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The map label for this gene is greB [H]

Identifier: 146343253

GI number: 146343253

Start: 6725294

End: 6725818

Strand: Direct

Name: greB [H]

Synonym: BRADO6473

Alternate gene names: 146343253

Gene position: 6725294-6725818 (Clockwise)

Preceding gene: 146343252

Following gene: 146343254

Centisome position: 90.19

GC content: 70.29

Gene sequence:

>525_bases
GTGCGACGGCTGCAGGACCTGCGGCCGCTGGAGATCTGCCGCGTGAGCGTTGCATTCACCAAGGAAGAGAGCGCGGAGAC
CGCGGCGGAGACGATGCTGCCGGACCGTCCGGTCTCGCCGCATCCGAACCTCGTCACGGAGACCGGGCTGAAGGCGCTGG
AGGATCAGCTGCATCAGGCGCAGGCCGCCTATGAGGCGGCGCAGGCGATCGCGGACGTCAACGAGCGCCGCCGCGAGTCG
GCGCTGCCGCTGCGCGACGCGCGCTATTTCGCGGCCCGGCTGCGCACCGCCCAGCTGATGCCGCCCCCCTCCTCCACCGC
GGCGATCGCGTTCGGCAGCACGGTGACGTTCCGGCGCGGCGACGGCCGAACCCAGACCTATCGCATCGTTGGCGAGGACG
AGGCCGATCCCAAGCACGGAACGATCTCGCATGTCTCGCCGGTGGCGCGGCGGCTGATGGGCAAGACGGCCGGCGATGTC
GTCGAGGTGACCGGACAGGAGCTGGAGATCCTCCGGATTGACTGA

Upstream 100 bases:

>100_bases
CACGCCTCGCTGCCGGGATCCTGCGGATCCGCCCCGAGCGGATGCCGTGCCGGGCGGCCGACGGAACCCACTTGAAATCC
CGATCGGCGGCATGATCTCT

Downstream 100 bases:

>100_bases
CCCCTCTCAGGCGGGCGTGCTGAAGCTCGGGATCATCTCCGACACGCATGGACTGCTGCGGCCGGAGGCGGAGCGGTGTC
TTTCCGGCGTCGACCATATC

Product: transcription elongation factor regulatory protein

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MRRLQDLRPLEICRVSVAFTKEESAETAAETMLPDRPVSPHPNLVTETGLKALEDQLHQAQAAYEAAQAIADVNERRRES
ALPLRDARYFAARLRTAQLMPPPSSTAAIAFGSTVTFRRGDGRTQTYRIVGEDEADPKHGTISHVSPVARRLMGKTAGDV
VEVTGQELEILRID

Sequences:

>Translated_174_residues
MRRLQDLRPLEICRVSVAFTKEESAETAAETMLPDRPVSPHPNLVTETGLKALEDQLHQAQAAYEAAQAIADVNERRRES
ALPLRDARYFAARLRTAQLMPPPSSTAAIAFGSTVTFRRGDGRTQTYRIVGEDEADPKHGTISHVSPVARRLMGKTAGDV
VEVTGQELEILRID
>Mature_174_residues
MRRLQDLRPLEICRVSVAFTKEESAETAAETMLPDRPVSPHPNLVTETGLKALEDQLHQAQAAYEAAQAIADVNERRRES
ALPLRDARYFAARLRTAQLMPPPSSTAAIAFGSTVTFRRGDGRTQTYRIVGEDEADPKHGTISHVSPVARRLMGKTAGDV
VEVTGQELEILRID

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

None

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 19189; Mature: 19189

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLQDLRPLEICRVSVAFTKEESAETAAETMLPDRPVSPHPNLVTETGLKALEDQLHQA
CCCCHHCCCHHHHEEHHEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QAAYEAAQAIADVNERRRESALPLRDARYFAARLRTAQLMPPPSSTAAIAFGSTVTFRRG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECC
DGRTQTYRIVGEDEADPKHGTISHVSPVARRLMGKTAGDVVEVTGQELEILRID
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECC
>Mature Secondary Structure
MRRLQDLRPLEICRVSVAFTKEESAETAAETMLPDRPVSPHPNLVTETGLKALEDQLHQA
CCCCHHCCCHHHHEEHHEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QAAYEAAQAIADVNERRRESALPLRDARYFAARLRTAQLMPPPSSTAAIAFGSTVTFRRG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECC
DGRTQTYRIVGEDEADPKHGTISHVSPVARRLMGKTAGDVVEVTGQELEILRID
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]