Click here to switch to the map view.

The map label for this gene is ydaP [H]

Identifier: 146343126

GI number: 146343126

Start: 6575438

End: 6577213

Strand: Direct

Name: ydaP [H]

Synonym: BRADO6324

Alternate gene names: 146343126

Gene position: 6575438-6577213 (Clockwise)

Preceding gene: 146343125

Following gene: 146343127

Centisome position: 88.18

GC content: 66.33

Gene sequence:

>1776_bases
ATGAACGTCAGCGAATTCGTTTGGCATCGCCTGTCCGAATGGGGTCTCCACCGTGTCTATGGATATCCCGGCGACGGGGT
CGGCGGGCTGGACGTTGCGCTCGACAAGGCAAAGGACGTGATGAGCTACGTCCAGGTGCGGCACGAGGAGATGGCCGCGT
TCATGGCTTCCGCACATGCCAAGTTCACCGGCCAGGTCGGGCTCTGCTACGCCACCTCCGGGCCCGGCGCGATCCATCTC
CTGAACGGGCTGTACGATGCGAAGATGGATCACGTCCCCGTGGTCGCTCTGGTCGGCCAGGCGGCGCGGGCCTCGATCGG
CGCGAACTATCAGCAGGAGGTCGACCTCAAGACGCTGTTCAAGGATGTCGCGGAATATGTGGAACAGGCAAGCGTGCCGG
AGCAGGTCCGCCATCTCGTCGACCGCGCGGTCCGCATCGCTCATACGCGACGAACGGTGACCTGCATCATCCTGCCGAAG
GATCTGCAGGAGATGGACTATGAGGATCCGCCGCTCACCCACGGCACGACGCACACCGGCCTCGGCTATGCCGGGCCGGC
GCAGGTTCCGGAGGATGCGCAGCTGCGCGCGGCCGCGGAGGTGCTGAACAGCGGCCGCAAGGTCGCGATGCTCATTGGCG
CCGGCGCGCTCGACGCGACGGACGAGGTGATCGCCGTGGCCGAGCGGCTGCAGGCCGGTGTCGCGAAAGCGCTGCTCGGC
AAGGCCGCGGTGCCCGACGATCTGCCGTTCGTCACCGGCTCGATCGGCCTGCTCGGCTCCAAGCCGAGCTGGGACATGAT
GAAGTCCTGCGACACCTTCCTGATGGTCGGCTCGGCGTTCCCCTATAGCGAGTTCCTGCCGAAGCCGGGCGATGCGCGCG
GCGTGCAGATCGACATCGACGGCACGCGGCTCAGCCTGCGCTATCCGATGGAGGTCAATCTCGTCGGCGACAGCGCGGCG
ACGTTGCGCGCGCTGCTGCCGCTCTTGGAGCAGAAGCCGAAGGACGGCTGGCGCAGCGGCATCGAGGACGGCGTTCGCGA
CTGGTGGTCGACGCTGGAGCGCCGCGCGATGGATTCGGCCGATCCACTCAATCCGCAGCGCGTGTTCTGGGAGCTGTCGC
CTCGCCTGCCCGATAATGCGATCATCACCGCCGATTCCGGCTCGGCCGCCAACTGGTACGCGCGCGACCTGAAGATCCGG
CGCGGCATGAAGGCGTCGCTGTCCGGCGGCCTCGCCTCGCTCGGCGCCGGCACGCCCTACGCGCTCGCCGCCAAGATGGC
CTATCCCGAGCGCACCGTGATCGCCTGCATGGGTGACGGCGCGATGCAGATGAACGGGCTGAACGTGATGATCACGATCG
GCAAATATTGGCGCGAGTGGAGCAACCCGCGCCTCATCGTGCTCGTGCTCAACAACCGCGATCTCAACCAGGTCACCTGG
GAGGAGCGCATCCAGCTCGGCACCGGCAAGACGCTCTCGACGCAGAGCATCCCGGACTTCTCCTACCATCGCTATGCCGA
GCTGATCGGCCTCAAGGGCATCTTCGTCGACGATCCCGAGCACGTCGGCGCCGCCTGGGACGAAGCGCTCGCGGCCGACC
GGCCCGTCATCCTCGAAGCCTACACCGATCCGAACGTGCCGCCGCTGCCGCCACATATCACGCTGAAGGATGCCAAGAAC
TTCATCAGCATGATTCCGTCGGAGCCGGAGCTCGGCAGCGTGCTGAAGAACAGCGCCAAGGAGCTCGTCACCAGCATCCT
GCCGGGGAAAGACTGA

Upstream 100 bases:

