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The map label for this gene is gspF [H]

Identifier: 146343149

GI number: 146343149

Start: 6599241

End: 6600449

Strand: Reverse

Name: gspF [H]

Synonym: BRADO6347

Alternate gene names: 146343149

Gene position: 6600449-6599241 (Counterclockwise)

Preceding gene: 146343150

Following gene: 146343148

Centisome position: 88.52

GC content: 67.82

Gene sequence:

>1209_bases
GTGCCGAGCTACCGCTATCGCGCTTTGACACAGTCCGGCGAGATCGTCGTCGGATCGCTCGATGCGCCGTCGCGCGGCGA
GGTCGAGCGCCGCATCTCCTATCTCCAGCTGCTTCCGATCGAGACGGTCGAAGAGAAGGCCAGGACCGCGTCATCAGCCG
GACTGTCGTTCGGCGGTCCGTCCGCAGTCGATGTGACCACCTTCACCCGCGATCTCGCATTGCTGCTCAAGGCCGGCGCG
CGGCTCGACGATGCCCTCGATCTGCTTTCGAACGATTCCGACATCGGCCGGCTCCGGCCGGTGGTCGCGCGGCTGCGGGC
GGCCATCATGAGCGGCGAGAGCTTTGCCGACGCCGCGGCCGCTCAGCCCTTGTTGTTCTCACCGATGTATGTCGCGCTGA
TGCGCGTGGGCGAGGCCTCGGGAACGCTCGATCAGGTGCTGACCGCACTTGCCGGCGAACGCGAGCGCTCCGAGGCGACG
CGCCGCAAGCTGACCGACGCGATGCAGTACCCCGCTTTCGTATTCCTGGCTGCGATCGGCGTGATGCTGTTCTTCCTCGT
GGCCGTGCTGCCGAATTTCTCCGCCGTGCTGCGCGATTTCGGCGGCCGCGCCGACACCGCGCTGGGCTTCTTCATGTCGC
TATCCGATATCCTGCGCGACAACGCCGCCGTGATCACGCTTGGTTGTGCGGCGCTGCTGGTGGCCGGCTGGTGGCTGCTG
CGCCAGGCCAACGTTCGCGCTGCTCTGACCAGTGGCCTTGCGGTGCTGCCCGGCATCGGCGGCATTCTCACCTTCTATCG
AACGAGCCTGTTCTGCCGCAATCTCGGTTTGCTGCTCGGCTGCGGAGTGACGCTGAGCGCGGCCTTGCGCATCCTGGTCG
ATGTGATGGCGGTGACCTCGTCGCGCGCGCCCTGGTCGGCCGCCGCCGATCGCGTGCGCCACGGCGGCAAGCTGTCCCAG
GCGCTGTCGGCCGACGACATGCTGCCGCCGATGGCGCTGCGCATGCTGCGGCTCGGCGAGGAGACCGGCCAGCTTCCGAC
GCTCTCGACCCGCATCGCCGAGTTCTACGAGGCCAAGCTGCAGCGCGCGCTCGACCGCGTCGTCGGCATCGTCGGCCCTG
CCGCCATCCTGCTGATCAGCGTCGTCGTCGGCGGCCTGATCGTGTCGATCATGACGGCGCTGCTCTCGGTCACACAACTC
GTCGGCTGA

Upstream 100 bases:

>100_bases
GCATGACGACGATGCTCGACGACGGGGTCGCCAAATGCCGCGCCGGCGTCACCTCGGCGTCCGAACTGTTCCGTGTCCTC
GTCGTGCGTTGAGGTGGATC

Downstream 100 bases:

>100_bases
CCTGGTCCAAGGAGAATGAGATGAGCTCGATGACGCAAACCACGCCGGCAAAGCATCCACGCCGGGAGAGCGAGCGCGGC
TTCACGCTGGTCGAGATGCT

Product: general secretion pathway protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MPSYRYRALTQSGEIVVGSLDAPSRGEVERRISYLQLLPIETVEEKARTASSAGLSFGGPSAVDVTTFTRDLALLLKAGA
RLDDALDLLSNDSDIGRLRPVVARLRAAIMSGESFADAAAAQPLLFSPMYVALMRVGEASGTLDQVLTALAGERERSEAT
RRKLTDAMQYPAFVFLAAIGVMLFFLVAVLPNFSAVLRDFGGRADTALGFFMSLSDILRDNAAVITLGCAALLVAGWWLL
RQANVRAALTSGLAVLPGIGGILTFYRTSLFCRNLGLLLGCGVTLSAALRILVDVMAVTSSRAPWSAAADRVRHGGKLSQ
ALSADDMLPPMALRMLRLGEETGQLPTLSTRIAEFYEAKLQRALDRVVGIVGPAAILLISVVVGGLIVSIMTALLSVTQL
VG

