITKPFSVADIRTAVADALASKTAKA

Sequences:

>Translated_126_residues
MSRVLIADDEDSMRTLVARAIAMDGHETVTAQDGAEALDILTRENGAFDLLLTDIQMPIMDGIALALSAARDFPDLTILL
MTGFAHQRERASNLNAIAHDVITKPFSVADIRTAVADALASKTAKA
>Mature_125_residues
SRVLIADDEDSMRTLVARAIAMDGHETVTAQDGAEALDILTRENGAFDLLLTDIQMPIMDGIALALSAARDFPDLTILLM
TGFAHQRERASNLNAIAHDVITKPFSVADIRTAVADALASKTAKA

Specific function: Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=126, Percent_Identity=35.7142857142857, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=37.1681415929204, Blast_Score=62, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13479; Mature: 13347

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVLIADDEDSMRTLVARAIAMDGHETVTAQDGAEALDILTRENGAFDLLLTDIQMPIM
CCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCCCCEEEEEECHHHHHH
DGIALALSAARDFPDLTILLMTGFAHQRERASNLNAIAHDVITKPFSVADIRTAVADALA
HHHHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SKTAKA
HHCCCC
>Mature Secondary Structure 
SRVLIADDEDSMRTLVARAIAMDGHETVTAQDGAEALDILTRENGAFDLLLTDIQMPIM
CCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCCCCEEEEEECHHHHHH
DGIALALSAARDFPDLTILLMTGFAHQRERASNLNAIAHDVITKPFSVADIRTAVADALA
HHHHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SKTAKA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7514590 [H]