>100_bases
ACATGAATTCTCGTTAAAGCGGCAACGTTTGCCGCGACGGCACGTTTGATGGACACCAGCAGCGCCGCTCTCCGCAAACT
TCCGATCGAGGATCACGTCC

Downstream 100 bases:

>100_bases
TGCGCGCGACCGTTCCGATCGAGAGCGTCGCTGTCGGCGCCTTCGAGATTCCGACCGACCGGCCGGAGGCCGACGGAACG
ATCAGCTGGACGTCGACGAC

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MNVSEFVWHRLSEWGLHRVYGYPGDGVGGLDVALDKAKDVMSYVQVRHEEMAAFMASAHAKFTGQVGLCYATSGPGAIHL
LNGLYDAKMDHVPVVALVGQAARASIGANYQQEVDLKTLFKDVAEYVEQASVPEQVRHLVDRAVRIAHTRRTVTCIILPK
DLQEMDYEDPPLTHGTTHTGLGYAGPAQVPEDAQLRAAAEVLNSGRKVAMLIGAGALDATDEVIAVAERLQAGVAKALLG
KAAVPDDLPFVTGSIGLLGSKPSWDMMKSCDTFLMVGSAFPYSEFLPKPGDARGVQIDIDGTRLSLRYPMEVNLVGDSAA
TLRALLPLLEQKPKDGWRSGIEDGVRDWWSTLERRAMDSADPLNPQRVFWELSPRLPDNAIITADSGSAANWYARDLKIR
RGMKASLSGGLASLGAGTPYALAAKMAYPERTVIACMGDGAMQMNGLNVMITIGKYWREWSNPRLIVLVLNNRDLNQVTW
EERIQLGTGKTLSTQSIPDFSYHRYAELIGLKGIFVDDPEHVGAAWDEALAADRPVILEAYTDPNVPPLPPHITLKDAKN
FISMIPSEPELGSVLKNSAKELVTSILPGKD

Sequences:

>Translated_591_residues
MNVSEFVWHRLSEWGLHRVYGYPGDGVGGLDVALDKAKDVMSYVQVRHEEMAAFMASAHAKFTGQVGLCYATSGPGAIHL
LNGLYDAKMDHVPVVALVGQAARASIGANYQQEVDLKTLFKDVAEYVEQASVPEQVRHLVDRAVRIAHTRRTVTCIILPK
DLQEMDYEDPPLTHGTTHTGLGYAGPAQVPEDAQLRAAAEVLNSGRKVAMLIGAGALDATDEVIAVAERLQAGVAKALLG
KAAVPDDLPFVTGSIGLLGSKPSWDMMKSCDTFLMVGSAFPYSEFLPKPGDARGVQIDIDGTRLSLRYPMEVNLVGDSAA
TLRALLPLLEQKPKDGWRSGIEDGVRDWWSTLERRAMDSADPLNPQRVFWELSPRLPDNAIITADSGSAANWYARDLKIR
RGMKASLSGGLASLGAGTPYALAAKMAYPERTVIACMGDGAMQMNGLNVMITIGKYWREWSNPRLIVLVLNNRDLNQVTW
EERIQLGTGKTLSTQSIPDFSYHRYAELIGLKGIFVDDPEHVGAAWDEALAADRPVILEAYTDPNVPPLPPHITLKDAKN
FISMIPSEPELGSVLKNSAKELVTSILPGKD
>Mature_591_residues
MNVSEFVWHRLSEWGLHRVYGYPGDGVGGLDVALDKAKDVMSYVQVRHEEMAAFMASAHAKFTGQVGLCYATSGPGAIHL
LNGLYDAKMDHVPVVALVGQAARASIGANYQQEVDLKTLFKDVAEYVEQASVPEQVRHLVDRAVRIAHTRRTVTCIILPK
DLQEMDYEDPPLTHGTTHTGLGYAGPAQVPEDAQLRAAAEVLNSGRKVAMLIGAGALDATDEVIAVAERLQAGVAKALLG
KAAVPDDLPFVTGSIGLLGSKPSWDMMKSCDTFLMVGSAFPYSEFLPKPGDARGVQIDIDGTRLSLRYPMEVNLVGDSAA
TLRALLPLLEQKPKDGWRSGIEDGVRDWWSTLERRAMDSADPLNPQRVFWELSPRLPDNAIITADSGSAANWYARDLKIR
RGMKASLSGGLASLGAGTPYALAAKMAYPERTVIACMGDGAMQMNGLNVMITIGKYWREWSNPRLIVLVLNNRDLNQVTW
EERIQLGTGKTLSTQSIPDFSYHRYAELIGLKGIFVDDPEHVGAAWDEALAADRPVILEAYTDPNVPPLPPHITLKDAKN
FISMIPSEPELGSVLKNSAKELVTSILPGKD