Sequences:

>Translated_402_residues
MPSYRYRALTQSGEIVVGSLDAPSRGEVERRISYLQLLPIETVEEKARTASSAGLSFGGPSAVDVTTFTRDLALLLKAGA
RLDDALDLLSNDSDIGRLRPVVARLRAAIMSGESFADAAAAQPLLFSPMYVALMRVGEASGTLDQVLTALAGERERSEAT
RRKLTDAMQYPAFVFLAAIGVMLFFLVAVLPNFSAVLRDFGGRADTALGFFMSLSDILRDNAAVITLGCAALLVAGWWLL
RQANVRAALTSGLAVLPGIGGILTFYRTSLFCRNLGLLLGCGVTLSAALRILVDVMAVTSSRAPWSAAADRVRHGGKLSQ
ALSADDMLPPMALRMLRLGEETGQLPTLSTRIAEFYEAKLQRALDRVVGIVGPAAILLISVVVGGLIVSIMTALLSVTQL
VG
>Mature_401_residues
PSYRYRALTQSGEIVVGSLDAPSRGEVERRISYLQLLPIETVEEKARTASSAGLSFGGPSAVDVTTFTRDLALLLKAGAR
LDDALDLLSNDSDIGRLRPVVARLRAAIMSGESFADAAAAQPLLFSPMYVALMRVGEASGTLDQVLTALAGERERSEATR
RKLTDAMQYPAFVFLAAIGVMLFFLVAVLPNFSAVLRDFGGRADTALGFFMSLSDILRDNAAVITLGCAALLVAGWWLLR
QANVRAALTSGLAVLPGIGGILTFYRTSLFCRNLGLLLGCGVTLSAALRILVDVMAVTSSRAPWSAAADRVRHGGKLSQA
LSADDMLPPMALRMLRLGEETGQLPTLSTRIAEFYEAKLQRALDRVVGIVGPAAILLISVVVGGLIVSIMTALLSVTQLV
G

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=403, Percent_Identity=29.2803970223325, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1786295, Length=362, Percent_Identity=24.8618784530387, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 42670; Mature: 42539

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSYRYRALTQSGEIVVGSLDAPSRGEVERRISYLQLLPIETVEEKARTASSAGLSFGGP
CCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
SAVDVTTFTRDLALLLKAGARLDDALDLLSNDSDIGRLRPVVARLRAAIMSGESFADAAA
CCEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AQPLLFSPMYVALMRVGEASGTLDQVLTALAGERERSEATRRKLTDAMQYPAFVFLAAIG
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLFFLVAVLPNFSAVLRDFGGRADTALGFFMSLSDILRDNAAVITLGCAALLVAGWWLL
HHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RQANVRAALTSGLAVLPGIGGILTFYRTSLFCRNLGLLLGCGVTLSAALRILVDVMAVTS
HHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
SRAPWSAAADRVRHGGKLSQALSADDMLPPMALRMLRLGEETGQLPTLSTRIAEFYEAKL
CCCCHHHHHHHHHCCCHHHHHHCHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QRALDRVVGIVGPAAILLISVVVGGLIVSIMTALLSVTQLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PSYRYRALTQSGEIVVGSLDAPSRGEVERRISYLQLLPIETVEEKARTASSAGLSFGGP
CCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
SAVDVTTFTRDLALLLKAGARLDDALDLLSNDSDIGRLRPVVARLRAAIMSGESFADAAA
CCEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AQPLLFSPMYVALMRVGEASGTLDQVLTALAGERERSEATRRKLTDAMQYPAFVFLAAIG
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLFFLVAVLPNFSAVLRDFGGRADTALGFFMSLSDILRDNAAVITLGCAALLVAGWWLL
HHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RQANVRAALTSGLAVLPGIGGILTFYRTSLFCRNLGLLLGCGVTLSAALRILVDVMAVTS
HHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
SRAPWSAAADRVRHGGKLSQALSADDMLPPMALRMLRLGEETGQLPTLSTRIAEFYEAKL
CCCCHHHHHHHHHCCCHHHHHHCHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QRALDRVVGIVGPAAILLISVVVGGLIVSIMTALLSVTQLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1944223; 12024217 [H]