Click here to switch to the map view.

The map label for this gene is rhmD [H]

Identifier: 146343127

GI number: 146343127

Start: 6577213

End: 6578319

Strand: Direct

Name: rhmD [H]

Synonym: BRADO6325

Alternate gene names: 146343127

Gene position: 6577213-6578319 (Clockwise)

Preceding gene: 146343126

Following gene: 146343128

Centisome position: 88.21

GC content: 68.83

Gene sequence:

>1107_bases
ATGCGCGCGACCGTTCCGATCGAGAGCGTCGCTGTCGGCGCCTTCGAGATTCCGACCGACCGGCCGGAGGCCGACGGAAC
GATCAGCTGGACGTCGACGACGCTGGTGCTGGTCGAAGTGTTCGCCCGCGGCAAGATCGGCATCGGCTACACCTATGCCC
ATGCCTGCATCGGCCGGCTGATCTCCGACACGCTCGCGCCGCTGGTCGAAGGCAGCGACGCGCTGGCGCCTGAACACACC
TGGGCCGTGATGCAGCGCGCGATCCGCAACCTCGGCCGGCAAGGTCTTGTGGCCACCGCGATCTCGGCTGTCGATACCGC
GCTCCACGATCTCAAGGCGCGGCTGCTGGATCTGCCGCTCTGCGCGCTGCTCGGCAGCTTTCGCCAGGAGATCCCGATCT
ACGGCAGCGGCGGCTTCACCAGCTATTCGGACGAAGAGCTGCGCCGCCAGCTGGCCGGATGGGTGTTCGAAGAGCGCTGC
GCCTGGGTCAAGATGAAGGTCGGCTCCGAGCCCGCGCGCGATCCGCATCGAGTCGAGGTGGCGCGGCTGGCGATCGGCGA
GCGCGCCGCGCTGTTCGTCGATGCCAACGGCGCCTATTCGCCCAAGCAGGCGTTGGGACTCGCGCAGCCGTTAGCGGATC
TCGGCGTGAGCTGGCTCGAGGAGCCGGTGTCGTCGGATGATCTGCCTGGACTGCGCCTGGTGCGCGAGCGCGCTCCCGCG
GGCATGGACGTGGCCGCCGGCGAATACAGCTACACGACGGACCAGGTGCGGATGATGCTGCAGGCGGGCGCGGTCGATGT
CCAGCAGGCCGACATCACCCGCTGCGGCGGCGTCACCGGCTTCCGGCAGATCGCCGCGCTGTGCGATGCCTTCCACACCG
ATCTCTCCGGCCATTGCGCGCCGTCCTTGCATCTGCACGCCGCCTGCGCCGCGCCGCGGCTGCGCCACCTCGAATGGTTT
CACGACCATGTCCGCATCGAGCACATGCTGTTCGACGGCGCGCCGCAGCCGAAGGACGGCAGGATCCGTCCCGACCTCAG
CCGTCCCGGCCATGGCCTGACATTCAAGCACGCCGATGCGGCGCGCTACGCCGTGAGTGCGACATGA

Upstream 100 bases:

>100_bases
AGAACTTCATCAGCATGATTCCGTCGGAGCCGGAGCTCGGCAGCGTGCTGAAGAACAGCGCCAAGGAGCTCGTCACCAGC
ATCCTGCCGGGGAAAGACTG

Downstream 100 bases:

>100_bases
GCAACGACACCAATTGGCTGGTGGGAGCCGGCCTCGCCATCGCCGCGACGGCGATGCTGCAACCGCGGCGTCGGACCACA
CTCGCACGGCCGACAGCGAG

Product: putative mandelate racemase/muconate lactonizing family protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MRATVPIESVAVGAFEIPTDRPEADGTISWTSTTLVLVEVFARGKIGIGYTYAHACIGRLISDTLAPLVEGSDALAPEHT
WAVMQRAIRNLGRQGLVATAISAVDTALHDLKARLLDLPLCALLGSFRQEIPIYGSGGFTSYSDEELRRQLAGWVFEERC
AWVKMKVGSEPARDPHRVEVARLAIGERAALFVDANGAYSPKQALGLAQPLADLGVSWLEEPVSSDDLPGLRLVRERAPA
GMDVAAGEYSYTTDQVRMMLQAGAVDVQQADITRCGGVTGFRQIAALCDAFHTDLSGHCAPSLHLHAACAAPRLRHLEWF
HDHVRIEHMLFDGAPQPKDGRIRPDLSRPGHGLTFKHADAARYAVSAT