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=557, Percent_Identity=23.5188509874327, Blast_Score=138, Evalue=2e-32,
Organism=Escherichia coli, GI1787096, Length=562, Percent_Identity=32.2064056939502, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI87081685, Length=556, Percent_Identity=30.5755395683453, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=25.4480286738351, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=25, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1788716, Length=581, Percent_Identity=22.5473321858864, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=23.5500878734622, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=573, Percent_Identity=23.5602094240838, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=25.1760563380282, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320816, Length=541, Percent_Identity=20.3327171903882, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922626, Length=578, Percent_Identity=23.1833910034602, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 64304; Mature: 64304

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSEFVWHRLSEWGLHRVYGYPGDGVGGLDVALDKAKDVMSYVQVRHEEMAAFMASAHA
CCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFTGQVGLCYATSGPGAIHLLNGLYDAKMDHVPVVALVGQAARASIGANYQQEVDLKTLF
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHCCCCHHHCCHHHHH
KDVAEYVEQASVPEQVRHLVDRAVRIAHTRRTVTCIILPKDLQEMDYEDPPLTHGTTHTG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCCC
LGYAGPAQVPEDAQLRAAAEVLNSGRKVAMLIGAGALDATDEVIAVAERLQAGVAKALLG
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
KAAVPDDLPFVTGSIGLLGSKPSWDMMKSCDTFLMVGSAFPYSEFLPKPGDARGVQIDID
CCCCCCCCCEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCEEEEEC
GTRLSLRYPMEVNLVGDSAATLRALLPLLEQKPKDGWRSGIEDGVRDWWSTLERRAMDSA
CCEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DPLNPQRVFWELSPRLPDNAIITADSGSAANWYARDLKIRRGMKASLSGGLASLGAGTPY
CCCCHHEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHCCCHHHHCCCCCH
ALAAKMAYPERTVIACMGDGAMQMNGLNVMITIGKYWREWSNPRLIVLVLNNRDLNQVTW
HHHHHHCCCCCEEEEEECCCCEEECCCEEEEEECHHHHHCCCCEEEEEEECCCCCCHHHH
EERIQLGTGKTLSTQSIPDFSYHRYAELIGLKGIFVDDPEHVGAAWDEALAADRPVILEA
HHHHCCCCCCEECCCCCCCCHHHHHHHHHCCCCEEECCHHHHCHHHHHHHHCCCCEEEEE
YTDPNVPPLPPHITLKDAKNFISMIPSEPELGSVLKNSAKELVTSILPGKD
CCCCCCCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVSEFVWHRLSEWGLHRVYGYPGDGVGGLDVALDKAKDVMSYVQVRHEEMAAFMASAHA
CCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFTGQVGLCYATSGPGAIHLLNGLYDAKMDHVPVVALVGQAARASIGANYQQEVDLKTLF
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHCCCCHHHCCHHHHH
KDVAEYVEQASVPEQVRHLVDRAVRIAHTRRTVTCIILPKDLQEMDYEDPPLTHGTTHTG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCCC
LGYAGPAQVPEDAQLRAAAEVLNSGRKVAMLIGAGALDATDEVIAVAERLQAGVAKALLG
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
KAAVPDDLPFVTGSIGLLGSKPSWDMMKSCDTFLMVGSAFPYSEFLPKPGDARGVQIDID
CCCCCCCCCEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCEEEEEC
GTRLSLRYPMEVNLVGDSAATLRALLPLLEQKPKDGWRSGIEDGVRDWWSTLERRAMDSA
CCEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DPLNPQRVFWELSPRLPDNAIITADSGSAANWYARDLKIRRGMKASLSGGLASLGAGTPY
CCCCHHEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHCCCHHHHCCCCCH
ALAAKMAYPERTVIACMGDGAMQMNGLNVMITIGKYWREWSNPRLIVLVLNNRDLNQVTW
HHHHHHCCCCCEEEEEECCCCEEECCCEEEEEECHHHHHCCCCEEEEEEECCCCCCHHHH
EERIQLGTGKTLSTQSIPDFSYHRYAELIGLKGIFVDDPEHVGAAWDEALAADRPVILEA
HHHHCCCCCCEECCCCCCCCHHHHHHHHHCCCCEEECCHHHHCHHHHHHHHCCCCEEEEE
YTDPNVPPLPPHITLKDAKNFISMIPSEPELGSVLKNSAKELVTSILPGKD
CCCCCCCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0017 {lecithin}} 0.03 {sodium} 11 {pyruvate}} 10 {pyruvate}} 0.0022 {lysolecithin}} 2 {ferricyanide}} 0.0012 {thiamine} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]