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The map label for this gene is ywbI [H]

Identifier: 146343029

GI number: 146343029

Start: 6453895

End: 6454785

Strand: Reverse

Name: ywbI [H]

Synonym: BRADO6217

Alternate gene names: 146343029

Gene position: 6454785-6453895 (Counterclockwise)

Preceding gene: 146343031

Following gene: 146343025

Centisome position: 86.56

GC content: 69.14

Gene sequence:

>891_bases
ATGCGGTTCGATCTCGTCGATCTCAGGCTGTTCATCGCCGTCGCCGAAGCACGCAGCATCACCGGCGGCGCCGATCGGGC
CCATCTGGCGCTGGCGTCGGCCAGCGCGCGCATCAAGGGCCTCGAGGAAACGTTCGGCGTGGCGCTGTTCAAGCGCGGTC
GTCGCGGCGTCGACCTGACCGCAGCGGGCGAAAGCCTGCTCGAGCACGCCCGCATCGTCATGGCGCATGTCGAGACCATG
CAGGGCGACTTGTCCGGTTTCGCATCCGGCCAGCGGGCCAATGTGCAGTTGCTCGCCAACACCGTCGGCCTTGCCGAGCA
TCTGCCGAAGGCGCTCGCGGCGTTTTTGCGTGCTCATCCCGAGATCAATGTCGACGTGGAGGAGCGCGAGAGCACCGAGA
TCGCGGAGGCGGTTGCGTCCGGTCGCGCCGATCTCGGCTTTGCCGCCGAGCATGCGCTGCCCGAGACGATCGAGCGCTTC
GCGTTCGGCGAGGACCGGCTGGTGCTGGTGGCTGGAAAACGCAGCGGCTTCGCAGGGCGCAGGCAGATCGATTTCAGCGA
AACCGCGGGGCAGGACTTCATCGGTCTCACCCAGGGGACCGCGCTCAACGTCCATATCGGTCGGCATGCGGCGCGGCTCG
GCATCCGCCAGCATGTCCGGGCCCGGCTGCGCGACTTCGACGCGATCTGCCAGATGGTGGCGGCCGGCATCGGCATCGCC
GTCGTGCCGGAAGCTGCGGCCCGGCGCTGCGCACGGACGATGCCGATCAGCCTCATTCCGTTGCGCGACGCCTGGGCGGA
CCGCAGGCTCGTGATGTGCGCCCGCAGCTTCAAGGCGCTGCCGCGCCCGGCCAAGCTGCTGGTCGAGCATCTGCGCGGCG
CGGCCGCGTGA

Upstream 100 bases:

>100_bases
TGCGGTATCGATCATGGCAATGACATTGACCCGAAGTTCGACCTCGTCACAATCTGAAGGTTACGGAGCGAGCCTTCGTC
CAGGACAGAGGCAAGAGACC

Downstream 100 bases:

>100_bases
GCCGTCAGGCTGCGCGGACGTTCTCGAGGAAATGGCCGACCTCGAGCTTCAGGCGGCTACTCTCCTCCGCCAGCGACTGC
GCCGCCGACAGCACCTGGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRFDLVDLRLFIAVAEARSITGGADRAHLALASASARIKGLEETFGVALFKRGRRGVDLTAAGESLLEHARIVMAHVETM
QGDLSGFASGQRANVQLLANTVGLAEHLPKALAAFLRAHPEINVDVEERESTEIAEAVASGRADLGFAAEHALPETIERF
AFGEDRLVLVAGKRSGFAGRRQIDFSETAGQDFIGLTQGTALNVHIGRHAARLGIRQHVRARLRDFDAICQMVAAGIGIA
VVPEAAARRCARTMPISLIPLRDAWADRRLVMCARSFKALPRPAKLLVEHLRGAAA

Sequences:

>Translated_296_residues
MRFDLVDLRLFIAVAEARSITGGADRAHLALASASARIKGLEETFGVALFKRGRRGVDLTAAGESLLEHARIVMAHVETM
QGDLSGFASGQRANVQLLANTVGLAEHLPKALAAFLRAHPEINVDVEERESTEIAEAVASGRADLGFAAEHALPETIERF
AFGEDRLVLVAGKRSGFAGRRQIDFSETAGQDFIGLTQGTALNVHIGRHAARLGIRQHVRARLRDFDAICQMVAAGIGIA
VVPEAAARRCARTMPISLIPLRDAWADRRLVMCARSFKALPRPAKLLVEHLRGAAA
>Mature_296_residues
MRFDLVDLRLFIAVAEARSITGGADRAHLALASASARIKGLEETFGVALFKRGRRGVDLTAAGESLLEHARIVMAHVETM
QGDLSGFASGQRANVQLLANTVGLAEHLPKALAAFLRAHPEINVDVEERESTEIAEAVASGRADLGFAAEHALPETIERF
AFGEDRLVLVAGKRSGFAGRRQIDFSETAGQDFIGLTQGTALNVHIGRHAARLGIRQHVRARLRDFDAICQMVAAGIGIA
VVPEAAARRCARTMPISLIPLRDAWADRRLVMCARSFKALPRPAKLLVEHLRGAAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=23.8683127572016, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787879, Length=289, Percent_Identity=23.8754325259516, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786713, Length=114, Percent_Identity=42.9824561403509, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=25.3061224489796, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788748, Length=299, Percent_Identity=22.4080267558528, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=255, Percent_Identity=23.921568627451, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787530, Length=252, Percent_Identity=23.015873015873, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787806, Length=267, Percent_Identity=24.3445692883895, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=239, Percent_Identity=21.7573221757322, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787128, Length=131, Percent_Identity=29.7709923664122, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=23.8866396761134, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787949, Length=243, Percent_Identity=23.0452674897119, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787589, Length=257, Percent_Identity=25.6809338521401, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31873; Mature: 31873

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDLVDLRLFIAVAEARSITGGADRAHLALASASARIKGLEETFGVALFKRGRRGVDLT
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
AAGESLLEHARIVMAHVETMQGDLSGFASGQRANVQLLANTVGLAEHLPKALAAFLRAHP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EINVDVEERESTEIAEAVASGRADLGFAAEHALPETIERFAFGEDRLVLVAGKRSGFAGR
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCCCC
RQIDFSETAGQDFIGLTQGTALNVHIGRHAARLGIRQHVRARLRDFDAICQMVAAGIGIA
CCCCCHHHCCCCCEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VVPEAAARRCARTMPISLIPLRDAWADRRLVMCARSFKALPRPAKLLVEHLRGAAA
ECCHHHHHHHHHCCCEEEEECHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MRFDLVDLRLFIAVAEARSITGGADRAHLALASASARIKGLEETFGVALFKRGRRGVDLT
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
AAGESLLEHARIVMAHVETMQGDLSGFASGQRANVQLLANTVGLAEHLPKALAAFLRAHP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EINVDVEERESTEIAEAVASGRADLGFAAEHALPETIERFAFGEDRLVLVAGKRSGFAGR
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCCCC
RQIDFSETAGQDFIGLTQGTALNVHIGRHAARLGIRQHVRARLRDFDAICQMVAAGIGIA
CCCCCHHHCCCCCEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VVPEAAARRCARTMPISLIPLRDAWADRRLVMCARSFKALPRPAKLLVEHLRGAAA
ECCHHHHHHHHHCCCEEEEECHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]