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The map label for this gene is gspG [H]

Identifier: 146343148

GI number: 146343148

Start: 6598744

End: 6599220

Strand: Reverse

Name: gspG [H]

Synonym: BRADO6346

Alternate gene names: 146343148

Gene position: 6599220-6598744 (Counterclockwise)

Preceding gene: 146343149

Following gene: 146343147

Centisome position: 88.5

GC content: 64.78

Gene sequence:

>477_bases
ATGAGCTCGATGACGCAAACCACGCCGGCAAAGCATCCACGCCGGGAGAGCGAGCGCGGCTTCACGCTGGTCGAGATGCT
GGTGGTGATCACCATCATCGGCTTGATCATGGGGCTGATCGGCCCGCGCGTCCTCAATTATCTCAGCGAGTCCAAGACCA
AGGCCGCGCGGATCCAGTTGCAGAGCTTCTCCAGCGCGCTCGATCTGTTCTATCTCGACGTCGGCCGCTATCCCTCGACC
TCGGAGGGTCTCGCCGCGCTTGCGCAGCGTCCGGCCGGCTTGAACACGTGGAACGGGCCATATCTGAAGGGCGGAGCGGT
GCCGAAGGATCCGTGGAACGCGGCCTATGTGTATCGTGCACCGGGCGACCACGGCCCCTTTGACATCCTGTCCTACGGGG
CCGACGGCCAGGAAGGCGGCACCGGCACCGCGGCCGATATCGTTCTGGGAACACAGACGAGCGCGCGCGGTGAGTGA

Upstream 100 bases:

>100_bases
TGCTGATCAGCGTCGTCGTCGGCGGCCTGATCGTGTCGATCATGACGGCGCTGCTCTCGGTCACACAACTCGTCGGCTGA
CCTGGTCCAAGGAGAATGAG

Downstream 100 bases:

>100_bases
CGGGCGGCAGCGCGGCTTCACGCTTCTCGAGATGGTTTGCGTGCTTGCAATCATCGCGATGCTGGCGTCCGTCGCGCTGC
CCTATCTGCCACGGCAGACG

Product: general secretion pathway protein G

Products: NA

Alternate protein names: PilD-dependent protein pddA [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSSMTQTTPAKHPRRESERGFTLVEMLVVITIIGLIMGLIGPRVLNYLSESKTKAARIQLQSFSSALDLFYLDVGRYPST
SEGLAALAQRPAGLNTWNGPYLKGGAVPKDPWNAAYVYRAPGDHGPFDILSYGADGQEGGTGTAADIVLGTQTSARGE

Sequences:

>Translated_158_residues
MSSMTQTTPAKHPRRESERGFTLVEMLVVITIIGLIMGLIGPRVLNYLSESKTKAARIQLQSFSSALDLFYLDVGRYPST
SEGLAALAQRPAGLNTWNGPYLKGGAVPKDPWNAAYVYRAPGDHGPFDILSYGADGQEGGTGTAADIVLGTQTSARGE
>Mature_157_residues
SSMTQTTPAKHPRRESERGFTLVEMLVVITIIGLIMGLIGPRVLNYLSESKTKAARIQLQSFSSALDLFYLDVGRYPSTS
EGLAALAQRPAGLNTWNGPYLKGGAVPKDPWNAAYVYRAPGDHGPFDILSYGADGQEGGTGTAADIVLGTQTSARGE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2165

COG function: function code NU; Type II secretory pathway, pseudopilin PulG

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP G family [H]

Homologues:

Organism=Escherichia coli, GI1789725, Length=136, Percent_Identity=37.5, Blast_Score=77, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000983
- InterPro:   IPR012902
- InterPro:   IPR001120
- InterPro:   IPR013545
- InterPro:   IPR010054 [H]

Pfam domain/function: PF08334 GSPII_G; PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 16861; Mature: 16730

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00409 PROKAR_NTER_METHYL ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSMTQTTPAKHPRRESERGFTLVEMLVVITIIGLIMGLIGPRVLNYLSESKTKAARIQL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSFSSALDLFYLDVGRYPSTSEGLAALAQRPAGLNTWNGPYLKGGAVPKDPWNAAYVYRA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCEEEEEC
PGDHGPFDILSYGADGQEGGTGTAADIVLGTQTSARGE
CCCCCCHHHEECCCCCCCCCCCCHHEEEECCCCCCCCC
>Mature Secondary Structure 
SSMTQTTPAKHPRRESERGFTLVEMLVVITIIGLIMGLIGPRVLNYLSESKTKAARIQL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSFSSALDLFYLDVGRYPSTSEGLAALAQRPAGLNTWNGPYLKGGAVPKDPWNAAYVYRA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCEEEEEC
PGDHGPFDILSYGADGQEGGTGTAADIVLGTQTSARGE
CCCCCCHHHEECCCCCCCCCCCCHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1588814; 1309616; 10984043 [H]