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The map label for this gene is rhtB [C]

Identifier: 146343031

GI number: 146343031

Start: 6455630

End: 6456265

Strand: Reverse

Name: rhtB [C]

Synonym: BRADO6219

Alternate gene names: 146343031

Gene position: 6456265-6455630 (Counterclockwise)

Preceding gene: 146343035

Following gene: 146343029

Centisome position: 86.58

GC content: 65.09

Gene sequence:

>636_bases
ATGCTCGGAATTCACGATCTGCCGCTTTTCCTGGTTTCCGGACTGCTGCTCAATATCATGCCCGGTCCAGATACGGCCTA
CATCGTCGGCCGCAGTGCGCAGATCGGCTGGCGGGGCGGCGCGGCCGCCGCCTTCGGGATCAGCGCGGGCTGCCTGGTGC
ATGTGCTCGGAAGCGCGATCGGGCTGTCGGCGTTGCTTACCGCATCGGCGACGGCCTTCGCCGCCGTGAAATGGGCAGGG
GCCGCCTATCTGGTGTATCTCGGCTTGAAGCAGATCCTGGCTCGCAGCCAAGACACGAACGCGACCGCACCCGCTGCGCT
CGCCATTGGGTTGCGCCAGGTGTTCTGGCAAGGCGCGCTCACGAATGTTCTCAATCCCAAGGTCGCGATGTTCTTTCTCG
CCTTCCTGCCGCAATTCGTCGCAGCTGACGCGCCGAATCAGGCATTGGCCTTTCTGCTGCTCGGGACGATCTTCGTGTTC
AACAGCACACTCTGGTGCCTGGGCGTCGCCATTGCGGCGGCCCGCGCCGCCCGCGGCGTGCGGAGTTCGCGCGTGGCGAT
GCTCTGGCTGAATCGCGCGATCGGTGGTCTGCTGATCTGTCTCGGCGTCCGCATCGCGCTTTCACACGCGCGCTGA

Upstream 100 bases:

>100_bases
CCGCACACGTGACCTTCGACGAGGCATTCTGGATACGTCGACCCGCACCAGCTCACGGTCGCTAAAAGTTTAGATCGATA
TCGTTAGGAAACGTTCAATC

Downstream 100 bases:

>100_bases
TCCCCAGGACAGTTCGATCGCGGCGCTGCCCGCGAGATAGACCCCGAGCAGGATCATCCCGATCAAGAACACGCGGCGGA
AGGCTTCGGCAGGCAGTCTC

Product: LysE family amino acid efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MLGIHDLPLFLVSGLLLNIMPGPDTAYIVGRSAQIGWRGGAAAAFGISAGCLVHVLGSAIGLSALLTASATAFAAVKWAG
AAYLVYLGLKQILARSQDTNATAPAALAIGLRQVFWQGALTNVLNPKVAMFFLAFLPQFVAADAPNQALAFLLLGTIFVF
NSTLWCLGVAIAAARAARGVRSSRVAMLWLNRAIGGLLICLGVRIALSHAR

Sequences:

>Translated_211_residues
MLGIHDLPLFLVSGLLLNIMPGPDTAYIVGRSAQIGWRGGAAAAFGISAGCLVHVLGSAIGLSALLTASATAFAAVKWAG
AAYLVYLGLKQILARSQDTNATAPAALAIGLRQVFWQGALTNVLNPKVAMFFLAFLPQFVAADAPNQALAFLLLGTIFVF
NSTLWCLGVAIAAARAARGVRSSRVAMLWLNRAIGGLLICLGVRIALSHAR
>Mature_211_residues
MLGIHDLPLFLVSGLLLNIMPGPDTAYIVGRSAQIGWRGGAAAAFGISAGCLVHVLGSAIGLSALLTASATAFAAVKWAG
AAYLVYLGLKQILARSQDTNATAPAALAIGLRQVFWQGALTNVLNPKVAMFFLAFLPQFVAADAPNQALAFLLLGTIFVF
NSTLWCLGVAIAAARAARGVRSSRVAMLWLNRAIGGLLICLGVRIALSHAR

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=198, Percent_Identity=29.7979797979798, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788099, Length=204, Percent_Identity=25.4901960784314, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21926; Mature: 21926

Theoretical pI: Translated: 11.38; Mature: 11.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGIHDLPLFLVSGLLLNIMPGPDTAYIVGRSAQIGWRGGAAAAFGISAGCLVHVLGSAI
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GLSALLTASATAFAAVKWAGAAYLVYLGLKQILARSQDTNATAPAALAIGLRQVFWQGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
TNVLNPKVAMFFLAFLPQFVAADAPNQALAFLLLGTIFVFNSTLWCLGVAIAAARAARGV
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
RSSRVAMLWLNRAIGGLLICLGVRIALSHAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLGIHDLPLFLVSGLLLNIMPGPDTAYIVGRSAQIGWRGGAAAAFGISAGCLVHVLGSAI
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GLSALLTASATAFAAVKWAGAAYLVYLGLKQILARSQDTNATAPAALAIGLRQVFWQGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
TNVLNPKVAMFFLAFLPQFVAADAPNQALAFLLLGTIFVFNSTLWCLGVAIAAARAARGV
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
RSSRVAMLWLNRAIGGLLICLGVRIALSHAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]