Sequences:

>Translated_368_residues
MRATVPIESVAVGAFEIPTDRPEADGTISWTSTTLVLVEVFARGKIGIGYTYAHACIGRLISDTLAPLVEGSDALAPEHT
WAVMQRAIRNLGRQGLVATAISAVDTALHDLKARLLDLPLCALLGSFRQEIPIYGSGGFTSYSDEELRRQLAGWVFEERC
AWVKMKVGSEPARDPHRVEVARLAIGERAALFVDANGAYSPKQALGLAQPLADLGVSWLEEPVSSDDLPGLRLVRERAPA
GMDVAAGEYSYTTDQVRMMLQAGAVDVQQADITRCGGVTGFRQIAALCDAFHTDLSGHCAPSLHLHAACAAPRLRHLEWF
HDHVRIEHMLFDGAPQPKDGRIRPDLSRPGHGLTFKHADAARYAVSAT
>Mature_368_residues
MRATVPIESVAVGAFEIPTDRPEADGTISWTSTTLVLVEVFARGKIGIGYTYAHACIGRLISDTLAPLVEGSDALAPEHT
WAVMQRAIRNLGRQGLVATAISAVDTALHDLKARLLDLPLCALLGSFRQEIPIYGSGGFTSYSDEELRRQLAGWVFEERC
AWVKMKVGSEPARDPHRVEVARLAIGERAALFVDANGAYSPKQALGLAQPLADLGVSWLEEPVSSDDLPGLRLVRERAPA
GMDVAAGEYSYTTDQVRMMLQAGAVDVQQADITRCGGVTGFRQIAALCDAFHTDLSGHCAPSLHLHAACAAPRLRHLEWF
HDHVRIEHMLFDGAPQPKDGRIRPDLSRPGHGLTFKHADAARYAVSAT

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI186972148, Length=172, Percent_Identity=29.0697674418605, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI42544119, Length=172, Percent_Identity=29.0697674418605, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI226510960, Length=273, Percent_Identity=27.4725274725275, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994953, Length=320, Percent_Identity=25, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 39844; Mature: 39844

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRATVPIESVAVGAFEIPTDRPEADGTISWTSTTLVLVEVFARGKIGIGYTYAHACIGRL
CCCCCCHHHHEECEEECCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ISDTLAPLVEGSDALAPEHTWAVMQRAIRNLGRQGLVATAISAVDTALHDLKARLLDLPL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CALLGSFRQEIPIYGSGGFTSYSDEELRRQLAGWVFEERCAWVKMKVGSEPARDPHRVEV
HHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHH
ARLAIGERAALFVDANGAYSPKQALGLAQPLADLGVSWLEEPVSSDDLPGLRLVRERAPA
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHCCC
GMDVAAGEYSYTTDQVRMMLQAGAVDVQQADITRCGGVTGFRQIAALCDAFHTDLSGHCA
CCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
PSLHLHAACAAPRLRHLEWFHDHVRIEHMLFDGAPQPKDGRIRPDLSRPGHGLTFKHADA
CCCEEHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEECCCH
ARYAVSAT
HCEEEECC
>Mature Secondary Structure
MRATVPIESVAVGAFEIPTDRPEADGTISWTSTTLVLVEVFARGKIGIGYTYAHACIGRL
CCCCCCHHHHEECEEECCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ISDTLAPLVEGSDALAPEHTWAVMQRAIRNLGRQGLVATAISAVDTALHDLKARLLDLPL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CALLGSFRQEIPIYGSGGFTSYSDEELRRQLAGWVFEERCAWVKMKVGSEPARDPHRVEV
HHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHH
ARLAIGERAALFVDANGAYSPKQALGLAQPLADLGVSWLEEPVSSDDLPGLRLVRERAPA
HHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHCCC
GMDVAAGEYSYTTDQVRMMLQAGAVDVQQADITRCGGVTGFRQIAALCDAFHTDLSGHCA
CCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
PSLHLHAACAAPRLRHLEWFHDHVRIEHMLFDGAPQPKDGRIRPDLSRPGHGLTFKHADA
CCCEEHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEECCCH
ARYAVSAT
HCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA